Recombinant Proteins

R&D Systems: The Most Trusted Source for Recombinant Proteins

Bio-Techne offers more than 5,000 R&D Systems® recombinant proteins for a variety of different research areas including immunology, cancer, development, neuroscience, and stem cells. With over 30 years of experience, R&D Systems’ rigorous in-house testing and quality control specifications ensure that our recombinant proteins will provide industry-leading bioactivity and purity, lot-to-lot consistency, and low endotoxin levels. As more than 95% of the proteins in our catalog are manufactured in-house, we maintain complete control over the quality of our products and the satisfaction of our customers, which is our number one priority.

To guarantee that each protein meets our customers’ high standards, we fully assess and report the specifications of each protein on our datasheets, including the activity, endotoxin level, and purity. The bioactivities of our proteins are assessed using a variety of different assays depending on the protein being tested. These include cell proliferation, chemotaxis, or cytokine secretion assays, enzymatic assays, functional ELISAs, and surface plasmon resonance (SPR). N-terminal sequence analysis is also performed for each recombinant protein to confirm that the protein produced matches the expected sequence, and the bioactivity and physical state of our proteins are monitored over long periods of time. Freeze/thaw cycles are carried out to ensure that the buffer provides for long-term stability. Additionally, we evaluate lot-to-lot consistency so that researchers don’t have to worry about whether or not their experiments will be reproducible over time. Multiple lots with matching specifications are created for all new proteins and each new lot is tested side-by-side with previous lots to ensure that the new lots display the same level of bioactivity and purity as the previous lots. Each new lot is also tested to make sure that it meets our expectations for endotoxin specifications, which is an industry-leading  <0.1 EU/µg. Finally, all of our recombinant proteins are specially formulated for the best stability and are lyophilized from a sterile filtered solution. If our documentation fails to address any questions that you may have about our products, our protein scientists and highly trained technical service team at R&D Systems are always available to assist you.

Our catalog includes recombinant proteins for multiple different species, that are either untagged or have a biotin, Fc, or His tag.  We also offer an expanding line of Avi-tag biotinylated proteins. Our standard recombinant proteins are available without or with a carrier protein, which is typically bovine serum albumin (BSA) at a concentration of 50 µg BSA per 1 µg of protein. The presence of the carrier protein gives the lyophilized protein greater stability, maximizes recovery upon reconstitution, and allows easy handling and storage. In addition to the wide selection of proteins that are manufactured under standard conditions, our portfolio also includes R&D Systems Animal-Free™ and GMP-grade recombinant proteins, ProDots® proteins, and custom protein development services.