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Cultrex Basement Membrane Extracts

• Different formats of organoid-qualified Cultrex Basement Membrane Extracts are available to promote robust cell growth, viability, and culture consistency

• Our latest, most advanced Cultrex UltiMatrix RGF Basement Membrane Extract has been optimized to improve the performance and consistency of spheroid, organoid, and stem cell cultures

• Reduced growth factor formulations provide more defined culture systems

• Quality controlled for lot-to-lot performance consistency

Recombinant Proteins for Organoid Culture

• We offer a complete catalog of R&D SystemsTM proteins commonly used for organoid culture, including even the most difficult to express and purify proteins such as R-Spondin-1, Noggin, and Wnt-3a

• All of our recombinant proteins are rigorously tested for bioactivity and lot-to-lot consistency 

• Each new production lot of protein is tested to ensure that it meets our expectations for endotoxin specification, which is an industry-leading <0.1 EU/µg

• R&D Systems proteins are highly cited in organoid-related scientific publications and frequently display greater activity than leading competitors’ products

• Our recombinant proteins can be ordered in bulk at a discounted price and custom protein services are also available

 

Small Molecules for Organoids

• A large selection of TocrisTM small molecules that are commonly used as 3-D growth matrix components and additives for long-term organoid growth are available

• Our catalog includes the ALK kinase inhibitor, A 83-01, the highly selective GSK-3 inhibitor CHIR 99021, the potent ROCK inhibitor, Y-27632 dihydrochloride, the p38 MAPK inhibitor, SB 202190, and many more

Media and Media Supplements

• We offer a variety of different formats of base media for organoid cultures, including media with or without glucose, L-glutamine, HEPES, NEAA, nucleosides, phenol red, or sodium pyruvate content

• We develop and manufacture serum-free media supplements to support organoid cultures

• Our catalog includes cryopreservation media, which is optimized to increase cell recovery and viability

Antibodies for Detecting Lineage-Specific Markers

• Our catalog includes over 780,000 R&D Systems™ and Novus Biologicals™ antibodies for more than 25 species

• We offer both unlabeled and labeled primary antibodies with 20 different potential fluorescent conjugates

• Our primary antibodies are validated for one or more of up to 15 applications

• We offer both conjugated and unconjugated secondary antibodies and a range of isotype control antibodies

• Our antibodies come in a variety of size options, including bulk and sample sizes to ensure that you can find what you need for your research

 

RNAscope In Situ Hybridization Assays

• Utilize this technology to spatially map and quantify specific target mRNAs in intact cells and tissues

• The assay can be combined with immunohistochemistry or immunofluorescence on the same slide to investigate mRNA and protein co-expression

• Up to 12 RNA targets can be detected simultaneously using the RNAscope HiPlex assay

• Catalog probes are available for over 23,000 targets in 140+ species and can also be made-to-order

• RNAscope ISH Assays are cited in over 2,600 publications across multiple different research areas

Video of Human Intestinal Organoid Grown in Cultrex UltiMatrix RGF BME

MUC2 (magenta) expression in human intestinal organoid grown using Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05). Images courtesy of LifeCanvas Technologies.

Human Lung Organoids Grown in Cultrex UltiMatrix RGF Basement Membrane Extract. Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured in media for 20-60 days. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. A) Lung organoids were stained with anti-Cytokeratin 5 (KRT5) (green; Novus Biologicals, Catalog # NB110-56916) and with anti-p63/TP73L (red; R&D Systems, Catalog # AF1916) to visualize basal cells. B) Lung organoids were stained with anti-Podoplanin (PDPN) (green; Novus Biologicals, Catalog # NB600-1015) to visualize alveolar type I cells. C, D) Lung organoids were stained with anti-Muc5ac (green; Novus Biologicals, Catalog # NBP2-15196) to visualize Goblet cells and with anti-Sox2 (red; R&D Systems, Catalog # MAB2018). All samples were counterstained with the nuclear stain DAPI (blue; Tocris, catalog # 5748).

Human lung organoids grown in Cultrex UltiMatrix RGF BME and stained for Cytokeratin 5, p63, Podoplanin, Muc5a, and SOX2.

Human Descending Colon Organoids. Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and cultured in growth medium for 30 days prior to imaging. A, B) Descending colon organoids were stained with Chromogranin A (green; R&D Systems, Catalog # MAB90981) to visualize intestinal enteroendocrine cells, and counterstained for E-Cadherin (red; R&D Systems, Catalog # AF748) and DAPI (blue; R&D Systems, Catalog # 5748).

Human descending colon organoids stained with Chromogranin A and counterstained with E-Cadherin and DAPI.

Cultrex UltiMatrix Supports the Culture of Human Induced Pluripotent Stem Cells (hiPSCs). iBJ6 hiPSCs were cultured on plates coated with Cultrex UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) diluted 1:100 (100 ug/mL) using DMEM. hiPSC colonies were immunofluorescently stained using F-show the typical F-actin stress fibers (green) that define hiPSC colony edges as well as nuclear expression of the pluripotency marker OCT3 (red).

Immunofluorescent staining of F-actin and OCT3 in human iPSCs grown in Cultrex UltiMatrix RGF Basement Membrane Extract.

Additional Organoid Resources

Organoid Culture Protocols

To assist researchers, R&D Systems cell culture scientists are working every day to optimize the culture conditions for growing different types of organoids. As we see success, the recipes and protocols are being published on the Organoid and 3-D Cell Culture Protocols page of our website.

Virtual Organoid Symposium

Watch Bio-Techne’s Virtual Organoid Symposium from May 2020 to learn more about how leading scientists are utilizing organoids in their research. Presenters include Mr. Jens Puschof and Dr. Talya Dayton from Dr. Hans Clevers lab at the Hubrecht Institute in the Netherlands, and Dr. Robert Barrett from Cedars Sinai Medical Center in Los Angeles, CA.

Evolution of Cell Culture Model Systems eBook

Download this eBook to learn about the history, current state, and future use of cell-based 2-D and 3-D model systems for studying disease conditions and drug screening. Key topics addressed are the use of cell lines and primary cells for toxicology, pluripotent stem cells models, 2-D vs. 3-D cell culture, the benefits and challenges of organoid models, and toxicology assays.

UltiMatrix Scientific Poster

Explore the data presented in this scientific poster to learn about the features of Cultrex UltiMatrix RGF Basement Membrane Extract that make it an ideal matrix for organoid cultures. This piece includes competitor comparison data showing that UltiMatrix Basement Membrane Extract has more consistent protein concentrations and gelling rates across lots than the leading commercial basement membrane extract.

Cell Culture Products

Browse our collection of products for cell culture. We offer a comprehensive range of reagents to promote robust cell growth, including media and supplements, FBS, basement membrane extracts, recombinant proteins, and custom cell culture services.

Stem Cells Research Area

Minimize variability in your stem cell experiments and ensure high performance by using our stem cell culture reagents. From isolation and culture to verification, reprogramming, differentiation, and characterization, we have products that you need for your stem cell work.