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Featured Bio-Techne Antibody Products
Antibodies for Immune Cell Therapy
Antibodies for Immune Cell Therapy
Find highly specific monoclonal and recombinant monoclonal antibodies for activation, expansion, and characterization of T cell and NK cell therapies.
Antibodies for IVD Assay Development
Antibodies for IVD Assay Development
Bio-Techne offers highly validated and specific matched antibody pairs for the development of in vitro diagnostic (IVD) assays.
Antibodies for GPCR Therapeutic Development
Antibodies for GPCR Therapeutic Development
Bio-Techne’s R&D System brand has been a leader in the development of GPCR antibodies. We offer the largest selection of highly validated GPCR antibodies in the industry.
Antibodies for Coronavirus Research
Antibodies for Coronavirus Research
Bio-Techne offers a large selection of antibodies for SARS-CoV-2 and COVID-19 research including for viral detection, investigating infection mechanisms, and characterizing immune responses.
Full Bio-Techne Antibody Portfolio
Primary Antibodies
Primary Antibodies
Bio-Techne® offers a large portfolio of primary antibodies validated for over 15 applications and in 25+ species. Multiple size options available including sample and bulk sizes.
Secondary Antibodies
Secondary Antibodies
Bio-Techne offers species-specific secondary antibodies for Western blot, flow cytometry, immunocytochemistry, and immunohisto- chemistry. Secondary antibodies are available with a variety of conjugated labels.
Recombinant Monoclonal Antibodies
Recombinant Monoclonal Antibodies
Bio-Techne offers a large selection of recombinant monoclonal antibodies that display excellent sensitivity and consistency in order to reduce variability in your experiments.
Knockout Validated Antibodies
Knockout Validated Antibodies
Bio-Techne offers an extensive selection of antibodies whose specificity has been confirmed with cells and lysates from an engineered knockout cell line.
Conjugated Primary Antibodies
Conjugated Primary Antibodies
Customize your experiments with Bio-Techne’s conjugated primary antibodies. Choose from our large selection of fluorescent and enzymatic conjugates to easily analyze multiple targets.
Epitope Tag Antibodies
Epitope Tag Antibodies
Bio-Techne offers antibodies against the most commonly used epitope tags for use in many different immunoassays including Western blot and immunoprecipitation.
Isotype Control Antibodies
Isotype Control Antibodies
Bio-Techne offers a wide range of isotype control antibodies, both conjugated and unconjugated, for a multitude of species and antibody isoforms for use as a negative control in immunoassays.
Biosimilar Antibodies
Biosimilar Antibodies
Bio-Techne offers biosimilar antibodies to facilitate studies based on a variety of monoclonal therapeutics or to use as controls with our anti-idiotype antibodies. They are recombinantly expressed using the same sequence of its respective approved biologic.
Anti-Idiotype Antibodies
Anti-Idiotype Antibodies
Bio-Techne offers multiple types of anti-idiotype antibodies, both blocking and non-blocking, for pharmacokinetic and immunogenicity studies.
View R&D Antibody Portfolio at A Glance
Choose from a high-performance portfolio so you can focus on the science.
Explore Traditional IHC, Multiplexing, and RNA-Protein Co-detection
Our NEW handbook helps you understand, perform, optimize, and troubleshoot your IHC assays.
Custom Antibody Services
If you can’t find the antibody that you need on our website, contact us. Leverage the experience and skill of our antibody development scientists for your custom antibody project.
5 Pillar Validation - Antibodies You Can Trust
Bio-Techne has always been at the forefront of antibody validation testing. We carefully test every antibody we produce, following our established validation procedures as well as the 5 Pillars of Antibody Validation, so that you can be confident in the results generated with our antibodies.
Simple Western™ - Move Beyond Traditional Western Blots
Simple Western™ systems are a gel-free, blot-free, hands-free Western blotting platform. Simple Western precisely controls the separation, washing, antibody incubations, and detection steps of traditional Western blots inside of a nanocapillary. Get highly reproducible and fully quantitative data with only 3 µL of sample in as little as 3 hours using conventional Western blot antibodies.
Get a Free Detection or Separation Module! Submit Simple Western Antibody Validation Data
Download Bio-Techne's Flow Cytometry Handbook
Download Bio-Techne’s Flow Cytometry Handbook to learn about the important aspects of flow cytometry. This handbook provides an overview of the flow cytometry technique and explains how a flow cytometer works and the basic principles that underlie cell sorting, complete with numerous data examples. It also gives step-by-step protocols and troubleshooting tips, making it easier for you to begin your flow cytometry experiments.
Related Antibodies Resources
Validating Antibodies to Support AstraZeneca Drug Discovery
Validating Antibodies to Support AstraZeneca Drug Discovery
This webinar outlines our strategies for ensuring appropriate antibody selection and validation. We are joined by a scientist from the AstraZeneca Affinity Reagent Group who describes how we apply our antibody expertise to deliver fit-for-purpose antibodies for their drug discovery pipeline.
Relax and Go with the Flow: Demystifying Multi-Parameter Flow Cytometry
Relax and Go with the Flow: Demystifying Multi-Parameter Flow Cytometry
This is the first part of a two-part webinar series from the Flow Development Scientists at Bio-Techne. In this webinar you will learn about the fundamentals of flow cytometry including spectral overlap and compensation, how to choose the best fluorochrome, data analysis basics.
Turning Flow Cytometry Upside Down and Inside Out
Turning Flow Cytometry Upside Down and Inside Out
In this second part of a two-part webinar series, Flow Development Scientists from Bio-Techne discuss how to apply flow cytometry basics to various applications such as cell proliferation, dead cell exclusion, and the staining of intracellular proteins.
Development of Validated Monoclonal Antibodies
Development of Validated Monoclonal Antibodies
This white paper uses data examples to illustrate the stringent steps we take when validating our monoclonal antibodies. In reading this article, you will learn about our validation process including antigen design, feasibility testing, specificity and sensitivity testing, and release decisions and quality control.
IHC/ICC Protocol Guide
IHC/ICC Protocol Guide
This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful IHC or ICC Contributors include scientists from our IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications and our expert technical service team who have guided researchers through a range of IHC/ICC-related issues.
Custom Antibody Services
Custom Antibody Services
Leverage our three decades of antibody development experience for your custom antibody needs. Out antibody services include immunization and purification, recombinant conversion, and engineering services.
Background Information
Antibodies are large glycoproteins produced by the immune system to help defend the host against foreign invaders. They are Y-shaped molecules that are composed of two identical heavy and light polypeptide chains. One heavy and light chain associate via their N-terminals, forming a domain that will bind to its antigen. An antigen is a substance that initiates an immune response. It is typically a protein, but may also be a carbohydrate, small molecule or nucleotide. Each antibody will only recognize and bind to one specific epitope (i.e. site) on an antigen. This specific binding allows antibodies to be powerful research tools for the detection of a specific protein in an assay.
Monoclonal antibodies are a homogenous population of antibodies that are produced by a single clone of plasma B cells. These antibodies bind to the same epitope on an antigen. Polyclonal antibodies are a heterogeneous mixture of antibodies that is produced by different clones of plasma B cells. The antibodies recognize the same antigen but bind to different epitopes on the antigen.
Recombinant antibodies are monoclonal antibodies that are produced by molecular cloning techniques instead of traditional hybridoma-based technologies. In other words, hybridomas and animals are not needed to produce recombinant antibodies. Instead, genes for specific light and heavy antibody chains are inserted into expression vectors, which are subsequently introduced into expression host cells. The host cells produce the antibodies, which can then be collected. Advantages of recombinant monoclonal antibodies includes improved lot-to-lot consistency and reproducibility, ability to scale up to gram quantities, and opportunity for high-throughput, animal-free production.
Bio-Techne has adopted the recommendations outlined by the International Working Group for Antibody Validation, which was published in 2016 in Nature Methods (Uhlen, M. et al. (2016) Nat. Methods 13:823), for antibody validation. The methods we utilize include genetic validation via gene editing and knockout cell lines, orthogonal validation using RNAscope®in situ hybridization technology, independent antibody validation using several antibodies for the same protein, validation using a tagged protein standard, and validation across multiple cell lines, including positive and negative expressing cells, and multiple species. Visit our Antibodies Built for Reproducibility page on the R&D Systems site to learn more.
Bio-Techne takes rigorous steps toward producing antibodies that display the same high specificity from lot-to-lot. We develop and manufacture over 90% of our products in house, giving us control over product development and testing. The immunogens we use to produce our antibodies are designed to ensure correct epitope presentation using our high-quality proteins, transfected cells, or carefully selected peptide sequences. The new antibody lots are then screened beside previous commercial lots to ensure the new lots exhibit the same sensitivity and specificity. Visit our Antibodies Built for Reproducibility page on the R&D Systems site to learn more.
Gene Engineering Services
Realize the potential of gene engineering by partnering with our experts. Engineer any cell type including T cells and iPSC with our non-viral TcBuster™ gene delivery system.
For cell and gene therapy manufacturing, we offer GMP services that include process development for cell engineering, quality control, custom cell vialing, and regulatory support.
