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ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple.
How Can Simple Western Help You?
Low Sample Volume
Simple Western systems use as little as 3 µL of starting material and give down to picogram-level sensitivity. Up to 8 data points can be generated out of just 5 µL, allowing you to get the most out of your precious sample.
Automation
Simple Western assays are fully automated following a simple sample preparation, with no gels, no transfer devices, no blots, no film and no manual analysis. Just load your samples and press start—it's a complete, walk-away solution for protein separation and detection.
Reproducible
Due to the elimination of variability inherent to manual processes, Simple Western assays are highly reproducible, while adding quantitation, and improving on time-to-result and overall data reliability.
Fast Time to Results
Up to 96 samples can be processed at once, and Simple Western assays take just 3-19 hours to complete, all in a fully-automated, walk-away fashion.
Separate Proteins by Size or Charge
Simple Western systems offer two ways to separate proteins:
RePlex™
Perform sequential immunoassays in the same capillary using RePlex™ with Jess™ or Abby™ and eliminate the strip-and-reprobe associated with traditional Western blots. Click here to learn more about RePlex with Jess or Abby.
Simple Western Instruments
Peggy Sue
Peggy Sue
Assay Type: Size and charge assays on up to 96 samples
Detection Mode: Chemiluminescence
Sally Sue
Sally Sue
Assay Type: Size assays on up to 96 samples
Detection Mode: Chemiluminescence
NanoPro 1000
NanoPro 1000
Assay Type: Charge assays on up to 96 samples
Detection Mode: Chemiluminescence
Which system is right for you?
Simple Western System |
Jess |
Abby |
Wes |
Sally Sue |
Peggy Sue |
NanoPro 1000 |
|---|---|---|---|---|---|---|
Simple Western size assays |
Yes | Yes | Yes | Yes | Yes | No |
Simple Western charge assays |
No | No | No | No | Yes | Yes |
Chemiluminescence |
Yes | Yes | Yes | Yes | Yes | Yes |
Fluorescence |
Yes | No | No | No | No | No |
Includes RePlex for sequential immunoassays (or IA + Total Protein) with the same sample |
Yes | Yes | No | No | No | No |
Maximum samples per run |
25 | 25 | 25 | 96 | 96 | 96 |
Run time for max samples (size) |
<3 hours <5 hours with RePlex |
<3 hours <5 hours with RePlex |
<3 hours | 14-19 hours | 14-19 hours | N/A |
Run time for max samples (charge) |
N/A | N/A | N/A | N/A | 11 hours | 11 hours |
Sample cooling (size) |
N/A | N/A | N/A | 10ºC | 10ºC | N/A |
Sample cooling (charge) |
N/A | N/A | N/A | N/A | 3ºC | 3ºC |
Dimensions (H x W x D) |
33 x 33 x 52 cm | 33 x 33 x 52 cm | 33 x 33 x 52 cm | 94 x 94 x 61 cm | 94 x 94 x 61 cm | 94 x 94 x 61 cm |
Part number |
004-650 | 004-680 | 004-600 | 004-700 | 004-900 | 004-109 |
Immunoassay Size Specification |
Immunoassay Size Specification |
Immunoassay Charge Specification |
|
|---|---|---|---|
Description |
Chemiluminescence |
Fluorescence |
Chemiluminescence |
Sample required |
0.3-1.2 µg lysate |
0.6-1.2 µg |
0.6-1.2 µg |
Size or pI range |
2-40, 12-230 and 66-440 kDa |
2-40, 12-230 and 66-440 kDa |
Widest gradient ranges from pI 3 to pI 10 |
Sizing CV |
<10% |
<10% |
<10% |
Intra-assay CV |
<15% |
<15% |
<20% |
Inter-assay CV |
<20%* |
<20%* |
<20%** |
Resolution (± percent difference in MW) |
±15-20% for MW <20 kDa ±10% for MW >20 kDa |
±15-20% for MW <20 kDa ±10% for MW >20 kDa |
±0.1 pI units |
Quantitation CV |
<20% |
<20% |
<20% |
Dynamic range |
2-3 logs |
up to 4 logs*** |
3 logs |
Sensitivity |
ng |
Low pg |
Low pg |
Capillary |
5 cm, 100 µm, 400 nL |
5 cm, 100 µm, 400 nL |
5 cm, 100 µm, 400 nL |
Simple Western Applications
Cancer & Immuno-Oncology
Cancer & Immuno-Oncology
Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
SARS‑CoV‑2
SARS‑CoV‑2
Reduce your lab time with automated simple western systems while accelerating your COVID‑19 research. Set up, leave and remotely review your data in just 3 hours!
Cell & Gene Therapy
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Targeted Degradation
Targeted Degradation
Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.
SARS-CoV-2 Serology
SARS-CoV-2 Serology
Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!
Exosome
Exosome
A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.
Vaccine Development
Vaccine Development
Improve reproducibility, quantitation, and time to market for your vaccine candidates.
Neuroscience
Neuroscience
Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.
