Human EMT 3-Color Immunocytochemistry Kit
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Refer to the product datasheet for complete product details.
Briefly, epithelial to mesenchymal transition (EMT) status in human cells can be analyzed via 3-Color ICC using the following protocol:
- EMT is induced in cells of interest
- Fluorochrome-conjugated primary antibodies are added to fixed cells
- EMT markers are visualized using a fluorescence microscope
Reagents supplied in the Human EMT 3-Color Immunocytochemistry Kit (Catalog # SC026):
- NL557-conjugated Goat Anti-Human Snail
- NL637-conjugated Goat Anti-Human E-Cadherin
- NL493-conjugated Goat Anti-Human Vimentin
This kit contains sufficient reagents to process up to 1 x 109 total cells.
- Coverslips (sterilized)
- Cell culture plate (24-well)
- Human pluripotent stem cells
- Pipettes and pipette tips
- Serological pipettes
- 37 °C, 5% CO2 incubator
- Inverted microscope
- Fluorescence microscope
Plate human epithelial cells.
Culture under desired EMT-inducing conditions.
Fix cells with 4% paraformaldehyde.
Block in blocking solution.
Incubate with fluorochrome-conjugated primary antibodies.
Wash with wash buffer.
Incubate with nuclear counterstain.
Visualize using a fluorescence microscope and appropriate filter sets
Citations for Human EMT 3-Color Immunocytochemistry Kit (2)
Citations are publications that use Bio-Techne products. Selected citations for Human EMT 3-Color Immunocytochemistry Kit include:
Sample Types: Whole Cells
YL Liu et al. (2019), Assessing metastatic potential of breast cancer cells based on EGFR dynamics Sci Rep, 9(1):3395.
Sample Types: RWPE-1
S Sterbova et al. (2018), Oncostatin M induces tumorigenic properties in non-transformed human prostate epithelial cells, in part through activation of signal transducer and activator of transcription 3 (STAT3) Biochem. Biophys. Res. Commun., 0(0).
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