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How Can Simple Western Help You?

Low Sample Volume

Simple Western systems use as little as 3 µL of starting material and give down to picogram-level sensitivity. Up to 8 data points can be generated out of just 5 µL, allowing you to get the most out of your precious sample. 

Automation

Simple Western assays are fully automated following a simple sample preparation, with no gels, no transfer devices, no blots, no film and no manual analysis. Just load your samples and press start—it's a complete, walk-away solution for protein separation and detection.

Reproducible

Due to the elimination of variability inherent to manual processes, Simple Western assays are highly reproducible, while adding quantitation, and improving on time-to-result and overall data reliability.

Fast Time to Results

Up to 96 samples can be processed at once, and Simple Western assays take just 3-19 hours to complete, all in a fully-automated, walk-away fashion. 

Separate Proteins by Size or Charge

Simple Western systems offer two ways to separate proteins:

  1. Size-based separation assays
  2. Charge-based separation assays

RePlex™

Perform sequential immunoassays in the same capillary using RePlex™ with Jess™ and eliminate the strip-and-reprobe associated with traditional Western blots. Click here to learn more about RePlex with Jess.

Find out more about how Simple Western technology works at ProteinSimple.com

Simple Western Instruments

Jess Simple Western instrument by ProteinSimple, a Bio-Techne brand

Jess

Assay Type: Size assays on up to 25 samples

Detection Modes: Chemiluminescence and fluorescence

Wes Simple Western instrument by ProteinSimple, a Bio-Techne brand

Wes

Assay Type: Size assays on up to 25 samples

Detection Mode: Chemiluminescence

Peggy Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Peggy Sue

Assay Type: Size and charge assays on up to 96 samples

Detection Mode: Chemiluminescence

Sally Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Sally Sue

Assay Type: Size assays on up to 96 samples

Detection Mode: Chemiluminescence

Nanopro 1000 Simple Western instrument ProteinSimple, a Bio-Techne brand

NanoPro 1000

Assay Type: Charge assays on up to 96 samples

Detection Mode: Chemiluminescence

Which system is right for you?

All systems operate at 20-60% relative, non-condensing humidity, a temperature range of 18-24ºC, and a power range of 100-230 V
Simple Western System

Jess

Wes

Sally Sue

Peggy Sue

NanoPro 1000

Simple Western size assays

Yes

Yes

Yes

Yes

No

Simple Western charge assays

No

No

No

Yes

Yes

Chemiluminescence

Yes

Yes

Yes

Yes

Yes

Fluorescence

Yes

No

No

No

No

Maximum samples per run

25

25

96

96

96

Run time for max samples (size)

<3 hours

<3 hours

14-19 hours

14-19 hours

N/A

Run time for max samples (charge)

N/A

N/A

N/A

11 hours

11 hours

Sample cooling (size)

N/A

N/A

10ºC

10ºC

N/A

Sample cooling (charge)

N/A

N/A

N/A

3ºC

3ºC

Dimensions (H x W x D)

33 x 33 x 52 cm

33 x 33 x 52 cm

94 x 94 x 61 cm

94 x 94 x 61 cm

94 x 94 x 61 cm

Part number

004-650

004-600

004-700

004-900

004-109

*inter-assay CV is with system control, **percent peak area, ***Jess/Wes with HDR detection mode.

Immunoassay Size Specification

Immunoassay Size Specification

Immunoassay Charge Specification

Description

Chemiluminescence

Fluorescence

Chemiluminescence

Sample required

0.3-1.2 µg lysate

0.6-1.2 µg

0.6-1.2 µg

Size or pI range

2-40, 12-230 and 66-440 kDa

2-40, 12-230 and 66-440 kDa

Widest gradient ranges from pI 3 to pI 10

Sizing CV

<10%

<10%

<10%

Intra-assay CV

<15%

<15%

<20%

Inter-assay CV

<20%*

<20%*

<20%**

Resolution (± percent difference in MW)

±15-20% for MW <20 kDa

±10% for MW >20 kDa

±15-20% for MW <20 kDa

±10% for MW >20 kDa

±0.1 pI units

Quantitation CV

<20%

<20%

<20%

Dynamic range

2-3 logs

up to 4 logs***

3 logs

Sensitivity

ng

Low pg

Low pg

Capillary

5 cm, 100 µm, 400 nL

5 cm, 100 µm, 400 nL

5 cm, 100 µm, 400 nL

Simple Western Applications

Simple Western applications in cancer research

Cancer

Advanced Western Blotting Solutions for Cancer Research