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Proteome Profiler Human Angiogenesis Array Kit
R&D Systems, Inc. a Bio-Techne Brand
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Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software. |
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The Human Angiogenesis Array detects multiple analytes in cell culture supernates. A. THP-1 human acute monocytic leukemia cell line treated with 1 µg/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 8 hours and 1 µg/mL lipopolysaccharide (LPS) for 16 hours. B. HUVEC human umbilical vein endothelial cells treated with 10 mg/ml of LPS for 24 hours. 500 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
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The Human Angiogenesis Array detects multiple analytes in tissue lysate, serum, and plasma. Representative membranes and selective quantification are shown for human ovarian cancer tissue lysate (A., 200 µg), serum (B., 100 µL), and EDTA plasma (C., 100 µL). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System. |
Citations for Proteome Profiler Human Angiogenesis Array Kit (173)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Human Angiogenesis Array Kit include:
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Species: Human
Sample Types: Cell Culture Supernates
SP Meda Venka et al. (2023), Inhibition of GPR39 restores defects in endothelial cell-mediated neovascularization under the duress of chronic hyperglycemia: Evidence for regulatory roles of the sonic hedgehog signaling axis Proceedings of the National Academy of Sciences of the United States of America, 120(1):e2208541120.
PMID: 36574661 -
Species: Human
Sample Types: Cell Culture Supernates
SY Wei et al. (2022), Strategy for improving cell-mediated vascularized soft tissue formation in a hydrogen peroxide-triggered chemically-crosslinked hydrogel Journal of tissue engineering, 13(0):2041731422108.
PMID: 35296029 -
Species: Human
Sample Types: Cell Culture Supernates
Y Qin et al. (2022), PAI-1 is a vascular cell-specific HIF-2-dependent angiogenic factor that promotes retinal neovascularization in diabetic patients Science Advances, 8(9):eabm1896.
PMID: 35235351 -
Species: Human
Sample Types: Cell Culture Supernates
PM Tran et al. (2022), Staphylococcus aureus beta-Toxin Exerts Anti-angiogenic Effects by Inhibiting Re-endothelialization and Neovessel Formation Frontiers in Microbiology, 13(0):840236.
PMID: 35185854 -
Species: Human
Sample Types: Cell Lysates
M Aloysius D et al. (2022), High glucose induced inflammation is inhibited by copper chelation via rescuing mitochondrial fusion protein 2 in retinal pigment epithelial cells Cellular Signalling, 0(0):110244.
PMID: 34999205 -
Species: Human
Sample Types: Cell Culture Supernates
V Ormazabal et al. (2022), Secretome from Human Mesenchymal Stem Cells-Derived Endothelial Cells Promotes Wound Healing in a Type-2 Diabetes Mouse Model International Journal of Molecular Sciences, 23(2).
PMID: 35055129 -
Species: Human
Sample Types: Cell Lysates
IS Miller et al. (2022), Implementing subtype-specific pre-clinical models of breast cancer to study pre-treatment aspirin effects Cancer Medicine, 0(0).
PMID: 35434898 -
Species: Human
Sample Types: Cell Culture Supernates
Y Wei et al. (2022), A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer Cell Death Disease, 2022;13(4):294.
PMID: 35365616 -
Species: Human
Sample Types: Cell Lysates
I Marei et al. (2022), Angiogenic content of microparticles in patients with diabetes and coronary artery disease predicts networks of endothelial dysfunction Cardiovascular Diabetology, 21(1):17.
PMID: 35109843 -
Species: Human
Sample Types: Cell Culture Supernates
A Bugatti et al. (2022), SARS-CoV-2 Infects Human ACE2-Negative Endothelial Cells through an alphavbeta3 Integrin-Mediated Endocytosis Even in the Presence of Vaccine-Elicited Neutralizing Antibodies Viruses, 14(4).
PMID: 35458435 -
Species: Human
Sample Types: Cell Culture Supernates
M Ehsani et al. (2022), The natural compound atraric acid suppresses androgen-regulated neo-angiogenesis of castration-resistant prostate cancer through angiopoietin 2 Oncogene, 0(0).
PMID: 35513564 -
Species: Human
Sample Types: Cell Culture Supernates
Q Du et al. (2022), FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis Cell Death Disease, 2022;13(5):488.
PMID: 35597782 -
Species: Human
Sample Types: Cell Culture Supernates
BS Casas et al. (2022), Schizophrenia-derived hiPSC brain microvascular endothelial-like cells show impairments in angiogenesis and blood-brain barrier function Molecular Psychiatry, 0(0).
PMID: 35705634 -
Species: Human
Sample Types: Cell Culture Supernates
O Cases-Pere et al. (2022), Development of secretome-based strategies to improve cell culture protocols in tissue engineering Oncogene, 12(1):10003.
PMID: 35705659 -
Species: Human
Sample Types: Cell Culture Supernates
C Helissey et al. (2022), Two New Potential Therapeutic Approaches in Radiation Cystitis Derived from Mesenchymal Stem Cells: Extracellular Vesicles and Conditioned Medium Biology, 11(7).
PMID: 36101361 -
Species: Human
Sample Types: Cell Culture Supernates
S Freitas-Ri et al. (2022), Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue Marine Drugs, 20(10).
PMID: 36286447 -
Species: Human
Sample Types: Cell Lysates
OM David et al. (2022), The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells Biomolecules, 12(10).
PMID: 36291543 -
Species: Human
Sample Types: Cell Culture Supernates
AB Jaykumar et al. (2022), WNK1 collaborates with TGF-beta in endothelial cell junction turnover and angiogenesis Proceedings of the National Academy of Sciences of the United States of America, 119(30):e2203743119.
PMID: 35867836 -
Species: Human
Sample Types: Cell Lysates
Y Gao et al. (2022), MIIP functions as a novel ligand for ITGB3 to inhibit angiogenesis and tumorigenesis of triple-negative breast cancer Cell Death Disease, 2022;13(9):810.
PMID: 36130933 -
Species: Human
Sample Types: Cell Culture Supernates
J Chivot et al. (2022), PARP Inhibitor Inhibits the Vasculogenic Mimicry through a NF-kappaB-PTX3 Axis Signaling in Breast Cancer Cells International Journal of Molecular Sciences, 23(24).
PMID: 36555812 -
Species: Human
Sample Types: Cell Lysates
JMF Vieira et al. (2022), Overexpression of mir-135b and mir-210 in mesenchymal stromal cells for the enrichment of extracellular vesicles with angiogenic factors PLoS ONE, 17(8):e0272962.
PMID: 35972944 -
Species: Human
Sample Types: Cell Supernates
Y Zhang et al. (2021), Gold nanoparticles inhibit activation of cancer-associated fibroblasts by disrupting communication from tumor and microenvironmental cells Bioact Mater, 6(2):326-332.
PMID: 32954051 -
Species: Human
Sample Types: Cell Culture Supernates
N Komabashir et al. (2021), Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells Regenerative Therapy, 18(0):38-50.
PMID: 33869686 -
Species: Human
Sample Types: Cell Lysates
R Mabasa et al. (2021), Ricinus communis Butanol Fraction Inhibits MCF-7 Breast Cancer Cell Migration, Adhesion, and Invasiveness Integrative cancer therapies, 20(0):1534735420977.
PMID: 33565349 -
Species: Human
Sample Types: Cell Culture Supernates
J Javadi et al. (2021), Syndecan-1 Overexpressing Mesothelioma Cells Inhibit Proliferation, Wound Healing, and Tube Formation of Endothelial Cells Cancers, 13(4).
PMID: 33562126 -
Species: Human
Sample Types: Cell Culture Supernates
K Heinze et al. (2021), RUNX3 Transcript Variants Have Distinct Roles in Ovarian Carcinoma and Differently Influence Platinum Sensitivity and Angiogenesis Cancers, 13(3).
PMID: 33530588 -
Species: Human
Sample Types: Cell Culture Supernates
Z Cheng et al. (2021), Serum-Derived Small Extracellular Vesicles From Diabetic Mice Impair Angiogenic Property of Microvascular Endothelial Cells: Role of EZH2 Journal of the American Heart Association, 10(10):e019755.
PMID: 33988033 -
Species: Human
Sample Types: Cell Culture Supernates
AC Andrade et al. (2021), Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles International Journal of Molecular Sciences, 22(8).
PMID: 33918735 -
Species: Human
Sample Types: Cell Lysates
S Herbster et al. (2021), Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms Cancers, 13(9).
PMID: 34066355 -
Species: Human
Sample Types: Cell Culture Supernates
E Nassar et al. (2021), Syndecan-1 Promotes Angiogenesis in Triple-Negative Breast Cancer through the Prognostically Relevant Tissue Factor Pathway and Additional Angiogenic Routes Cancers, 13(10).
PMID: 34066023 -
Species: Human
Sample Types: Cell Culture Supernates
H Sheldon et al. (2021), ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype International Journal of Molecular Sciences, 22(20).
PMID: 34681953 -
Species: Human
Sample Types: Cell Lysates
S Ma et al. (2021), CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance Cell Reports, 36(7):109549.
PMID: 34407412 -
Species: Human
Sample Types: Cell Culture Supernates
F Caccuri et al. (2021), SARS-CoV-2 Infection Remodels the Phenotype and Promotes Angiogenesis of Primary Human Lung Endothelial Cells Microorganisms, 9(7).
PMID: 34361874 -
Species: Human
Sample Types: Cell Lysates
CY Hwang et al. (2021), Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor Cancer Gene Therapy, 0(0).
PMID: 34363028 -
Species: Human
Sample Types: Cell Culture Supernates
ZK Moay et al. (2021), Keratin-Alginate Sponges Support Healing of Partial-Thickness Burns International Journal of Molecular Sciences, 22(16).
PMID: 34445299 -
Species: Human
Sample Types: Cell Culture Supernates
Z Gong et al. (2021), Dexamethasone enhances the efficacy of atorvastatin in inhibiting excessively inflammation-induced abnormal angiogenesis by regulating macrophages Journal of Neuroinflammation, 18(1):203.
PMID: 34526068 -
Species: Human
Sample Types: Cell Culture Supernates
HW Huang et al. (2021), CMAHP promotes metastasis by reducing ubiquitination of Snail and inducing angiogenesis via GM-CSF overexpression in gastric cancer Oncogene, 0(0).
PMID: 34716430 -
Species: Mouse
Sample Types: Plasma
J Deng et al. (2021), Eicosanoid regulation of debris-stimulated metastasis Proceedings of the National Academy of Sciences of the United States of America, 118(41).
PMID: 34607951 -
Species: Human
Sample Types: Cell Culture Supernates
A Riley et al. (2021), Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device Oncology Letters, 22(5):780.
PMID: 34594421 -
Species: Human
Sample Types: Cell Culture Supernates
Y Asano et al. (2021), Construction of transplantable artificial vascular tissue based on adipose tissue-derived mesenchymal stromal cells by a cell coating and cryopreservation technique Scientific Reports, 11(1):17989.
PMID: 34504254 -
Species: Human
Sample Types: Cell Culture Supernates
SS Ha et al. (2021), Nano-Sized Extracellular Matrix Particles Lead to Therapeutic Improvement for Cutaneous Wound and Hindlimb Ischemia International Journal of Molecular Sciences, 22(24).
PMID: 34948061 -
Species: Human
Sample Types: Cell Culture Supernates
T Nishikai-Y et al. (2021), MMP9 secreted from mononuclear cell quality and quantity culture mediates STAT3 phosphorylation and fibroblast migration in wounds Regenerative Therapy, 18(0):464-471.
PMID: 34805452 -
Species: Human
Sample Types: Cell Lysates
T Shi et al. (2021), Evaluating the Effect of Lenvatinib on Sorafenib-Resistant Hepatocellular Carcinoma Cells International Journal of Molecular Sciences, 22(23).
PMID: 34884875 -
Species: Porcine
Sample Types: Tissue Homogenates
SH Kim et al. (2021), The Regeneration of Large-Sized and Vascularized Adipose Tissue Using a Tailored Elastic Scaffold and dECM Hydrogels International Journal of Molecular Sciences, 22(22).
PMID: 34830444 -
Species: Human
Sample Types: Cell Lysates
YJ Kim et al. (2021), Lightwave-reinforced stem cells with enhanced wound healing efficacy Journal of tissue engineering, 12(0):2041731421106.
PMID: 34987748 -
Species: Human
Sample Types: Cell Lysate
CT Su et al. (2021), LTBP4 affects renal fibrosis by influencing angiogenesis and altering mitochondrial structure Cell Death Disease, 2021;12(10):943.
PMID: 34645813 -
Species: Human
Sample Types: Cell Culture Supernates
E Varone et al. (2021), The ER stress response mediator ERO1 triggers cancer metastasis by favoring the angiogenic switch in hypoxic conditions Oncogene, 0(0).
PMID: 33531624 -
Species: Human
Sample Types: Cell Lysates
SH Qin et al. (2020), Resveratrol Promotes Tumor Microvessel Growth via Endoglin and Extracellular Signal-Regulated Kinase Signaling Pathway and Enhances the Anticancer Efficacy of Gemcitabine against Lung Cancer Cancers (Basel), 12(4).
PMID: 32326402 -
Species: Human
Sample Types: Cell Lysates
AG Kutikhin et al. (2020), Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature Cells, 9(4).
PMID: 32260159 -
Species: Human
Sample Types: Cell Culture Supernates
JW Lim et al. (2020), An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding Polymers (Basel), 12(3).
PMID: 32150989 -
Species: Human
Sample Types: Cell Culture Supernates
TM Pausch et al. (2020), Metastasis-associated fibroblasts promote angiogenesis in metastasized pancreatic cancer via the CXCL8 and the CCL2 axes Sci Rep, 10(1):5420.
PMID: 32214219 -
Species: Human
Sample Types: Cell Culture Supernates
A Ratushnyy et al. (2020), Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis Int J Mol Sci, 21(5).
PMID: 32151085 -
Species: Human
Sample Types: Cell Culture Lysates
SJ Szymura et al. (2020), DDX39B interacts with the pattern recognition receptor pathway to inhibit NF-&kappaB and sensitize to alkylating chemotherapy BMC Biol., 18(1):32.
PMID: 32209106 -
Species: Human
Sample Types: Cell Culture Supernates
P Muniyandi et al. (2020), ECM Mimetic Electrospun Porous Poly (L-lactic acid) (PLLA) Scaffolds as Potential Substrates for Cardiac Tissue Engineering Polymers (Basel), 12(2).
PMID: 32075089 -
Species: Human
Sample Types: Cell Culture Lysates
M Leite et al. (2020), Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions Cells, 9(2).
PMID: 32102381 -
Species: Human
Sample Types: Cell Culture Supernates
JJ Gu et al. (2020), Mesenchymal stem cells promote metastasis through activation of an ABL-MMP9 signaling axis in lung cancer cells PLoS ONE, 15(10):e0241423.
PMID: 33119681 -
Species: Human
Sample Types: Tissue Homogenates
K Hess et al. (2020), Occurrence of Fibrotic Tumor Vessels in Grade I Meningiomas Is Strongly Associated with Vessel Density, Expression of VEGF, PlGF, IGFBP-3 and Tumor Recurrence Cancers (Basel), 12(10).
PMID: 33096816 -
Species: Human
Sample Types: MEM
H Brandhorst et al. (2020), Proteomic Profiling Reveals the Ambivalent Character of the Mesenchymal Stem Cell Secretome: Assessing the Effect of Preconditioned Media on Isolated Human Islets Cell Transplant, 29(0):9636897209523.
PMID: 33150790 -
Species: Human
Sample Types: Cell Culture Supernates
TJ Kwak et al. (2020), In vitro modeling of solid tumor interactions with perfused blood vessels Sci Rep, 10(1):20142.
PMID: 33214583 -
Species: Human
Sample Types: Cell Culture Supernates
JE Chen et al. (2020), Crosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model J Neuroinflammation, 17(1):346.
PMID: 33208156 -
Species: Human
Sample Types: Cell Lysates
X Li et al. (2020), IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 FEBS Open Bio, 0(0).
PMID: 33064893 -
Species: Human
Sample Types: Cell Culture Supernates
L Rao et al. (2020), HB-EGF-EGFR Signaling in Bone Marrow Endothelial Cells Mediates Angiogenesis Associated with Multiple Myeloma Cancers (Basel), 12(1).
PMID: 31936715 -
Species: Human
Sample Types: Cell Culture Supernates
K Menezes et al. (2020), Human mesenchymal stromal/stem cells recruit resident pericytes and induce blood vessels maturation to repair experimental spinal cord injury in rats Sci Rep, 10(1):19604.
PMID: 33177535 -
Species: Human
Sample Types: Cell Culture Supernates
M Vijayan et al. (2020), Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7 J. Cell. Sci., 0(0).
PMID: 32513821 -
Species: Human
Sample Types: Serum
D Kardos et al. (2019), The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method Int J Mol Sci, 20(3).
PMID: 30743992 -
Species: Human
Sample Types: Cell Lysates
I Saltarella et al. (2019), Homotypic and Heterotypic Activation of the Notch Pathway in Multiple Myeloma-Enhanced Angiogenesis: A Novel Therapeutic Target? Neoplasia, 21(1):93-105.
PMID: 30529074 -
Species: Human
Sample Types: Cell Lysates
DK Sung et al. (2019), Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content J Clin Med, 8(4).
PMID: 31003433 -
Species: Human
Sample Types: Cell Culture Supernates
L Hummitzsch et al. (2019), Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after Ischemia/Reperfusion Injury In Vitro Stem Cells Int, 2019(0):3725863.
PMID: 31341483 -
Species: Human
Sample Types: Cell Culture Supernates
G Xiong et al. (2019), Impaired Regeneration Potential in Urinary Stem Cells Diagnosed from the Patients with Diabetic Nephropathy Theranostics, 9(14):4221-4232.
PMID: 31281543 -
Species: Human
Sample Types: Cell Culture Supernates
EE Hedlund et al. (2019), Harnessing Induced Essentiality: Targeting Carbonic Anhydrase IX and Angiogenesis Reduces Lung Metastasis of Triple Negative Breast Cancer Xenografts Cancers (Basel), 11(7).
PMID: 31319613 -
Species: Human
Sample Types: Whole Cells
T Matsui et al. (2019), Telmisartan Inhibits Cell Proliferation and Tumor Growth of Esophageal Squamous Cell Carcinoma by Inducing S-Phase Arrest In Vitro and In Vivo Int J Mol Sci, 20(13).
PMID: 31261874 -
Species: Human
Sample Types: Urine
K Lang et al. (2019), TGFBI Protein Is Increased in the Urine of Patients with High-Grade Urothelial Carcinomas, and Promotes Cell Proliferation and Migration Int J Mol Sci, 20(18).
PMID: 31514337 -
Species: Human
Sample Types: Cell Culture Supernates
M Di Somma et al. (2019), Natural Histogel-Based Bio-Scaffolds for Sustaining Angiogenesis in Beige Adipose Tissue Cells, 8(11).
PMID: 31752157 -
Species: Human
Sample Types: Cell Culture Supernates
KE Lee et al. (2019), The efficacy of conditioned medium released by tonsil-derived mesenchymal stem cells in a chronic murine colitis model PLoS ONE, 14(12):e0225739.
PMID: 31790467 -
Species: Human
Sample Types: Cell Lysates
C Gao et al. (2019), Hematoma-derived exosomes of chronic subdural hematoma promote abnormal angiogenesis and inhibit hematoma absorption through miR-144-5p Aging (Albany NY), 11(24):12147-12164.
PMID: 31841443 -
Species: Human
Sample Types: Cell Culture Supernates
CH Chou et al. (2019), B-Cell Activating Factor Enhances Hepatocyte-Driven Angiogenesis via B-Cell CLL/Lymphoma 10/Nuclear Factor-KappaB Signaling during Liver Regeneration Int J Mol Sci, 20(20).
PMID: 31658764 -
Species: Human
Sample Types: Cell Culture Supernates
J Kelleher et al. (2019), Patient-Specific iPSC Model of a Genetic Vascular Dementia Syndrome Reveals Failure of Mural Cells to Stabilize Capillary Structures Stem Cell Reports, 13(5):817-831.
PMID: 31680059 -
Species: Human
Sample Types: Extracellular Vesicle Lysate
F Perut et al. (2019), Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis Cancers (Basel), 11(6).
PMID: 31195680 -
Species: Human
Sample Types: Cell Lysates
KP O'Brien et al. (2018), Employing mesenchymal stem cells to support tumor-targeted delivery of extracellular vesicle (EV)-encapsulated microRNA-379 Oncogene, 0(0).
PMID: 29367765 -
Species: Human
Sample Types: Cell Lysates
DT Dao et al. (2018), Intranasal delivery of VEGF enhances compensatory lung growth in mice PLoS ONE, 13(6):e0198700.
PMID: 29879188 -
Species: Human
Sample Types: Cell Culture Supernates
SL Lin et al. (2018), Stimulation of Interferon-Stimulated Gene 20 by Thyroid Hormone Enhances Angiogenesis in Liver Cancer Neoplasia, 20(1):57-68.
PMID: 29195126 -
Species: Human
Sample Types: Cell Lysates
CM Galmarini et al. (2018), Plocabulin, a novel tubulin-binding agent, inhibits angiogenesis by modulation of microtubule dynamics in endothelial cells BMC Cancer, 18(1):164.
PMID: 29415678 -
Species: Human
Sample Types: Cell Culture Supernates
MA Gharaei et al. (2018), Human dental pulp stromal cell conditioned medium alters endothelial cell behavior Stem Cell Res Ther, 9(1):69.
PMID: 29562913 -
Species: Human
Sample Types: Follicular Fluid
CC Huang et al. (2018), Increased platelet factor 4 and aberrant permeability of follicular fluid in PCOS J. Formos. Med. Assoc., 0(0).
PMID: 29779926 -
Species: Human
Sample Types: Tissue Homogenates
RF da Silva et al. (2018), Nintedanib inhibits growth of human prostate carcinoma cells by modulating both cell cycle and angiogenesis regulators Sci Rep, 8(1):9540.
PMID: 29934570 -
Species: Human
Sample Types: Cell Culture Supernates
M Beerens et al. (2018), Multipotent Adult Progenitor Cells Support Lymphatic Regeneration at Multiple Anatomical Levels during Wound Healing and Lymphedema Sci Rep, 8(1):3852.
PMID: 29497054 -
Species: Human
Sample Types: Cell Culture Supernates
HK Kim et al. (2018), A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells Stem Cells, 0(0).
PMID: 30281870 -
Species: Human
Sample Types: Cell Culture Supernates
L Rao et al. (2018), Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin� protein MP0250: a preclinical study Oncotarget, 9(17):13366-13381.
PMID: 29568363 -
Species: Human
Sample Types: Cell Lysates
EMM Abdelwahab et al. (2018), Mitochondrial dysfunction is a key determinant of the rare disease lymphangioleiomyomatosis and provides a novel therapeutic target Oncogene, 0(0).
PMID: 30573768 -
Species: Human
Sample Types: Cell Lysates
A Kim et al. (2018), Isoliquiritin Apioside Suppresses in vitro Invasiveness and Angiogenesis of Cancer Cells and Endothelial Cells Front Pharmacol, 9(0):1455.
PMID: 30618749 -
Species: Human
Sample Types: Serum
H Hagman et al. (2018), Protein array profiling of circulating angiogenesis-related factors during bevacizumab containing treatment in metastatic colorectal cancer PLoS ONE, 13(12):e0209838.
PMID: 30592740 -
Species: Human
Sample Types: Cell Culture Supernates
BS Casas et al. (2018), hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis Transl Psychiatry, 8(1):48.
PMID: 29467462 -
Species: Human
Sample Types: Cell Culture Supernates
G Biondani et al. (2018), Extracellular Matrix composition modulates PDAC parenchymal and stem cell plasticity and behavior through the secretome FEBS J., 0(0).
PMID: 29660229 -
Species: Human
Sample Types: Cell Culture Supernates
JUG Wagner et al. (2018), Switch in Laminin β2 to Laminin β1 Isoforms During Aging Controls Endothelial Cell Functions Arterioscler. Thromb. Vasc. Biol., 0(0).
PMID: 29599141 -
Species: Human
Sample Types: Cell Culture Supernates
RJ Chen et al. (2017), Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 8(37):62081-62098.
PMID: 28977928 -
Species: Human
Sample Types: Cell Culture Supernates
A Osborne et al. (2017), Neuroprotective Effects of Human Mesenchymal Stem Cells and Platelet-Derived Growth Factor on Human Retinal Ganglion Cells Stem Cells, 0(0).
PMID: 29044808 -
Species: Human
Sample Types: Cell Culture Supernates
G Zavala et al. (2017), Sonic hedgehog (SHH) signaling improves the angiogenic potential of Wharton's jelly-derived mesenchymal stem cells�(WJ-MSC) Stem Cell Res Ther, 8(1):203.
PMID: 28962669 -
Species: Human
Sample Types: Cell Culture Supernates
R Bibes et al. (2017), SCO-spondin oligopeptide inhibits angiogenesis in glioblastoma Oncotarget, 8(49):85969-85983.
PMID: 29156770 -
Species: Human
Sample Types: Cell Lysates
SS Kim et al. (2017), Targeted nanocomplex carrying siRNA against MALAT1 sensitizes glioblastoma to temozolomide Nucleic Acids Res., 0(0).
PMID: 29202181 -
Species: Human
Sample Types: Cell Culture Supernates
S Sakimoto et al. (2017), CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect JCI Insight, 2(2):e89906.
PMID: 28138561 -
Species: Human
Sample Types: Cell Culture Supernates
S Yang et al. (2017), NF-?B Signaling Activation Induced by Chloroquine Requires Autophagosome, p62 Protein, and c-Jun N-terminal Kinase (JNK) Signaling and Promotes Tumor Cell Resistance J. Biol. Chem., 292(8):3379-3388.
PMID: 28082672 -
Species: Human
Sample Types: Cell Lysates
HR Jung et al. (2017), Cell Spheroids with Enhanced Aggressiveness to Mimic Human Liver Cancer In Vitro and In Vivo Sci Rep, 7(1):10499.
PMID: 28874716 -
Species: Human
Sample Types: Cell Culture Supernates
L Qiang et al. (2017), Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 0(0):0.
PMID: 28933598 -
Species: Human
Sample Types: Cell Culture Supernates
A Gabrielyan et al. (2017), Metabolically conditioned media derived from bone marrow stromal cells or human skin fibroblasts act as effective chemoattractants for mesenchymal stem cells Stem Cell Res Ther, 8(1):212.
PMID: 28969687 -
Species: Human
Sample Types: Cell Culture Supernates
G Jin et al. (2017), Combination curcumin and (-)-epigallocatechin-3-gallate inhibits colorectal carcinoma microenvironment-induced angiogenesis by JAK/STAT3/IL-8 pathway Oncogenesis, 6(10):e384.
PMID: 28967875 -
Species: Human
Sample Types: Cell Lysates
R El Bezawy et al. (2017), Antitumor activity of miR-34a in peritoneal mesothelioma relies on c-MET and AXL inhibition: persistent activation of ERK and AKT signaling as a possible cytoprotective mechanism J Hematol Oncol, 10(1):19.
PMID: 28100259 -
Species: Human
Sample Types: Cell Lysates
F Reggiani et al. (2017), Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 0(0).
PMID: 28754674 -
Species: Human
Sample Types: Cell Lysates
S Tunçer et al. (2017), 15-Lipoxygenase-1 re-expression in colorectal cancer alters endothelial cell features through enhanced expression of TSP-1 and ICAM-1 Cell. Signal., 39(0):44-54.
PMID: 28757355 -
Species: Human
Sample Types: Tissue Homogenates
Y Wang et al. (2017), Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis Stem Cells, 0(0).
PMID: 28514506 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential Sci Rep, 6(0):36277.
PMID: 27808120 -
Species: Human
Sample Types: Cell Lysates
(2016), Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment PLoS ONE, 11(7):e0159013.
PMID: 27391808 -
Species: Human
Sample Types: Cell Culture Supernates
M Boufraqech et al. (2016), Lysyl Oxidase (LOX) transcriptionally regulates SNAI2 expression and TIMP4 secretion in human cancers Clin Cancer Res, 0(0).
PMID: 27029493 -
Species: Human
Sample Types: Cell Culture Supernates
Gabriela Odent Grig (2016), Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 0(0).
PMID: 27943613 -
Species: Human
Sample Types: Cell Culture Supernates
Matthew N Svalina (2016), IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma Sci Rep, 6(0):27012.
PMID: 27255663 -
Species: Human
Sample Types: Tumor Interstitial Fluid
(2016), The Angiogenic Secretome in VEGF overexpressing Breast Cancer Xenografts Sci Rep, 6(0):39460.
PMID: 27995973 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), Hypoxia-induced responses by endothelial colony-forming cells are modulated by placental growth factor Stem Cell Res Ther, 7(1):173.
PMID: 27899144 -
Species: Human
Sample Types: Cell Culture Supernates
(2016), A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery PLoS ONE, 11(11):e0166639.
PMID: 27846318 -
Species: Human
Sample Types: Ascites Fluid
Sofia-Paraskevi Trachana (2016), The Development of an Angiogenic Protein Signature in Ovarian Cancer Ascites as a Tool for Biologic and Prognostic Profiling PLoS ONE, 11(6):e0156403.
PMID: 27258020 -
Species: Human
Sample Types: Cell Culture Supernates
Y Deng et al. (2016), Prostacyclin-producing human mesenchymal cells target H19 lncRNA to augment endogenous progenitor function in hindlimb ischaemia Nat Commun, 7(0):11276.
PMID: 27080438 -
Species: Human
Sample Types: Cell Culture Supernates
Chun-Wun Lu (2016), VLDL and LDL, but not HDL, promote breast cancer cell proliferation, metastasis and angiogenesis Cancer Lett, 388(0):130-138.
PMID: 27940127 -
Species: Porcine
Sample Types: Cell Culture Supernates
Christian Freese (2016), Identification of neuronal and angiogenic growth factors in an in vitroblood-brain barrier model system: Relevance in barrier integrity and tight junction formation and complexity Microvasc. Res, 0(0).
PMID: 27988246 -
Species: Human
Sample Types: Cell Culture Supernates
Ukaji T et al. (2015), Inhibition of IGF-1-Mediated Cellular Migration and Invasion by Migracin A in Ovarian Clear Cell Carcinoma Cells. PLoS ONE, 10(9):e0137663.
PMID: 26360832 -
Species: Human
Sample Types: Cell Culture Supernates
Walsh et al. (2015), RECK controls breast cancer metastasis by modulating a convergent, STAT3-dependent neoangiogenic switch. Oncogene, 34(17):2189-203.
PMID: 24931164 -
Species: Human
Sample Types: Cell Lysates
Paggetti J et al. (2015), Exosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts. Blood, 126(9):1106-17.
PMID: 26100252 -
Species: Human
Sample Types: Cell Culture Supernates
Spampinato S et al. (2015), Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli? PLoS ONE, 10(7):e0133392.
PMID: 26197437 -
Species: Human
Sample Types: Cell Culture Supernates
Mazumdar et al. (2015), CoREST1 promotes tumor formation and tumor stroma interactions in a mouse model of breast cancer. PLoS ONE, 10(3):e0121281.
PMID: 25793264 -
Species: Human
Sample Types: Cell Culture Supernates
Merkely B et al. (2015), Signaling via PI3K/FOXO1A pathway modulates formation and survival of human embryonic stem cell-derived endothelial cells. Stem Cells Dev, 24(7):869-78.
PMID: 25387407 -
Species: Human
Sample Types: Cell Culture Supernates
Blocki A et al. (2015), Sourcing of an alternative pericyte-like cell type from peripheral blood in clinically relevant numbers for therapeutic angiogenic applications. Mol Ther, 23(3):510-22.
PMID: 25582709 -
Species: Human
Sample Types: Cell Culture Supernates
Gustafson M et al. (2015), Intratumoral CD14+ Cells and Circulating CD14+HLA-DRlo/neg Monocytes Correlate with Decreased Survival in Patients with Clear Cell Renal Cell Carcinoma. Clin Cancer Res, 21(18):4224-33.
PMID: 25999436 -
Species: Human
Sample Types: Cell Lysates
Lee S et al. (2015), Deficiency of endothelium-specific transcription factor Sox17 induces intracranial aneurysm. , 0(0).
PMID: 25596186 -
Species: Human
Sample Types: Cell Lysates
Chung S et al. (2015), Identification of Novel Biomarkers for Metastatic Colorectal Cancer Using Angiogenesis-Antibody Array and Intracellular Signaling Array. PLoS ONE, 10(8):e0134948.
PMID: 26258407 -
Species: Human
Sample Types: Cell Culture Supernates
Ball S et al. (2014), Mesenchymal stromal cells: inhibiting PDGF receptors or depleting fibronectin induces mesodermal progenitors with endothelial potential. Stem Cells, 32(3):694-705.
PMID: 24022915 -
Species: Equine
Sample Types: Cell Culture Supernates
Bussche L et al. (2014), Peripheral Blood-Derived Mesenchymal Stromal Cells Promote Angiogenesis via Paracrine Stimulation of Vascular Endothelial Growth Factor Secretion in the Equine Model. Stem Cells Transl Med, 3(12):1514-25.
PMID: 25313202 -
Species: Human
Sample Types: Cell Culture Supernates
Santhekadur P et al. (2014), Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFbeta signaling. FEBS Open Bio, 4(0):353-61.
PMID: 24918049 -
Species: Human
Sample Types: Cell Culture Supernates
Ruma I et al. (2014), Extract of Cordyceps militaris inhibits angiogenesis and suppresses tumor growth of human malignant melanoma cells. Int J Oncol, 45(1):209-18.
PMID: 24789042 -
Species: Human
Sample Types: Cell Culture Supernates
Lee E et al. (2014), Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nat Commun, 5(0):4715.
PMID: 25178650 -
Species: Human
Sample Types: Cell Lysates
Reckhenrich A et al. (2014), Surgical sutures filled with adipose-derived stem cells promote wound healing. PLoS ONE, 9(3):e91169.
PMID: 24625821 -
Lee et al. (2014), Lymphatic endothelial cells support tumor growth in breast cancer. Sci Rep, 4(0):5853.
PMID: 25068296 -
Species: Mouse
Sample Types: Serum
Eom S et al. (2014), Histone deacetylase-3 mediates positive feedback relationship between anaphylaxis and tumor metastasis. J Biol Chem, 289(17):12126-44.
PMID: 24619412 -
Hutzen et al. (2014), Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin. BMC Cancer, 14(0):206.
PMID: 24646176 -
Species: Human
Sample Types: Cell Lysates
Wilson C et al. (2014), Sortilin mediates the release and transfer of exosomes in concert with two tyrosine kinase receptors. J Cell Sci, 127(0):3983-97.
PMID: 25037567 -
Species: Human
Sample Types: Cell Culture Supernates
Shen C et al. (2014), Ionizing radiation induces tumor cell lysyl oxidase secretion. BMC Cancer, 14(0):532.
PMID: 25052686 -
Species: Human
Sample Types: Whole Cells
Wojciak-Stothard B et al. (2014), Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension. Circulation, 129(17):1770-80.
PMID: 24503951 -
Species: Human
Sample Types: Cell Culture Supernates
Chuang et al. (2014), Lysophosphatidic acid enhanced the angiogenic capability of human chondrocytes by regulating Gi/NF-kB-dependent angiogenic factor expression. PLoS ONE, 9(5):e95180.
PMID: 24879414 -
Species: Human
Sample Types: Cell Culture Supernates
Kryza T et al. (2014), Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway. FASEB J, 28(2):740-51.
PMID: 24225148 -
Species: Human
Sample Types: Cell Culture Supernates
Mriouah J et al. (2014), PTEN expression is involved in the invasive properties of HNSCC: a key protein to consider in locoregional recurrence. Int J Oncol, 44(3):709-16.
PMID: 24366426 -
Species: Human
Sample Types: Cell Lysates
(2013), Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species. Stem Cells Dev, 22(1):158-68.
PMID: 22784094 -
Species: Human
Sample Types: Cell Lysates
Box C et al. (2013), A novel serum protein signature associated with resistance to epidermal growth factor receptor tyrosine kinase inhibitors in head and neck squamous cell carcinoma. Eur J Cancer, 49(0):2512-21.
PMID: 23582742 -
Species: Human
Sample Types: Cell Lysates
(2013), Inhibition of TGF-β signaling by the fungal lactones (S)-curvularin, dehydrocurvularin, oxacyclododecindione and galiellalactone. Cytokine, 61(1):285-96.
PMID: 23134667 -
Species: Human
Sample Types: Cell Culture Supernates
Vereb Z et al. (2013), Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy. Biomed Res Int, 2013(0):492376.
PMID: 24195074 -
Species: Human
Sample Types: Cell Culture Supernates
Abuhusain et al. (2013), A metabolic shift favoring sphingosine 1-phosphate at the expense of ceramide controls glioblastoma angiogenesis. J Biol Chem, 288(52):37355-64.
PMID: 24265321 -
Species: Human
Sample Types: Cell Culture Supernates
Shamis Y et al. (2013), Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo. PLoS ONE, 8(12):e83755.
PMID: 24386271 -
Species: Human
Sample Types: Cell Culture Supernates
Porter et al. (2012), Cyclin-dependent kinase 8 mediates chemotherapy-induced tumor-promoting paracrine activities. Proc Natl Acad Sci U S A, 109(34):13799-804.
PMID: 22869755 -
Species: Human
Sample Types: Cell Culture Supernates
Sceneay J et al. (2012), Primary tumor hypoxia recruits CD11b+/Ly6Cmed/Ly6G+ immune suppressor cells and compromises NK cell cytotoxicity in the premetastatic niche. Cancer Res, 72(16):3906-11.
PMID: 22751463 -
Species: Human
Sample Types: Cell Culture Supernates
Benayoun L et al. (2012), Tumor-initiating cells of various tumor types exhibit differential angiogenic properties and react differently to antiangiogenic drugs. Stem Cells, 30(9):1831-41.
PMID: 22782858 -
Species: Human
Sample Types: Cell Culture Supernates
Santhekadur PK et al. (2012), Multifunction protein staphylococcal nuclease domain containing 1 (SND1) promotes tumor angiogenesis in human hepatocellular carcinoma through novel pathway that involves nuclear factor κB and miR-221. J. Biol. Chem., 287(17):13952-8.
PMID: 22396537 -
Species: Human
Sample Types: Cell Culture Supernates
(2012), Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A. Am. J. Pathol., 181(2):693-705.
PMID: 22659470 -
Species: Human
Sample Types: Cell Culture Supernates
Muffley L et al. (2012), Differentiation state determines neural effects on microvascular endothelial cells. Exp Cell Res, 318(16):2085-93.
PMID: 22683922 -
Species: Human
Sample Types: Cell Lysates
Heinke J et al. (2012), Bone morphogenetic protein modulator BMPER is highly expressed in malignant tumors and controls invasive cell behavior. Oncogene, 31(24):2919-30.
PMID: 22020334 -
Species: Human
Sample Types: Cell Lysates
(2012), Tumour-secreted miR-9 promotes endothelial cell migration and angiogenesis by activating the JAK-STAT pathway. EMBO J., 31(17):3513-23.
PMID: 22773185 -
Species: Human
Sample Types: Cell Culture Supernates
Hill KS et al. (2012), Met Receptor Tyrosine Kinase Signaling Induces Secretion of the Angiogenic Chemokine Interleukin-8/CXCL8 in Pancreatic Cancer. PLoS ONE, 7(7):e40420.
PMID: 22815748 -
Species: Human
Sample Types: Cell Culture Supernates
Rittig K et al. (2012), The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells. Diabetologia, 55(5):1514-25.
PMID: 22327346 -
Species: Human
Sample Types: Cell Culture Supernates
Iram N et al. (2012), Age-related changes in expression and function of Toll-like receptors in human skin. Development, 139(22):4210-9.
PMID: 23034637 -
Species: Human
Sample Types: Cell Culture Supernates
Santhekadur PK et al. (2012), Late SV40 factor (LSF) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP-9). J. Biol. Chem., 287(5):3425-32.
PMID: 22167195 -
Species: Human
Sample Types: Cell Lysates
Dormoy V et al. (2011), LIM-class homeobox gene Lim1, a novel oncogene in human renal cell carcinoma. Oncogene, 30(15):1753-63.
PMID: 21132009 -
Species: Human
Sample Types: Cell Culture Supernates
Bordoli MR et al. (2011), Prolyl-4-hydroxylase PHD2- and hypoxia-inducible factor 2-dependent regulation of amphiregulin contributes to breast tumorigenesis. Oncogene, 30(5):548-60.
PMID: 20856199 -
Species: Human
Sample Types: Cell Culture Supernates
Bitsika V et al. (2011), Human amniotic fluid-derived mesenchymal stem cells as therapeutic vehicles: a novel approach for the treatment of bladder cancer. Stem Cells Dev., 21(7):1097-111.
PMID: 21988169 -
Species: Human
Sample Types: Tissue Homogenates
Korampalli TS et al. (2011), Protein profiling of angiogenesis-related growth factors in laryngeal carcinoma: Pattern of protein expression in relation to tumour progression. Int. J. Oncol., 39(4):1033-9.
PMID: 21743965 -
Species: Human
Sample Types: Tissue Homogenates
Kachgal S et al. (2011), Mesenchymal stem cells from adipose and bone marrow promote angiogenesis via distinct cytokine and protease expression mechanisms. Angiogenesis, 14(1):47-59.
PMID: 21104120 -
Species: Human
Sample Types: Cell Culture Supernates
Miyanishi N et al. (2010), Involvement of autocrine CXCL12/CXCR4 system in the regulation of ovarian carcinoma cell invasion. Biochem. Biophys. Res. Commun., 403(1):154-9.
PMID: 21059341 -
Species: Human
Sample Types: Cell Culture Supernates
Baston-Bust DM et al. (2010), Syndecan-1 knock-down in decidualized human endometrial stromal cells leads to significant changes in cytokine and angiogenic factor expression patterns. Reprod. Biol. Endocrinol., 8(0):133.
PMID: 21044331 -
Species: Human
Sample Types: Cell Lysates
Gaiser T et al. (2009), p53-mediated inhibition of angiogenesis in diffuse low-grade astrocytomas. Neurochem. Int., 54(7):458-63.
PMID: 19428789 -
Species: Human
Sample Types: Cell Culture Supernates
Kanda T et al. (2009), MBP-1 inhibits breast cancer growth and metastasis in immunocompetent mice. Cancer Res., 69(0):9354-9.
PMID: 19934312
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Customer Reviews for Proteome Profiler Human Angiogenesis Array Kit (9)
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Verified Customer | Posted 11/21/2022HepG2 lysate concentrated, DMEM FBS free, 300 ug loaded.
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Verified Customer | Posted 02/07/2019
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Verified Customer | Posted 02/06/2019Worked well to look at THP-1 angiogenesis factors.
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Verified Customer | Posted 10/22/2018It worked well in cell culture supernatants using 125 ug of total protein per membrane.
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Verified Customer | Posted 08/08/2018
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Verified Customer | Posted 05/17/2018Used it on Conditioned medium collected from Breast Cancer Cells treated with Angiotensin II to look into its effect on Angiogenesis. Angiogenesis array worked really well and very easy to use. It provided some new promising gene targets which we did not know before.
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Verified Customer | Posted 04/01/2018
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Verified Customer | Posted 10/30/2017
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Verified Customer | Posted 12/06/2016Human Angiogenesis Antibody Array was used to evaluate mesenchymal stem cells secretion profiles when seeded in various extracellular matrices. Great tool that ganarates lots of data!
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FAQs for Proteome Profiler Human Angiogenesis Array Kit
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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What are the associated pathways each factor is involved in?
We do not have specific angiogenesis-related categorization of the target for this array, but we do have some informative material available in our website:
- https://www.bio-techne.com/research-area/angiogenesis
- https://www.novusbio.com/research-areas/cancer/angiogenesis -
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?
We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.
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Which gene of the VEGF family is detected on E19 and E20
The VEGF spots are detecting VEGF-A.
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Does the Human Angiogenesis Proteome Profiler Array cross-react with other species like equines?
We have not tested this kit with equine samples. However, when looking at the cross reactivity with our Proteome Profiler Arrays, there are a few things to keep in mind. When the arrays are being designed, we look for the best antibodies that will work for the species stated. This does not mean there will not be any cross reactivity between species but it does suggest that the assay would not perform equally between species. While it is possible that some of the antibodies may detect the equine protein, different antibodies cross-react at different levels.
It is also important to note that the Proteome Profiler Arrays are designed to be used as a screening tool. This may be a good place to start if you are trying determine what may be having an increase or a decrease in signal. We recommend running one untreated sample and comparing them to your treated samples. Even though you may not have the exact same cross reactivity with each analyte, you may be able to detect the increase or decrease from your samples.
We have searched for citations that may have referenced using this kit with equine samples and were able to find 1. Here is the link to it: https://www.ncbi.nlm.nih.gov/pubmed/25313202.
