Simple Western Antibody Database

Simple Western Certified Antibodies from R&D Systems, Novus Biologicals and More!

The Simple Western antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. The performance of any listed antibody will need to be optimized for the biological system being examined.

You can sort by column headings or just type into the Search box.

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Simple Western Validated Antibodies

Filter Antibodies: (3858)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-Nucleocapsid Primary Novus Biologicals
NB100-56683 		SARS Nucleocapsid Protein Datasheet Rabbit Polyclonal Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60 kDa
14-3-3 γ Primary Novus Biologicals
NB100-406 		14-3-3 gamma (γ) Datasheet Mouse Monoclonal HeLa lysate 1.0 mg/ml Size 1:12.5 36 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27202 		14-3-3 gamma (γ) Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 33 kDa
14-3-3 γ Primary Novus Biologicals
NBP2-27370 		14-3-3 gamma (γ) Datasheet Rabbit Polyclonal Human Brain lysate 0.5 mg/ml Size 1:10 34 kDa
14-3-3 ζ Primary R&D Systems
MAB2669 		14-3-3 zeta (ζ) Datasheet Mouse Monoclonal SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34 kDa
15-PGDH/HPGD Primary Novus Biologicals
NB200-179 		15-PGDH/HPGD Datasheet Rabbit polyclonal Lovo (left) and HeLa (right) lysate 0.5 mg/ml Size 1:500
15-PGDH/HPGD Primary R&D Systems
AF5660 		15-PGDH/HPGD Datasheet Goat Polyclonal Human liver tissue Size 10 µg/mL 34 kDa
17 beta-HSD1/HSD17B1 Primary R&D Systems
MAB7178 		17 beta-HSD1/HSD17B1 Datasheet Mouse Monoclonal Human placenta tissue Size 2.5 µg/mL 40 kDa
4-Hydroxynonenal Primary R&D Systems
MAB3249 		4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4EBP1 Primary R&D Systems
AF3227 		4EBP1 Datasheet Goat Polyclonal HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26 kDa
5'-Nucleotidase/CD73 Primary R&D Systems
AF5795 		5'-Nucleotidase/CD73 Datasheet Sheep Polyclonal U266 human myeloma cell line Size 10 µg/mL 86
5-HT7 Primary Novus Biologicals
NB100-56352 		5-Hydroxytryptamine receptor 7 Datasheet Rabbit Polyclonal Rat Brain Lysate Size 20 µg/mL 59 kDa
5-Lipoxygenase Primary Novus Biologicals
NB110-58748 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 50 µg/mL
5-Lipoxygenase Primary Novus Biologicals
NB110-58749 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 10 µg/mL
5HT1D Primary Novus Biologicals
NB100-56349 		5HT1D Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:10
5HT3A Primary Novus Biologicals
NB100-56351 		5HT3A Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 65
6-His Epitope Tag Primary Novus Biologicals
NB100-64768 		6-His Epitope Tag Datasheet Mouse monoclonal rhEDG6 CHO S Tx lysate 0.1 mg/ml Size 1:250
A. aeolicus BPL/BioID2 Primary Novus Biologicals
NBP2-59940 		A. aeolicus BPL/BioID2 Antibody (SS QD1) Datasheet Mouse Monoclonal Size 94 kDa
A20/TNFAIP3 Primary Novus Biologicals
NBP1-77533 		A20/TNFAIP3 Datasheet Mouse monoclonal Jurkat lysate 0.05 mg/ml Size 1:100 102
A2BP1 Primary Novus Biologicals
NBP2-13169 		A2BP1 Datasheet Mouse monoclonal Human Brain lysate 0.5 mg/ml Size 1:20 54
ABCA1 Primary Novus Biologicals
NB400-105 		ABCA1 Datasheet Rabbit polyclonal Charge 1:50
ABCF2 Primary Novus Biologicals
NB400-115 		ABCF2 Datasheet Rabbit polyclonal HeLa lysate 0.05 mg/ml Size 1:1000 77
ABCG2 Primary Novus Biologicals
NBP1-59749 		ABCG2 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 72
Abhd5 Primary Novus Biologicals
NB110-41576 		Abhd5 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:1000 55
ACADL Primary Novus Biologicals
NBP1-54936 		ACADL Antibody Datasheet Rabbit Polyclonal Rat/mouse liver Size 1:50 48 kDa
ACE-2 Primary R&D Systems
AF933 		ACE-2 Datasheet Goat Polyclonal Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) Size 1:50 158
ACE-2 Primary R&D Systems
AF3437 		ACE-2 Datasheet Goat Polyclonal Mouse kidney tissue Size 2.5 µg/mL 141
ACE-2 Primary R&D Systems
AF933 		ACE-2 Datasheet Goat Polyclonal Human kidney tissue Size 10 µg/mL 155
ACE/CD143 Primary R&D Systems
AF1513 		ACE/CD143 Datasheet Goat Polyclonal Mouse lung tissue Size 10 µg/mL 218
Acrp30 Primary (biotinylated) R&D Systems
BAF1065 		Acrp30 Datasheet Goat Human Serum Size 1:50 40
ACSF2 Primary Novus Biologicals
NBP2-47558 		ACSF2 Antibody Datasheet Rabbit Polyclonal Size
ACSL1 Primary Novus Biologicals
NB110-99585 		ACSL1 Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:100 65
ACSL3 Primary Novus Biologicals
NBP2-15252 		ACSL3 Antibody Datasheet Rabbit Polyclonal Mouse brain (hippocampus) tissue Size
ACSL5 Primary Novus Biologicals
NBP1-59645 		ACSL5 Antibody Datasheet Rabbit Polyclonal Mouse liver lysate Size
Actin Primary Novus Biologicals
NB100-74340 		Actin Datasheet Mouse monoclonal HeLa lysate 0.1 mg/ml Size 1:25 50
Actin Gamma 1 Primary Novus Biologicals
NB600-533 		Actin Gamma 1 Datasheet Rabbit polyclonal HeLa lysate 1.0 mg/ml Size 1:100 49
ADAMTS13 Primary R&D Systems
AF4245 		Datasheet Sheep Polyclonal Size 1:50-1:300 129, 217
ADARB1 Primary Novus Biologicals
NBP1-90240 		ADARB1 Datasheet Rabbit Polyclonal RT-4, U-251MG Size 1:20 94
ADE2 Primary Novus Biologicals
NBP1-90235 		ADE2 Datasheet Rabbit Polyclonal RT-4 and U-251MG Size 1:20 47/50
Adenosine A2a R Primary Novus Biologicals
NB300-597 		Adenosine A2a R Datasheet Rabbit polyclonal HeLa lysate 0.5 mg/ml Size 1:20 51
ADFP Primary Novus Biologicals
NB110-40877 		ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 77
ADFP Primary Novus Biologicals
NB110-40878 		ADFP Datasheet Rabbit polyclonal HepG2 lysate 0.5 mg/ml Size 1:50 46
ADI1 Primary Novus Biologicals
NBP2-38251 		ADI1 Datasheet Rabbit Polyclonal HepG2 lysate Size 1:25 26
Adiponectin/Acrp30 Primary R&D Systems
AF1065 		Adiponectin/Acrp30 Datasheet Goat Polyclonal Human placenta tissue Size 10 µg/mL 41
AGR2 Primary Novus Biologicals
NBP2-27392 		AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:100 27
AGR2 Primary Novus Biologicals
NBP2-27393 		AGR2 Datasheet Rabbit polyclonal MCF-7 lysate 0.5 mg/ml Size 1:50 22
AGXT Primary Novus Biologicals
NBP1-89200 		AGXT Datasheet Rabbit Polyclonal Liver (left), HepG2 (right) Size 1:20 47
AHR Primary R&D Systems
AF6185 		AHR Datasheet Sheep Polyclonal PC-3 human prostate cancer cell line Size 10 µg/mL 109
AIF Primary Novus Biologicals
NBP2-27177 		AIF Datasheet Rabbit polyclonal Jurkat and MCF-7 lysate 0.2 mg/ml Size 1:500
AIF Primary R&D Systems
AF1457 		AIF Datasheet Rabbit Polyclonal Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line Size 1 µg/mL 69
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