Your Complete Solution for Targeted Protein Degradation
Bio-Techne offers a full range of products and services for Targeted Protein Degradation research and development, including Active Degraders (e.g. PROTAC degraders), TAG Degraders, Degrader Building Blocks, Ubiquitin-Proteasome System Proteins and Assays, Assays for Protein Degradation and Custom Degrader Services.
Browse Products and Services
Active Degraders
Active Degraders
PROTAC Degraders are a small molecule-based approach for the knockdown of target proteins within cells.
Degrader Building Blocks
Degrader Building Blocks
Bio-Techne provides chemical building blocks to support Degrader research and development.
TAG Degradation Platform
TAG Degradation Platform
Our aTAG and dTAG platforms enable selective protein knockdown for target validation.
Custom Degrader Services
Custom Degrader Services
We offer a range of custom services for Degrader research and development.
Ubiquitin-Proteasome System Products
Ubiquitin-Proteasome System Products
Explore our wide range of UPS-related proteins and assays.
Protein Degradation Assays
Protein Degradation Assays
Our automated Western blot assay systems are rapid, gel-free, blot-free, hands-free.
Meet Jess - Your Complete Protein Analysis Solution
Our Jess instrument automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps.
Targeted Protein Degradation Resources
Brochure: Targeted Protein Degradation Research
Brochure: Targeted Protein Degradation Research
Our TPD Research Product Guide highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation research including, Active Degraders, Degrader Building Blocks, Custom Degrader Services, Proteins and Assays for protein degradation.
Video: What is Targeted Protein Degradation?
Video: What is Targeted Protein Degradation?
Our short animation explains the fundamentals of Targeted Protein Degradation (TPD) using small molecule Degraders or PROTACs, that harness the ubiquitin-proteasome system to selectively remove proteins from cell.
Virtual Symposium: Targeted Protein Degradation
Virtual Symposium: Targeted Protein Degradation
This virtual symposium brought together leading scientists in the field of Targeted Protein Degradation to discuss their recent research and provide insights into PROTAC design, discovery and development, new research tools and expanding the E3 ligase toolbox.
Poster: Generating a Chemical Toolbox to Support PROTAC R&D
Poster: Generating a Chemical Toolbox to Support PROTAC R&D
Authored by in-house experts, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each component are discussed.
Research Paper: Developing Degraders: Principles and Perspectives
Research Paper: Developing Degraders: Principles and Perspectives
Tocris scientists have developed a set of guidelines relating to the physicochemical properties required in the design and synthesis of cell-permeable Degrader/ PROTAC molecules, which are reported in this paper in MedChemComm.
Publication Spotlight: Simple Western™ Assays & TPD Research
Publication Spotlight: Simple Western™ Assays & TPD Research
Learn how fully automated Simple Westerns are being used in research to measure Degrader-induced protein knockdown.
Background Information
How do Degrader molecules work?
Schematic showing the catalytic mode of action of Degrader molecules.
Targeted Protein Degradation is a technique that uses heterobifunctional small molecule Degraders, to bring about efficient and highly selective degradation of a target protein of interest. Targeted protein degradation enables investigation of the downstream consequences of protein knockdown and provides an easy-to-use method for target validation.
Bio-Techne provides Active Degrader molecules, including PROTAC Degraders and TAG Degraders (dTAG/aTAG). PROTAC molecules comprise a ligand that binds a target of interest joined by a linker to an E3 ligase ligand. A ternary complex is formed by the simultaneous binding of the Degrader to the target protein and the E3 ligase, which initiates ubiquitination of the target protein and its subsequent destruction by the proteasome. TAG Degraders work on a similar principle, but comprise an E3 ligase ligand linked to a compound that binds a fusion of mutant FKBP12F36V (for the dTAG system) or MTH1 (for the aTAG system) with a target protein of interest. These Degraders enable the knockdown of proteins for which there are no known ligands.
Bio-Techne supplies a range of other products and services to support Targeted Protein Degradation studies, including Degrader Building Blocks to enable you to create your own Degraders, as well as Ubiquitin-Proteasome System (UPS) Proteins and Assays, Assays for Protein Degradation, and Custom Degraders Services.
Why Use Degraders?
Small molecule Degraders offer several advantages over genetic manipulation for target protein knockdown:
- Degraders are cell-permeable, are suitable for use in vivo and in vitro, and can be applied directly to cells with no need for transfection or expression vectors.
- They are applicable to multiple cell lines with no requirement that cells are easily transfectable
- The duration of effect is adjustable and reversible on compound washout
- They have a catalytic mode of action repeatedly engaging and directing ubiquitination of target molecules, allowing use at sub-stoichiometric concentrations.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.