Human IL-8/CXCL8 Quantikine HS ELISA Kit

Name: Human IL-8/CXCL8 Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HS800

R&D Systems, Inc. a Bio-Techne Brand

Catalog # HS800

Citations (31)

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sample Types:	Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.4 pg/mL
Assay Range:	1 - 64 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Human IL-8/CXCL8 Quantikine HS ELISA Kit Summary:	The Quantikine HS Human IL-8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-8 levels in serum and plasma. It contains E. coli-expressed recombinant human IL-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-8 accurately. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

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Product Specifications for Human IL-8/CXCL8 Quantikine HS ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-8
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	5.5	18.7	37.1	5	19.4	39.2
Standard Deviation	0.3	0.7	2.7	0.4	1.6	3.7
CV%	5.5	3.7	7.3	8	8.2	9.4

Recovery

The recovery of IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	95	86-102
Heparin Plasma (n=4)	99	91-107
Serum (n=4)	99	85-110

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IL-8/CXCL8 Quantikine HS ELISA Kit

	Human CXCL8/IL-8 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as IL-8, GCP-1, and NAP-1, is a heparin-binding 8-9 kDa member of the alpha, or CXC family of chemokines. There are at least 15 human CXC family members that all adopt a three beta -sheet/one alpha -helix structure. Most CXC chemokines show an N-terminal Glu-Leu-Arg (ELR) tripeptide motif. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is considered the most bio-active, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). Mature human IL-8 shares 65-69% amino acid (aa) identity with canine, feline, and porcine IL-8. There is no IL-8 gene counterpart in rodent.

Multiple isoforms of IL-8 are generated through both alternative splicing and differential proteolytic cleavage. In humans, alternative splicing generates an iso-form with an eleven aa substitution at the C-terminus. Proteolytic processing results in N-terminal truncation of IL-8 and is likely a cell-specific event. For example, fibroblasts and endothelial cells generate the 1-77 form by cleaving IL-8 following Glu21, while monocytes and lymphocytes generate the 6-77 form by cleaving following Leu25. These truncated forms generally show increased bioactivity, particularly through the CXCR1 receptor. IL-8 can also undergo citrullination on Arg27 of the precursor, a modification that increases its half-life and ability to induce leukocytosis. A wide variety of cells secrete IL-8 including monocytes and neutrophils, fibroblasts and keratinocytes, mast cells, visceral smooth muscle cells, dendritic cells, type II great alveolar cells, and endothelial cells.

IL-8 bioactivity is mediated through two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR1 is 45-50 kDa in size and is used almost exclusively by IL-8. CXCR2 is 35-40 kDa in size and is used by nearly all CXC chemokines. Both CXCR1 and CXCR2 constitutively associate into functional homodimers. They can also heterodimerize, but these complexes dissociate following IL-8 binding. CXCR2 responds to low concentrations of IL-8 and is principally associated with chemotaxis and MMP-9 release. CXCR1, in contrast, responds to high concentrations of IL-8 and is associated with respiratory burst and phospholipase D2 activation. Thus, CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. IL-8 can also form a complex with Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8 interaction with CXCR1.

In addition to its pro-inflammatory effects, IL-8 is involved in angiogenesis and the pathogenesis of atherosclerosis and cancer. It induces VEGF expression in vascular endothelial cells and functions as an autocrine factor for EC growth and angiogenesis. It is upregulated in atherosclerotic lesions and is elevated in the serum and cerebrospinal fluid following myocardial infarction. In cancer, IL-8 promotes epithelial-mesenchymal transition as well as tumor cell invasiveness and metastasis.

Item	Value
Long Name	Interleukin 8
Entrez Gene IDs	3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names	3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8, CXCL8SCYB8, Emoctakin, GCP-1TSG-1, GCP1, IL-8, IL8, K60, LAI, LECT, LUCT, LYNAP, MDNCF, MDNCFb-ENAP, MONAP, MONAPGCP1, NAF, NAP-1NAP1, NAP1, NCF, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, interleukin 8, member 8

Citations for Human IL-8/CXCL8 Quantikine HS ELISA Kit (31)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-8/CXCL8 Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Plasma

Z Chen et al. (2022-01-01), The involvement of copper, circular RNAs, and inflammatory cytokines in chronic respiratory disease Chemosphere, 303(0):135005.
PMID: 35605724
Species: Human
Sample Types: Plasma

RJ Pawlik et al. (2022-01-01), Circulating Pro-inflammatory Cytokines Do Not Explain Interindividual Variability in Visceral Sensitivity in Healthy Individuals Frontiers in Neuroscience, 16(0):876490.
PMID: 35860299
Species: Human
Sample Types: Serum

RM Critelli et al. (2022-01-01), Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma Oncology Letters, 24(5):395.
PMID: 36276500
Species: Human
Sample Types: Whole Blood

M Jamka et al. (2021-01-01), Endurance Training Depletes Antioxidant System but Does Not Affect Endothelial Functions in Women with Abdominal Obesity: A Randomized Trial with a Comparison to Endurance-Strength Training Journal of Clinical Medicine, 10(8).
PMID: 33921520
Species: Human
Sample Types: Cell Culture Supernates

Y Du et al. (2021-01-01), Nicotine regulates autophagy of human periodontal ligament cells through alpha7 nAchR that promotes secretion of inflammatory factors IL-1beta and IL-8 BMC Oral Health, 21(1):560.
PMID: 34732192
Species: Human
Sample Types: Cell Culture Supernates

M Yamaya et al. (2021-01-01), The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells Virus research, 304(0):198548.
PMID: 34425162
Species: Human
Sample Types: Serum

J Doll et al. (2020-01-01), LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy J Orthop, 17(0):208-214.
PMID: 31889743
Species: Human
Sample Types: cell culture supernatant

M Auclair et al. (2020-01-01), HIV antiretroviral drugs, dolutegravir, maraviroc and ritonavir-boosted atazanavir use different pathways to affect inflammation, senescence and insulin sensitivity in human coronary endothelial cells PLoS ONE, 15(1):e0226924.
PMID: 31971958
Species: Human
Sample Types: Serum

P Fatyga et al. (2020-01-01), The relationship between malnutrition risk and inflammatory biomarkers in outpatient geriatric population Eur Geriatr Med, 0(0).
PMID: 32297262
Species: Human
Sample Types: Plasma

T Reichel et al. (2020-01-01), Reliability and suitability of physiological exercise response and recovery markers Sci Rep, 10(1):11924.
PMID: 32681124
Species: Human
Sample Types: Plasma

E Schneck et al. (2020-01-01), Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study J Clin Med, 9(7).
PMID: 32629885
Species: Human
Sample Types: Serum

MH Hanindita et al. (2020-01-01), Comparison of two lipid emulsions on interleukin-1&beta, interleukin-8 and fatty acid composition in infants post gastrointestinal surgery:ï¿½a randomized trial F1000Research, 9(0):1168.
PMID: 33299555
Species: Human
Sample Types: Serum

AN Burska et al. (2020-01-01), Dynamics of Early Signalling Events during Fracture Healing and Potential Serum Biomarkers of Fracture Non-Union in Humans J Clin Med, 9(2).
PMID: 32054088
Species: Human
Sample Types: Serum

G Merckx et al. (2020-01-01), Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles Cells, 9(2).
PMID: 32012900
Species: Human
Sample Types: EBC, Exhaled Breath Condensate (EBC)

K Jaskiewicz et al. (2020-01-01), Exhaled Biomarkers in Idiopathic Pulmonary Fibrosis-A Six-Month Follow-Up Study in Patients Treated with Pirfenidone J Clin Med, 9(8).
PMID: 32764328
Species: Human
Sample Types: Cell Culture Supernates

ML Hartman et al. (2020-01-01), 17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-kappaB) Activity in Patient-Derived Melanoma Cell Lines Int J Mol Sci, 21(11).
PMID: 32466509
Species: Human
Sample Types: Serum

E Zuccolo et al. (2020-01-01), The microRNA-34a-Induced Senescence-Associated Secretory Phenotype (SASP) Favors Vascular Smooth Muscle Cells Calcification Int J Mol Sci, 21(12).
PMID: 32585876
Species: Human
Sample Types: Plasma

C Metzler-Ba et al. (2019-01-01), Sex-specific effects of central adiposity and inflammatory markers on limbic microstructure Neuroimage, 189(0):793-803.
PMID: 30735826
Species: Human
Sample Types: Plasma

E Kaasinen et al. (2019-01-01), Impact of constitutional TET2 haploinsufficiency on molecular and clinical phenotype in humans Nat Commun, 10(1):1252.
PMID: 30890702
Species: Human
Sample Types: CSF

E Krock et al. (2019-01-01), Interleukin-8 as a therapeutic target for chronic low back pain: Upregulation in human cerebrospinal fluid and pre-clinical validation with chronic reparixin in the SPARC-null mouse model EBioMedicine, 0(0).
PMID: 31047862
Species: Human
Sample Types: Serum

N Aziz et al. (2019-01-01), Biological variation of immunological blood biomarkers in healthy individuals and quality goals for biomarker tests BMC Immunol., 20(1):33.
PMID: 31521107
Species: Human
Sample Types: Tissue Homogenates

LA Sjaarda et al. (2018-01-01), Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation PLoS ONE, 13(5):e0197039.
PMID: 29750805
Species: Human
Sample Types: Serum

H Ugge et al. (2018-01-01), The influence of prostatic Cutibacterium acnes infection on serum levels of IL6 and CXCL8 in prostate cancer patients Infect. Agents Cancer, 13(0):34.
PMID: 30473726
Species: Human
Sample Types: Serum

E Kenne Sare et al. (2017-01-01), Mindfulness and its efficacy for psychological and biological responses in women with breast cancer Cancer Med, 0(0).
PMID: 28421677
Species: Human
Sample Types: Serum

R Critelli et al. (2017-01-01), Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study Cell Death Dis, 8(8):e3017.
PMID: 28837142
Species: Human
Sample Types: Whole Cells

I Zalesna et al. (2017-01-01), Exogenous growth factors bFGF, EGF and HGF do not influence viability and phenotype of V600EBRAF melanoma cells and their response to vemurafenib and trametinib in vitro PLoS ONE, 12(8):e0183498.
PMID: 28829835
Species: Human
Sample Types: Cell Culture Supernates

Pisetsky D et al. (2014-01-01), The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study. Cytokine, 69(1):110-5.
PMID: 25022969
Species: Human
Sample Types: Sputum

Singh R et al. (2014-01-01), Inflammatory thresholds and the species-specific effects of colonising bacteria in stable chronic obstructive pulmonary disease. Respir Res, 15(0):114.
PMID: 25218165
Species: Human
Sample Types: Plasma

Pinti M et al. (2014-01-01), Circulating mitochondrial DNA increases with age and is a familiar trait: Implications for "inflamm-aging" Eur J Immunol, 44(5):1552-62.
PMID: 24470107
Species: Human
Sample Types: Cell Culture Supernates

Proenca-Ferreira R et al. (2014-01-01), Endothelial activation by platelets from sickle cell anemia patients. PLoS ONE, 9(2):e89012.
PMID: 24551209
Species: Human
Sample Types: Plasma

Teichert T et al. (2013-01-01), Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study. PLoS ONE, 8(12):e83042.
PMID: 24340078

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