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Ubiquitin-related enzymes

Ubiquitin-related Enzymes

• The highest purity E1 activating enzymes for Ubiquitin, SUMO, NEDD8, ISG15, and other Ubiquitin-like proteins

• The widest selection of E2 conjugating enzymes, including E2s for HECT-, RBR- and RING-type E3s, monoubiquitylating E2s, K11-, K48-, and K63-specific chain building E2s, and the SUMO and Neddylation E2s

• The largest collection of highly active E3 ligases, including HECT-, RBR-, SCF-, and other RING classes

• The most active, full-length recombinant deubiquitinase enzymes on the market

• Tandem ubiquitin-binding entities (TUBEs) for the selective capture of polyubiquitinated proteins

• Proteasome complexes including the 19S, 20S, and 26S proteasomes, the 20S immunoproteasome and the PA28 a, b, or g regulatory complexes

• Custom services for polyubiquitin characterization, DUB profiling, and protein purification are available to meet every researcher’s need

 

Glycobiology-related Enzymes

Glycobiology-related Enzymes

• A large selection of fucosyltransferases with clickable substrate GDP-Azido-Fucose for investigating core-, terminal-, and protein O-fucosylation modifications

• Sialyltransferases with clickable substrate CMP-Azido-Sialic Acid and CMP-C9-Biotin-Sialic Acid for exploring alpha2-3, alpha2-6, and alpha2-8 sialylation of different glycoproteins or glycolipids

• A wide selection of highly active N-acetylglucosaminyltransferases with clickable substrates UDP-Azido-GlcNAc and N-acetylgalactosaminyltransferases with clickable substrate UDP-Azido-GalNAc

• Highly active heparinases, heparanases, chondroitinases, and hyaluronidases for studying various glycosaminoglycans

• The largest collection of human lysosomal glycosidases that can be used as enzymatic tools as well as drug targets

 

Immunotherapy Enzymes

Immunotherapy-related Enzymes

• A large selection of enzymes involved in amino acid metabolism that are potential immunotherapeutic targets, including IDO, TDO2, ARG1, and ARG2

• Highly active, angiogenesis-promoting matrix metalloproteinases, including MMP-2, MMP-9, and MT1-MMP/MMP-14, and broad spectrum and selective MMP inhibitors

• The ectoenzymes, CD39 and CD73, which generate extracellular adenosine and contribute to immunosuppression in the tumor microenvironment

• A wide range of metabolic enzymes and inhibitors for investigating metabolic alterations in tumor cells

• A complete collection of kinases and kinase inhibitors for exploring cancer cell signaling pathways

Kinases and Phosphotases

Kinases and Phosphatases

• A large selection of active intracellular kinases, including ERK1, ERK2, MEK2, p38, MSK1, and ASK1, for studying protein phosphorylation

• The largest selection of potent and selective kinase inhibitors

• A substantial collection of phosphatases and phosphatase inhibitors for investigating the effects of protein dephosphorylation

 

Bioprocessing Enzymes

Bioprocessing Enzymes

• Nucleic acid processing enzymes, including NucA and Cas9

• A wide selection of highly active heparinases, heparanases, chondroitinases, and hyaluronidases for studying various glycosaminoglycans

• Economical, highly active glycosidases including PNGase F and O-Glycosidase for understanding the structures and functions of glycoproteins

• Aspartic proteases, cysteine proteases, serine proteases, and metalloproteases, along with protease inhibitors and substrates for understanding the functions and substrate specificities of different proteases

 

Bulk Enzymes

Bulk Enzymes

• The expertise and ability to scale up the production of almost any enzyme

• Specialized formulations available

• Economical pricing

Enzyme-Related Resources

Enzyme Assay Protocols

Explore our collection of protocols for enzyme activity assays using fluorogenic peptide substrates and sulfotransferase activity assays.

In Vitro Ubiquitination Protocols

Use this guide for detailed protocols describing how to carry out an in vitro ubiquitination reaction and analyze the results to determine if a specific protein is ubiquitinated, along with procedures for distinguishing between polyubiquitination and multi-monoubiquitination, and determining ubiquitin chain linkage.

 

 

Products for Glycobiology Research

Browse our tools for glycobiology research. We offer the largest selection of highly active enzymes for studying the structures and functions of specific glycoconjugates.

Modulate Enzymes with Small Molecules

Bio-Techne provides an extensive catalog of small molecule enzyme inhibitors, activators, and Degraders to help you explore enzyme activity. Our collection comprises potent and selective compounds targeting a wide range of different enzymes, including key enzymes in signaling pathways, the ubiquitin proteasome system, metabolism and more. 

 

 

Caspase Activation and Apoptosis Poster

Request a free copy of our Caspase Activation and Apoptosis poster, which highlights the domain structures of the different members of the caspase family of cysteine proteases and the pathways that lead to caspase activation and apoptosis.

 

Blood Coagulation Signaling Pathway

Explore our interactive Blood Coagulation Signaling Pathway to learn more about the serine proteases that function as coagulation factors and the other proteins involved in the extrinsic, intrinsic, and common pathways of blood coagulation.

Scientific Posters

Imaging Glycan Epitopes and Glycoproteins using Glycosyltransferases

Learn about a method for enzyme-based glycan imaging using glycosyltransferases that involves the incorporation of azido-sugars into target glycans followed by click chemistry-based conjugation with fluorescent reporters.

Phosphatase-Coupled Sulfotransferase Assay

See how this universal phosphatase-coupled sulfotransferase assay can be used to analyze the kinetics of sulfotransferases that use 3’-phosphoadenosine-5’-phosphosulfate (PAPS) as a donor substrate. The assay presented in this poster is both convenient and cost-effective as it eliminates the need for both radioisotope labeling and substrate-product separation.

 

 

 

Phosphatase-Coupled Universal Kinase Assay

Learn about a non-radioactive ADP-based phosphatase-coupled kinase assay that offers a new method for measuring kinase activity. The assay presented is direct and quantitative, requires no separation of product and substrate, and is compatible for high-throughput screening.

 

 

Background Information