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Proteome Profiler Human Angiogenesis Array Kit

Contains 4 membranes - each spotted in duplicate with 55 different angiogenesis antibodies
Profiling Angiogenesis-Related Proteins in Human Cancer Tissues.
(3)
The Human Angiogenesis Array detects multiple analytes in cell culture supernates.
The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma.
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ARY007

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Bio-Techne Corporation
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Citations (115)

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Customer Reviews (8)

4.9 out of 5
8 customer ratings
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  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 02/07/2019
    Proteome Profiler Human Angiogenesis Array Kit Serum ARY007
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 02/06/2019
    Worked well to look at THP-1 angiogenesis factors.
    Proteome Profiler Human Angiogenesis Array Kit Serum ARY007
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 10/22/2018
    It worked well in cell culture supernatants using 125 ug of total protein per membrane.
    Proteome Profiler Human Angiogenesis Array Kit Serum ARY007
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 08/08/2018
    Proteome Profiler Human Angiogenesis Array Kit Serum ARY007
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 05/17/2018
    Used it on Conditioned medium collected from Breast Cancer Cells treated with Angiotensin II to look into its effect on Angiogenesis. Angiogenesis array worked really well and very easy to use. It provided some new promising gene targets which we did not know before.
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 04/01/2018
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 10/30/2017
    Proteome Profiler Human Angiogenesis Array Kit Serum ARY007
  • Proteome Profiler Human Angiogenesis Array Kit
    Name: Anonymous
    Verified Customer | Posted 12/06/2016
    Human Angiogenesis Antibody Array was used to evaluate mesenchymal stem cells secretion profiles when seeded in various extracellular matrices. Great tool that ganarates lots of data!

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FAQs

  • Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  • What are the associated pathways each factor is involved in?

    We do not have specific angiogenesis-related categorization of the target for this array, but we do have some informative material available in our website:
    - https://www.bio-techne.com/research-area/angiogenesis
    - https://www.novusbio.com/research-areas/cancer/angiogenesis

  • Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?

    The proteinase inhibitors are recommended for cell and tissue lysates only.  You do not need them for cell culture supernates.

  • Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

  • Which gene of the VEGF family is detected on E19 and E20

    The VEGF spots are detecting VEGF-A.

  • Does the Human Angiogenesis Proteome Profiler Array cross-react with other species like equines?

    We have not tested this kit with equine samples. However, when looking at the cross reactivity with our Proteome Profiler Arrays, there are a few things to keep in mind. When the arrays are being designed, we look for the best antibodies that will work for the species stated. This does not mean there will not be any cross reactivity between species but it does suggest that the assay would not perform equally between species. While it is possible that some of the antibodies may detect the equine protein, different antibodies cross-react at different levels.

    It is also important to note that the Proteome Profiler Arrays are designed to be used as a screening tool. This may be a good place to start if you are trying determine what may be having an increase or a decrease in signal. We recommend running one untreated sample and comparing them to your treated samples. Even though you may not have the exact same cross reactivity with each analyte, you may be able to detect the increase or decrease from your samples.

    We have searched for citations that may have referenced using this kit with equine samples and were able to find 1. Here is the link to it: https://www.ncbi.nlm.nih.gov/pubmed/25313202.

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