DAPI

Name: DAPI
Brand: Tocris Bioscience
SKU: 5748
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 5748

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Key Product Details

Description:	Fluorescent DNA dye; DAPI can be used for cell staining and assessing apoptosis; suitable for flow cytometry; DAPI mw and DAPI ex/em, provided
Alternative Names:	DAPI Dye
Chemical Name:	2-[4-(Aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide hydrochloride
Purity:	≥95% (HPLC)
Molecular Weight:	350.25

View Full Technical Details for DAPI

Biological Activity for DAPI

DAPI is a fluorescent DNA stain / dye. DAPI stain binds to AT-rich regions of DNA and fluorescence increases by approximately 20-fold when bound to double-stranded DNA. DAPI stain can be used in flow cytometry, DNA visualization and quantification in histochemistry/biochemistry, to assess apoptosis, and to visualize mouse embryos or fetal organs. DAPI stain can be used in fixed cells and is cell-permeable at high concentrations providing effective cell staining. It is reported to be more photostable than Hoechst 33342 (Cat. No. 5117). Em/Ex λ = 350/470 nm respectively.

Optical Data for DAPI

Emission Color	Blue
λ_abs	350 nm
λ_em	470 nm
Closest Laser line	405 nm
Application	Flow cytometry, ISH, Confocal Microscopy, Immunofluorescence (IHC, ICC)

Scientific Data Examples for DAPI

	DAPI staining. HeLa cells were fixed for 10 min with 4% paraformaldehyde and permeabilized. DAPI was added at 0.1 μg/mL and stained cells were imaged with an LSM880 confocal microscope (Obj. 63X/1.4 oil). Scale bar = 10 μm.
	DAPI counterstaining alongside MitoBrilliant™ Live 646. HeLa cells incubated with MitoBrilliant™ Live 646 (Catalog # 7417), 100 nM for 40 min. Cells were counterstained with DAPI. Image taken using an LSM880 Confocal using a 63X objective. Scale bar = 10 μm.
	RNAScope™ Assay with DAPI Counterstain. 3-plex RNAScope™ Multiplex Fluorescent v2 Assay on HeLa cells with TSA Vivid™ dyes 520, 570 and 650 (Catalog # 7523, 7526 and 7527, Tocris). Nuclei are counter-stained with DAPI stain (blue). All dyes were used at 1:1500 dilution. Markers shown are Polr2a (green), PPIB (red) and UBC (white).

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for DAPI

M. Wt	350.25
Formula	C₁₆H₁₅N₅.2HCl
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	28718-90-3
PubChem ID	160166
InChi Key	FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles	N=C(N)C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DAPI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	35.02	100
	water	3.5	10 with gentle warming

Preparing Stock Solutions for DAPI

The following data is based on the product molecular weight 350.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.86 mL	14.28 mL	28.55 mL
5 mM	0.57 mL	2.86 mL	5.71 mL
10 mM	0.29 mL	1.43 mL	2.86 mL
50 mM	0.06 mL	0.29 mL	0.57 mL

Molarity Calculator

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Reconstitution Calculator

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References for DAPI

References are publications that support the biological activity of the product.

Sandell DAPI staining of whole-mount mouse embryos or fetal organs. Cold Spring Harb.Protoc. 2018 PMID: 30275072
Chazotte Labeling nuclear DNA using DAPI. Cold Spring Harb.Protoc. 2011 PMID: 21205856
Kapuscinski DAPI: a DNA-specific fluorescent probe. Biotech.Histochem. 1995 PMID: 858020
Mildner Interaction of 4'-6-diamidino-2-phenylindole to nucleic acids, and its implication to their template activity in RNA-polymerase reaction of E. coli bacteria and of Friend-virus infected mouse spleen. Cancer Lett. 1978 PMID: 348301
Russell A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 1975 PMID: 46112

Citations for DAPI

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Customer Reviews for DAPI (2)

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DAPI staining of PC3 cells to detect apoptosis

Name: Anonymous

Species: Human

DAPI staining of PC3 cells treated with DR5 monoclonal antibodies to detect apoptosis and observing it with Floid Fluorescence Microscopy System.
Excellent counter-stain...

Name: Purnima Gupta

Species: Mouse

Assay Type: In Vivo

Cell Line/Tissue: Brain

Used as counter stain in IHC

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