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Why use RNAscope ISH?

Most discovery tools and standard transcriptomic techniques such as qPCR and sequencing that are gold standard molecular methods for disease profiling, rely on dissociated cells which results in a loss of spatial information.

Inspired to solve these challenges, ACD scientists revolutionized the RNAscope™ assay, a novel multiplex nucleic acid in situ hybridization technology. ACD's unique patented probe design amplifies target-specific signals but not background noise, delivering clear and actionable results with high sensitivity and specificity.

Learn how the technology works

High Sensitivity & Specificity
High Sensitivity & Specificity
Single Cell Resolution
Single Cell Resolution
Patented ZZ Technology
Patented "ZZ" Technology
Any Genes, Any Species
Any Genes, Any Species
Over 2900 Peer Reviewed Publications
Over 2900 Peer Reviewed Publications
Multiomic Approach
Multiomic Approach

Customer Success Stories

Spatial Genomics Applications

Learn more about the RNAscope, BaseScope and miRNAscope assays that enable detection of diverse range of targets across broad applications such as Oncology, Neuroscience, Infectious disease, Developmental biology and more with multiplexing capabilities and visualization at a single cell resolution.

Enabling research, drug development and diagnostics

Quantitative Molecular Detection with Morphological Context and In Situ Hybridization (ISH)

Research-use-only products

Independent of your genes of interest, our easy RNA in situ hybridization assay uses common reagents and protocols; providing universal assay conditions.

RNAScope for Diagnostics

RNAscope™ Technology for Diagnostics

Robust, sensitive, and specific RNAscope RNA In Situ Hybridization Detection System available in chromogenic formats on most major automated stainers registered as an FDA Class 1 in the US and CE-IVD in the EU. Find out how the RNAscope technology, including our well-known high risk and low risk HPV probes, can support your diagnostic needs.

Pharma Assay Services PAS

Pharma Assay Services

Available for your immediate RNAscope, BaseScope, and miRNAscope outsourcing needs.

Vast experience in supporting thousands of pre-clinical and global clinical studies for global pharma and biotech partners, from discovery through assay development to clinical trials.

Download the Pharma Assay Service Brochure

New Offerings

Catalog and Made-to-Order Probes

Using proprietary ACD RNAscope™ Probe Design Pipeline, we provide double "Z" oligo probes designed to hybridize to your specific RNA target of interest.

Made to order probes
ACDBio probes for Sars-CoV2

Probes to Detect SARS-Cov2

Introducing new ACD Probe Sets which includes set of probes in pre-assigned channels for applications across Infectious Diseases and Neuroscience compatible with RNAscope Multiplex and HiPlex assays

Expand your multiplexing capabilities using

The RNAscope HiPlex & HiPlexUp Assay

Spatially map and confirm transcriptomic data identified using discovery tools using this high-throughput multiplexing assay. Now allowing simultaneously detection of up to 48 different RNA targets per slide mounted sample at a single cell resolution.

  • Spatially map a cell atlas
  • Visualize gene signatures of newly identified cell subtypes
  • Classify highly heterogeneous cell types
  • Confirm new therapeutic cell types
  • Characterize immune landscape
  • Identify new immunotherapy targets
  • Analyze or predict response to drug treatments
  • Confirm publicly available datasets such as TCGA and Tabula Muris
Deeper insights with RNAscope HiPlex & HiPlexUp Assay
Simultaneous Visualization of RNA and Protein Targets Using The New Co-detection Kit

Simultaneous Visualization of RNA and Protein Targets Using The New Co-detection Kit

ACD’s new Co-detection Assays will allow researchers to simultaneously examine cell-type specific gene expression and identify cellular sources of secreted proteins. The new workflow will allow inclusion of wider range of antibodies to be combined with RNA ISH enabling researchers to acquire more data and conserve precious samples.

  • Detect pathogens and host cell markers
  • Detect non-coding RNA in target cells
  • Identify cellular source of secreted proteins
  • Visualize cell margin and RNA targets
  • Correlate RNA-protein expression
  • Validate antibody specificity

Popular RNAscope ISH applications

Infectious Diseases

RNAscope technology provides new insights into the pathogenesis of infectious agents and aid in vaccine and drug development

Cancer

Understanding complex and heterogenous tumor microenvironment and its dynamics in cancer initiation and progression

Cell / Gene Therapy

RNAscope technology can be a valuable tool in safety and efficacy assessment of gene and cell-based therapies, including visualization of delivery vector and transgene in target tissue

Neuroscience

Interrogation of complex structural and functional heterogeneity of the nervous system by simultaneously detecting multiple targets at a single cell resolution

Single Cell

Validation and visualization of transcriptomic profiles of novel cell types identified by single cell analysis

Dual ISH/IHC

Simultaneous visualization of RNA and protein targets on the same slide using our co-detection workflow

For additional information on other application areas, visit www.acdbio.com

Related RNAscope Resources

Single-Cell Application Note

The RNAscope™ multiplex in situ hybridization technology enables the incorporation of spatial mapping and confirmation of gene signatures into single cell RNA sequencing workflows

RNAscope-GeoMx DSP White Paper

Molecularly Guided Highly Multiplexed Spatial Profiling with the RNAscope and GeoMx Assays

Dual ISH-IHC App note

Combining the RNAscope ISH technology with IHC to spatially resolve RNA and protein targets simultaneously