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Simple Western Instruments and Consumables

Jess Instrument

  • Size-based automated capillary Western blot assays
  • Up to 25 samples per run
  • Includes RePlex™ and Stellar™
  • Chemiluminescence and fluorescence detection
Abby Instrument

  • Size-based automated capillary Western blot assays
  • Up to 25 samples per run
  • Includes RePlex™
  • Chemiluminescence detection
Peggy Sue instrument by ProteinSimple a Bio-Techne brand

  • Size and charge-based automated capillary Western blot assays
  • Up to 96 samples per run
  • Chemiluminescence detection
Sally Sue instrument by ProteinSimple a Bio-Techne brand

  • Size-based automated capillary Western blot assays
  • Up to 96 samples per run
  • Chemiluminescence detection
NanoPro instrument by ProteinSimple a Bio-Techne brand

  • Charge-based automated capillary Western blot assays
  • Up to 96 samples per run
  • Chemiluminescence detection

Wes™ has been discontinued as of July 30th, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026.
Upgrade to Jess or Abby to have all the functionality of Wes with additional features like RePlex™ and Stellar™.

What Is Simple Western?

What is Simple Western?

Simple Western is a next-generation hands-free capillary immunoassay platform combining protein separation with sensitive chemiluminescence and fluorescence immunodetection using conventional primary and conjugated secondary antibodies.

  • Simple Western is the only fully automated Western device on the market. Traditional Western blotting steps of sample separation, wash buffer incubations, and immunodetection of target proteins occur inside a hands-free capillary instrument on your benchtop.
  • Unlike traditional Western blotting systems, Simple Western does not transfer proteins to a membrane, removing the inherent variability of Western blotting and adding high-throughput sample processing and automated protein analysis.
  • Simple Western separates tiny amounts of samples (<1 µg lysate/well) inside capillaries and cross-links your samples to the capillary walls. Then, Simple Western performs blocking and wash buffer incubations and uses just a few microliters of your primary antibodies and conjugated secondary antibodies to detect target proteins in chemiluminescence and NIR/IR fluorescence channels (LOD <1 pg).

How Can Simple Western Help You?

Fully Automated. Just load your samples and press start. Pair your Simple Western instrument with your automated liquid handler to prepare and load samples, reagents, antibodies, and wash buffer, and set up multiple plates for automated protein analysis. 

Low Sample Volume. Use as little as 3 µL of starting material. Researchers can generate up to 8 data points with just 5 µL.

High Sensitivity Multiplex. Get industry-leading sensitivity in chemiluminescence and fluorescence channels and the advantage of flexible multiplex assays.

Fast Time to Results. With a processing time of 3 hours, Simple Western dramatically speeds up time to results compared to other Western blot processors.

Reproducible and Quantitative. By eliminating the variability from the gel-to-membrane transfer of Western blotting protocols, Simple Western is highly reproducible and fully quantitative. Plus, you get total protein detection and built-in total protein normalization.

Reduce Costs. RePlex™ on Jess and Abby lowers the cost per result compared to other Western blot processors. Save samples, reagents, time, and labor.

Jess vs Western Blotting Infographic

Simple Western Versus ELISA

Simple Western vs traditional ELISA workflow - Benzonase assay

Reduced Matrix Effects

Simple Western overcomes matrix effects of complex samples that can interfere with your ELISA and flow cytometry results.

Simpler Assay Development

Only one target-specific antibody is required. Thousands of validated antibodies or validate a new antibody

Plus, Protein Size Separation

Get protein size information to quantify target protein isoforms, fragments, aggregates, degradation products, and more.

 

Researchers Reveal Why They Use Simple Western

Researchers explain why they use our Simple Western next-generation immunoassay systems for automated protein analysis.

Stellar and RePlex Modules

High-Sensitivity Multiplexing

Stellar™ NIR/IR Detection Modules provide industry-leading fluorescence sensitivity at detection levels below one pg, excellent reproducibility, and a four-log dynamic range. Stellar fluorescence detection rivals the widely recognized sensitivity of chemiluminescence detection and is 100X more sensitive than the closest competing fluorescent Western blotting imager.

 

Run Two Immunoassays in the Same Capillary

Perform sequential immunoassays in the same capillary with RePlex™. Unlike the strip and reprobe method of Western blotting membranes, RePlex completely removes primary antibodies and secondary antibodies from the capillary without losing your immobilized protein sample, allowing for excellent reproducibility between probing cycles. With RePlex assays on Simple Western, researchers leave behind the laborious and unreliable strip and reprobe method of Western blotting.

Stellar Versus RePlex