Human 17 beta-HSD1/HSD17B1 Antibody

Name: Human 17 beta-HSD1/HSD17B1 Antibody
Brand: rnd
SKU: MAB7178
Rating: 4 (1 reviews)

Catalog # MAB7178

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human 17 beta ‑HSD1/HSD17B1 Ala2-Gln328 Accession # P14061
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 860020
Specificity:	Detects human 17 beta ‑HSD1/HSD17B1 in ELISAs.

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Applications

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human 17 beta-HSD1/HSD17B1 Antibody Scientific Data Examples

	Western Blot Detection of Human 17 beta ‑HSD1/ HSD17B1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human 17 beta -HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 17 beta -HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human 17 beta ‑HSD1/HSD17B1 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for 17 beta ‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human 17 beta ‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17 beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

Item	Value
Long Name	17 beta Hydroxysteroid Dehydrogenase 1
Entrez Gene IDs	3292 (Human); 15485 (Mouse); 25322 (Rat)
Alternate Names	17 beta-HSD1, 17 betaHSD1, 17-beta-HSD 1, 17-beta-hydroxysteroid dehydrogenase type 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E17KSR, E2DH, EC 1.1.1.62, EDH17B1, EDH17B2, EDH17B2EDHB17, EDHB17, HSD17, HSD17B1, MGC138140, Placental 17-beta-hydroxysteroid dehydrogenase, SDR28C1, estradiol 17-beta-dehydrogenase 1, estradiol 17-beta-dehydrogenase-1, hydroxysteroid (17-beta) dehydrogenase 1, hydroxysteroid (17-beta) dehydrogenase 1 isoform, short chain dehydrogenase/reductase family 28CE, member 1

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Human 17 beta -HSD1/HSD17B1 Antibody

Name: Anonymous

Application: WB

Sample Tested: Endometrial epithelial

Species: Human

Verified Customer | Posted 03/27/2017

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