Hoechst 33342
Tocris Bioscience, part of Bio-Techne | Catalog # 5117
Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy
Key Product Details
Description
Blue-fluorescent dye for DNA staining. Cell permeable. Used for: nuclear counterstain, apoptosis analysis, fixed and live-cell staining. Application: flow cytometry, confocal microscopy
Product Description
Key information: Hoechst 33342 is a blue-fluorescent dye for DNA staining. Cell permeable. Suitable for fixed and live-cell staining. Commonly used as a counterstain in fluorescence microscopy.Used for: nuclear counterstain, DNA visualization, cell cycle studies, apoptosis analysis.
Application: flow cytometry, confocal microscopy.
Properties and Photophysical Data: Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Excitation and emission maxima (λ) are 350 nm and 461 nm, respectively.
Scientific Data Images for Hoechst 33342
Hoechst 33342 | CAS No. 875756-97-1
Fluorescent dye for staining DNAOptical Data
| Emission Color | Blue |
| λabs | 350 nm |
| λem | 461 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Confocal Microscopy |
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Product Specifications
Molecular Weight
561.93
Formula
C27H28N6O.3HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride
CAS Number
875756-97-1
PubChem ID
16760503
InChI Key
JABNPSKWVNCGMX-UHFFFAOYSA-N
SMILES
CN(CC2)CCN2C1=CC(N=C(C4=CC(NC(C6=CC=C(OCC)C=C6)=N5)=C5C=C4)N3)=C3C=C1.Cl.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 28.1 | 50 | |
| DMSO | 28.1 | 50 |
Preparing Stock Solutions
for Hoechst 33342
The following data is based on the product molecular weight 561.93
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.56 mL | 17.80 mL | 35.59 mL |
| 2.5 mM | 0.71 mL | 3.56 mL | 7.12 mL |
| 5 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 25 mM | 0.07 mL | 0.36 mL | 0.71 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Loken Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry 1980 PMID: 7028425
- Portugal and Waring Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochim.Biophys.Acta. 1988 PMID: 2449244
Product Specific Notices for Hoechst 33342
For research use only
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