Targeted Protein Degradation

Target validation, the process of establishing that a potential molecular target is involved in a physiological function or disease process, is a key step in the development of new Degraders, as well as for drug discovery and biological research. Our TAG Degradation platform offers a differentiated method for target validation, using targeted protein degradation to reversibly knockdown a potential protein target and enable exploration of the downstream phenotype.

Targeted protein degradation can be achieved using small molecule Protein Degraders, such as PROTAC® molecules. Bio-Techne provides a toolbox of products and services to support your Degrader Design and Synthesis program and save you time and money, including our PROTAC® Panel Builder online tool; a large range of off-the-shelf Degrader Building Blocks; and tools for new E3 ligase ligand discovery.

Successful development of small molecule Protein Degraders for targeted protein degradation requires an array of assays to address a specific and differentiated set of questions aimed at characterizing aspects of Degrader activity. The assay workflow should explore: target engagement and ternary complex formation induced by the Degrader; ubiquitination of the target protein; as well as target degradation and its downstream consequences. Multiple orthogonal assays will maximize the chance of successful Degrader development. 

Bio-Techne through the R&D Systems^TM brand provides a wide range of products to help you explore and characterize ubiquitin biology. The rapidly expanding portfolio includes highly active E3 Ubiquitin Ligases, including SKP2, VHL, Cereblon (CRBN) and DCAF proteins, as well as assay kits and components, antibodies and Tandem Ubiquitin Binding Entities (TUBEs). We also provide Cullin-Rbx complexes (including neddylated cullins) that can be coupled to your purified substrate binding complexes to generate active ligases.

Bio-Techne offers a unique portfolio of custom services to support your PROTAC® discovery and development and target validation programs. Harness our expertise in custom chemistry to develop active degraders to knock down your target of choice or custom building block panels. We also offer custom E3 ligase development services enabling you to build your own assays to investigate ubiquitination, and custom knock-in cell lines expressing your target protein of interest as a fusion with a "TAG" protein for use within our TAG degradation platform.