Hoechst 33342

Name: Hoechst 33342
Brand: Tocris Bioscience
SKU: 5117
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 5117

Citations (3)

(1)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Fluorescent dye for staining DNA
Chemical Name:	2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	561.93

View Full Technical Details for Hoechst 33342

Biological Activity for Hoechst 33342

Hoechst 33342 stains and binds minor groove of AT-rich regions. Used to quantify DNA in viable cells. Cell permeable fluorescent DNA dye.

Optical Data for Hoechst 33342

λ_abs	350 nm
λ_em	461 nm
Application	Flow Cytometry

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Hoechst 33342

M. Wt	561.93
Formula	C₂₇H₂₈N₆O.3HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	875756-97-1
PubChem ID	16760503
InChi Key	JABNPSKWVNCGMX-UHFFFAOYSA-N
Smiles	CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hoechst 33342

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	28.1	50
	DMSO	28.1	50

Preparing Stock Solutions for Hoechst 33342

The following data is based on the product molecular weight 561.93.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	3.56 mL	17.80 mL	35.59 mL
2.5 mM	0.71 mL	3.56 mL	7.12 mL
5 mM	0.36 mL	1.78 mL	3.56 mL
25 mM	0.07 mL	0.36 mL	0.71 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for Hoechst 33342

References are publications that support the biological activity of the product.

Portugal and Waring Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 1988 PMID: 2449244
Loken Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1980 PMID: 7028425

Citations for Hoechst 33342 (3)

Showing 1 - 3 of 3 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Hoechst 33342 include:

Yang et al. (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy. :1
PMID: 30871407
Durymanov et al. (2019) Subcutaneous Inoculation of 3D Pancreatic Cancer Spheroids Results in Development of Reproducible Stroma-Rich Tumors. Transl Oncol. 12:180
PMID: 30554606
Tabdanov et al. (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep. 25:328
PMID: 30304674

There are no citations that match your criteria.

Showing 1 - 3 of 3 citations Showing All

Customer Reviews for Hoechst 33342 (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Nuclear labeling

Name: Anonymous

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: HEK 293

Immunostaining of HEK 293 cells

Works well at 1:2500 dilution incubated for 20 minutes at room temperature just before mounting the coverslips with the mounting media

There are no reviews that match your criteria.