Stem Cell Isolation, Culture, Differentiation, and Characterization
Bio-Techne supports all stages of stem cell research, from isolation and culture to verification, reprogramming, differentiation, and characterization. We offer optimized and reproducible kits for cell isolation, multicolor flow cytometry for cell marker expression, immunoassays, stem cell differentiation, and single-cell analysis. Our premium reagents include cytokines and growth factors, extracellular matrix proteins, small molecules, antibodies, antibody panels, and specialized cell culture media.
Embryonic and Induced Pluripotent Stem Cells
Embryonic and Induced Pluripotent Stem Cells
We offer many tools for your research on ES and iPSC including irradiated mouse embryonic fibroblasts, mouse embryonic fibroblast conditioned media, antibody marker panels, and culture media supplements.
Organoids and 3D Cultures
Organoids and 3D Cultures
Generate functional tissue-like organoids and spheroids and perform toxicology testing. Our protocols and tissue-specific recipes cover optimized base media reagents, matrix components, growth factors, and small molecules.
Hematopoietic Stem Cells
Hematopoietic Stem Cells
Investigate HSC differentiation with our specialized media and supplements, lineage depletion antibodies panels, multi-color flow cytometry kits, and colony forming cell assay reagents.
Mesenchymal Stem Cells
Mesenchymal Stem Cells
Browse our kits and reagents for MSC research including optimized base media and supplements for osteogenic, adipogenic, and chondrogenic lineages plus lineage induction reagents and functional identification kits.
Neural Stem Cells
Neural Stem Cells
Our NSC catalog includes cortical stem cells, optimized culture supplements, differentiation kits for neurons and oligodendrocytes, functional identification kits, imaging dyes, and caged compounds.
Cancer Stem Cells
Cancer Stem Cells
For research into the functions of tissue-specific cancer stem cells, we offer CSC identification kits and antibodies, CSC population isolation kits, tumor spheroid assays and optimized media, and assays for cell migration and invasion.
Background Information
Stem cells are unspecialized cells that are capable of self-renewal through mitotic cell division, even after long periods of inactivity. Stem cell differentiation gives rise to the endoderm, ectoderm, and mesoderm germ layers which develop into the specialized cells of a tissue or organ.
Embryonic stem cells (ESCs) are found in the inner cell mass of blastocysts, adult (or somatic) stem cells maintain the populations of specific cell types, induced pluripotent stem cells (iPSCs) are produced by the reprogramming of somatic cells, and cancer stem cells (CSCs) reside in tissues and can give rise to local tumors. Hematopoietic stem cells (HSC) differentiate into the myeloid and lymphoid immune cell lineages. Multipotent mesenchymal stem cells (MSC) give rise to adipocytes, chondrocytes, osteocytes, and cardiomyocytes. Neural stem cells (NSC) can differentiate into neurons, astrocytes, and oligodendrocytes.
Owing to their regenerative potential, stem cells can be utilized to repair or replace old or damaged cells, and this property is valuable in regenerative medicine. Stem cells offer the potential for treating many diseases including cancer, neurodegeneration, musculoskeletal disorders, heart disease, and diabetes. 3-dimensional cultures and organoids based on differentiated stem cells reproduce some functions of native tissues and are valuable for toxicology studies.