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Simple Western Antibody Database
Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!
The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.
You can sort by column headings or just type into the Search box.
If you don't see your antibody listed here, shoot us an email at support@proteinsimple.com.
Simple Western Validated Antibodies
Filter Antibodies: (2858)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Host Species | Sample | Separation | Dilution | kDa on Simple Western | Matrix |
|---|---|---|---|---|---|---|---|---|---|
| α-Nucleocapsid | Primary |
Novus Biologicals NB100-56683 |
SARS Nucleocapsid Protein | Rabbit Polyclonal | Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) | Size | 25 mg/mL | 60 kDa | 12-230 kDa |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
14-3-3 gamma (γ) | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:50 | 33 kDa | 12-230 kDa |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
14-3-3 gamma (γ) | Rabbit Polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:10 | 34 kDa | 12-230 kDa |
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
14-3-3 gamma (γ) | Mouse Monoclonal | HeLa lysate 1.0 mg/ml | Size | 1:12.5 | 36 kDa | 12-230 kDa |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
14-3-3 zeta (ζ) | Mouse Monoclonal | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 kDa | 12-230 kDa |
| 15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
15-PGDH/HPGD | Goat Polyclonal | Human liver tissue | Size | 10 µg/mL | 34 kDa | 12-230 kDa |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
15-PGDH/HPGD | Rabbit polyclonal | Lovo (left) and HeLa (right) lysate 0.5 mg/ml | Size | 1:500 | 12-230 kDa | |
| 17 beta-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
17 beta-HSD1/HSD17B1 | Mouse Monoclonal | Human placenta tissue | Size | 2.5 µg/mL | 40 kDa | 12-230 kDa |
| 4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
4-Hydroxynonenal | Mouse Monoclonal | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | 12-230 kDa | |
| 4EBP1 | Primary |
R&D Systems AF3227 |
4EBP1 | Goat Polyclonal | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 kDa | 12-230 kDa |
| 5'-Nucleotidase/CD73 | Primary |
R&D Systems AF5795 |
5'-Nucleotidase/CD73 | Sheep Polyclonal | U266 human myeloma cell line | Size | 10 µg/mL | 86 | 12-230 kDa |
| 5-HT7 | Primary |
Novus Biologicals NB100-56352 |
5-Hydroxytryptamine receptor 7 | Rabbit Polyclonal | Rat Brain Lysate | Size | 20 µg/mL | 59 kDa | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58748 |
5-Lipoxygenase | Rabbit polyclonal | Human Lung lysate 0.5 mg/ml | Size | 50 µg/mL | 12-230 kDa | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58749 |
5-Lipoxygenase | Rabbit polyclonal | Human Lung lysate 0.5 mg/ml | Size | 10 µg/mL | 12-230 kDa | |
| 5HT1D | Primary |
Novus Biologicals NB100-56349 |
5HT1D | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:10 | 12-230 kDa | |
| 5HT3A | Primary |
Novus Biologicals NB100-56351 |
5HT3A | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:50 | 65 | 12-230 kDa |
| 6-His Epitope Tag | Primary |
Novus Biologicals NB100-64768 |
6-His Epitope Tag | Mouse monoclonal | rhEDG6 CHO S Tx lysate 0.1 mg/ml | Size | 1:250 | 12-230 kDa | |
| A. aeolicus BPL/BioID2 | Primary |
Novus Biologicals NBP2-59940 |
A. aeolicus BPL/BioID2 Antibody (SS QD1) | Mouse Monoclonal | Size | 94 kDa | |||
| A20/TNFAIP3 | Primary |
Novus Biologicals NBP1-77533 |
A20/TNFAIP3 | Mouse monoclonal | Jurkat lysate 0.05 mg/ml | Size | 1:100 | 102 | 12-230 kDa |
| A2BP1 | Primary |
Novus Biologicals NBP2-13169 |
A2BP1 | Mouse monoclonal | Human Brain lysate 0.5 mg/ml | Size | 1:20 | 54 | 12-230 kDa |
| ABCA1 | Primary |
Novus Biologicals NB400-105 |
ABCA1 | Rabbit polyclonal | Charge | 1:50 | |||
| ABCF2 | Primary |
Novus Biologicals NB400-115 |
ABCF2 | Rabbit polyclonal | HeLa lysate 0.05 mg/ml | Size | 1:1000 | 77 | 12-230 kDa |
| ABCG2 | Primary |
Novus Biologicals NBP1-59749 |
ABCG2 | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:50 | 72 | 12-230 kDa |
| Abhd5 | Primary |
Novus Biologicals NB110-41576 |
Abhd5 | Rabbit polyclonal | MCF-7 lysate 0.5 mg/ml | Size | 1:1000 | 55 | 12-230 kDa |
| ACADL | Primary |
Novus Biologicals NBP1-54936 |
ACADL Antibody | Rabbit Polyclonal | Rat/mouse liver | Size | 1:50 | 48 kDa | 12-230 kDa |
| ACE-2 | Primary |
R&D Systems AF3437 |
ACE-2 | Goat Polyclonal | Mouse kidney tissue | Size | 2.5 µg/mL | 141 | 12-230 kDa |
| ACE-2 | Primary |
R&D Systems AF933 |
ACE-2 | Goat Polyclonal | Human kidney tissue | Size | 10 µg/mL | 155 | 12-230 kDa |
| ACE-2 | Primary |
R&D Systems AF933 |
ACE-2 | Goat Polyclonal | Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) | Size | 1:50 | 158 | 12-230 kDa |
| ACE/CD143 | Primary |
R&D Systems AF1513 |
ACE/CD143 | Goat Polyclonal | Mouse lung tissue | Size | 10 µg/mL | 218 | 66-440 kDa |
| Acrp30 | Primary (biotinylated) |
R&D Systems BAF1065 |
Acrp30 | Goat | Human Serum | Size | 1:50 | 40 | 12-230 kDa |
| ACSF2 | Primary |
Novus Biologicals NBP2-47558 |
ACSF2 Antibody | Rabbit Polyclonal | Size | ||||
| ACSL1 | Primary |
Novus Biologicals NB110-99585 |
ACSL1 | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:100 | 65 | 12-230 kDa |
| ACSL3 | Primary |
Novus Biologicals NBP2-15252 |
ACSL3 Antibody | Rabbit Polyclonal | Mouse brain (hippocampus) tissue | Size | |||
| ACSL5 | Primary |
Novus Biologicals NBP1-59645 |
ACSL5 Antibody | Rabbit Polyclonal | Mouse liver lysate | Size | |||
| Actin | Primary |
Novus Biologicals NB100-74340 |
Actin | Mouse monoclonal | HeLa lysate 0.1 mg/ml | Size | 1:25 | 50 | 12-230 kDa |
| Actin Gamma 1 | Primary |
Novus Biologicals NB600-533 |
Actin Gamma 1 | Rabbit polyclonal | HeLa lysate 1.0 mg/ml | Size | 1:100 | 49 | 12-230 kDa |
| ADAMTS13 | Primary |
R&D Systems AF4245 |
Sheep Polyclonal | Size | 1:50-1:300 | 129, 217 | 12-230 kDa | ||
| ADARB1 | Primary |
Novus Biologicals NBP1-90240 |
ADARB1 | Rabbit Polyclonal | RT-4, U-251MG | Size | 1:20 | 94 | 12-230 kDa |
| ADE2 | Primary |
Novus Biologicals NBP1-90235 |
ADE2 | Rabbit Polyclonal | RT-4 and U-251MG | Size | 1:20 | 47/50 | 12-230 kDa |
| Adenosine A2a R | Primary |
Novus Biologicals NB300-597 |
Adenosine A2a R | Rabbit polyclonal | HeLa lysate 0.5 mg/ml | Size | 1:20 | 51 | 12-230 kDa |
| ADFP | Primary |
Novus Biologicals NB110-40877 |
ADFP | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:50 | 77 | 12-230 kDa |
| ADFP | Primary |
Novus Biologicals NB110-40878 |
ADFP | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:50 | 46 | 12-230 kDa |
| ADI1 | Primary |
Novus Biologicals NBP2-38251 |
ADI1 | Rabbit Polyclonal | HepG2 lysate | Size | 1:25 | 26 | 12-230 kDa |
| Adiponectin/Acrp30 | Primary |
R&D Systems AF1065 |
Adiponectin/Acrp30 | Goat Polyclonal | Human placenta tissue | Size | 10 µg/mL | 41 | 12-230 kDa |
| AGR2 | Primary |
Novus Biologicals NBP2-27393 |
AGR2 | Rabbit polyclonal | MCF-7 lysate 0.5 mg/ml | Size | 1:50 | 22 | 12-230 kDa |
| AGR2 | Primary |
Novus Biologicals NBP2-27392 |
AGR2 | Rabbit polyclonal | MCF-7 lysate 0.5 mg/ml | Size | 1:100 | 27 | 12-230 kDa |
| AGXT | Primary |
Novus Biologicals NBP1-89200 |
AGXT | Rabbit Polyclonal | Liver (left), HepG2 (right) | Size | 1:20 | 47 | 12-230 kDa |
| AHR | Primary |
R&D Systems AF6185 |
AHR | Sheep Polyclonal | PC-3 human prostate cancer cell line | Size | 10 µg/mL | 109 | 12-230 kDa |
| AIF | Primary |
Novus Biologicals NBP2-27177 |
AIF | Rabbit polyclonal | Jurkat and MCF-7 lysate 0.2 mg/ml | Size | 1:500 | 12-230 kDa | |
| AIF | Primary |
R&D Systems AF1457 |
AIF | Rabbit Polyclonal | Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line | Size | 1 µg/mL | 69 | 12-230 kDa |
