Skip to main content

Human EMT 3-Color Immunocytochemistry Kit

Contains conjugated antibodies to human Snail-NL557, E-Cadherin-NL637, and Vimentin-NL493

Key Product Details

Upregulation of the Mesenchymal Markers, Snail and Vimentin, in EMT-induced Cells
Catalog #
Availability
Size / Price
Qty
Loading...
SC026

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, epithelial to mesenchymal transition (EMT) status in human cells can be analyzed via 3-Color ICC using the following protocol:

  • EMT is induced in cells of interest
  • Fluorochrome-conjugated primary antibodies are added to fixed cells
  • EMT markers are visualized using a fluorescence microscope
 

 

Reagents Provided

Reagents supplied in the Human EMT 3-Color Immunocytochemistry Kit (Catalog # SC026):

  • NL557-conjugated Goat Anti-Human Snail
  • NL637-conjugated Goat Anti-Human E-Cadherin
  • NL493-conjugated Goat Anti-Human Vimentin

This kit contains sufficient reagents to process up to 1 x 109 total cells.

 

Other Supplies Required

Reagents

  • Cells
  • Coverslips (sterilized)
  • Cell culture plate (24-well)

Materials

  • Human pluripotent stem cells
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C, 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • Fluorescence microscope

 

Procedure Overview

Plate human epithelial cells.

 

Culture under desired EMT-inducing conditions.

Plate human epithelial cells

Fix cells with 4% paraformaldehyde.

Fix cells with 4% paraformaldehyde

Block in blocking solution.

Block in blocking solution

Incubate with fluorochrome-conjugated primary antibodies.

Wash with wash buffer.

Incubate with fluorochrome-conjugated primary antibodies

Incubate with nuclear counterstain.

Incubate with nuclear counterstain

Visualize using a fluorescence microscope and appropriate filter sets

Citations (2)

Showing  1 - 2 of 2 citations Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human EMT 3-Color Immunocytochemistry Kit include:

There are no citations that match your criteria.

Showing  1 - 2 of 2 citations Showing All

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Complete Your Research

Complete Your Experiment