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Human EMT 3-Color Immunocytochemistry Kit

R&D Systems, Inc. a Bio-Techne Brand

Contains conjugated antibodies to human Snail-NL557, E-Cadherin-NL637, and Vimentin-NL493

Key Product Details

Upregulation of the Mesenchymal Markers, Snail and Vimentin, in EMT-induced Cells
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, epithelial to mesenchymal transition (EMT) status in human cells can be analyzed via 3-Color ICC using the following protocol:

  • EMT is induced in cells of interest
  • Fluorochrome-conjugated primary antibodies are added to fixed cells
  • EMT markers are visualized using a fluorescence microscope


Reagents Provided

Reagents supplied in the Human EMT 3-Color Immunocytochemistry Kit (Catalog # SC026):

  • NL557-conjugated Goat Anti-Human Snail
  • NL637-conjugated Goat Anti-Human E-Cadherin
  • NL493-conjugated Goat Anti-Human Vimentin

This kit contains sufficient reagents to process up to 1 x 109 total cells.


Other Supplies Required


  • Cells
  • Coverslips (sterilized)
  • Cell culture plate (24-well)


  • Human pluripotent stem cells
  • Pipettes and pipette tips
  • Serological pipettes


  • 37 °C, 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • Fluorescence microscope


Procedure Overview

Plate human epithelial cells.


Culture under desired EMT-inducing conditions.

Plate human epithelial cells

Fix cells with 4% paraformaldehyde.

Fix cells with 4% paraformaldehyde

Block in blocking solution.

Block in blocking solution

Incubate with fluorochrome-conjugated primary antibodies.

Wash with wash buffer.

Incubate with fluorochrome-conjugated primary antibodies

Incubate with nuclear counterstain.

Incubate with nuclear counterstain

Visualize using a fluorescence microscope and appropriate filter sets

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Product Documents for Human EMT 3-Color Immunocytochemistry Kit

Certificate of Analysis

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