How Can Simple Western Help You?
Low Sample Volume
Simple Western systems use as little as 3 µL of starting material and give down to picogram-level sensitivity. Up to 8 data points can be generated out of just 5 µL, allowing you to get the most out of your precious sample.
Automation
Simple Western assays are fully automated following a simple sample preparation, with no gels, no transfer devices, no blots, no film and no manual analysis. Just load your samples and press start—it's a complete, walk-away solution for protein separation and detection.
Reproducible
Due to the elimination of variability inherent to manual processes, Simple Western assays are highly reproducible, while adding quantitation, and improving on time-to-result and overall data reliability.
Fast Time to Results
Up to 96 samples can be processed at once, and Simple Western assays take just 3-19 hours to complete, all in a fully-automated, walk-away fashion.
Separate Proteins by Size or Charge
Simple Western systems offer two ways to separate proteins:
RePlex™
Perform sequential immunoassays in the same capillary using RePlex™ with Jess™ and eliminate the strip-and-reprobe associated with traditional Western blots. Click here to learn more about RePlex with Jess.
Find out more about how Simple Western technology works at ProteinSimple.com
Peggy Sue
Peggy Sue
Assay Type: Size and charge assays on up to 96 samples
Detection Mode: Chemiluminescence
Sally Sue
Sally Sue
Assay Type: Size assays on up to 96 samples
Detection Mode: Chemiluminescence
NanoPro 1000
NanoPro 1000
Assay Type: Charge assays on up to 96 samples
Detection Mode: Chemiluminescence
Which system is right for you?
Simple Western System |
Jess |
Wes |
Sally Sue |
Peggy Sue |
NanoPro 1000 |
|---|---|---|---|---|---|
Simple Western size assays |
Yes |
Yes |
Yes |
Yes |
No |
Simple Western charge assays |
No |
No |
No |
Yes |
Yes |
Chemiluminescence |
Yes |
Yes |
Yes |
Yes |
Yes |
Fluorescence |
Yes |
No |
No |
No |
No |
Maximum samples per run |
25 |
25 |
96 |
96 |
96 |
Run time for max samples (size) |
<3 hours |
<3 hours |
14-19 hours |
14-19 hours |
N/A |
Run time for max samples (charge) |
N/A |
N/A |
N/A |
11 hours |
11 hours |
Sample cooling (size) |
N/A |
N/A |
10ºC |
10ºC |
N/A |
Sample cooling (charge) |
N/A |
N/A |
N/A |
3ºC |
3ºC |
Dimensions (H x W x D) |
33 x 33 x 52 cm |
33 x 33 x 52 cm |
94 x 94 x 61 cm |
94 x 94 x 61 cm |
94 x 94 x 61 cm |
Part number |
004-650 |
004-600 |
004-700 |
004-900 |
004-109 |
Immunoassay Size Specification |
Immunoassay Size Specification |
Immunoassay Charge Specification |
|
|---|---|---|---|
Description |
Chemiluminescence |
Fluorescence |
Chemiluminescence |
Sample required |
0.3-1.2 µg lysate |
0.6-1.2 µg |
0.6-1.2 µg |
Size or pI range |
2-40, 12-230 and 66-440 kDa |
2-40, 12-230 and 66-440 kDa |
Widest gradient ranges from pI 3 to pI 10 |
Sizing CV |
<10% |
<10% |
<10% |
Intra-assay CV |
<15% |
<15% |
<20% |
Inter-assay CV |
<20%* |
<20%* |
<20%** |
Resolution (± percent difference in MW) |
±15-20% for MW <20 kDa ±10% for MW >20 kDa |
±15-20% for MW <20 kDa ±10% for MW >20 kDa |
±0.1 pI units |
Quantitation CV |
<20% |
<20% |
<20% |
Dynamic range |
2-3 logs |
up to 4 logs*** |
3 logs |
Sensitivity |
ng |
Low pg |
Low pg |
Capillary |
5 cm, 100 µm, 400 nL |
5 cm, 100 µm, 400 nL |
5 cm, 100 µm, 400 nL |
