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Simple Western Instruments and Consumables

Jess Instrument

Jess™

  • Size-based automated capillary Western blot assays
  • Up to 25 samples
  • Includes RePlex™ and Stellar™
  • Chemiluminescence and fluorescence
Abby Instrument

Abby™

  • Size-based automated capillary Western blot assays
  • Up to 25 samples
  • Includes RePlex™
  • Chemiluminescence
Peggy Sue instrument by ProteinSimple a Bio-Techne brand

Peggy Sue™

  • Size and charge-based automated capillary Western blot assays
  • Up to 96 samples
  • Chemiluminescence
Sally Sue instrument by ProteinSimple a Bio-Techne brand

Sally Sue™

  • Size-based automated capillary Western blot assays
  • Up to 96 samples
  • Chemiluminescence
NanoPro instrument by ProteinSimple a Bio-Techne brand

NanoPro™ 1000

  • Charge-based automated capillary Western blot assays
  • Up to 96 samples
  • Chemiluminescence

Wes™ has been discontinued as of July 30th, 2021. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Upgrade to Jess or Abby to have all the functionality of Wes with additional features like RePlex and Stellar High Sensitivity NIR/IR Detection (Jess only).

What is Simple Western?

Simple Western is a next-generation biomolecular analytical tool that seamlessly combines capillary electrophoresis with immunodetection using conventional primary antibodies and conjugated secondary antibodies.

  • Simple Western is the only fully automated Western blot processor on the market. Traditional Western blotting steps and incubations, like sample separation, blocking, wash buffer incubations, incubations of primary and secondary antibodies, and detection of target proteins are precisely controlled inside a hands-free capillary electrophoresis instrument.
  • Unlike traditional Western blots, separated proteins are covalently attached to the capillary wall, eliminating the need to transfer proteins to a Western blotting membrane.

Separate Proteins by Size or by Charge

  • Simple Western Size. Size-based capillary Western blot assays use capillary electrophoresis sodium dodecyl sulfate (CE-SDS) to separate proteins by molecular weight up to 440 kDa. Analyze small polypeptides or large target proteins like dystrophin.
  • Simple Western Charge. Charge-based capillary Western blot assays use capillary isoelectric focusing (cIEF) to separate proteins by isoelectric point. Resolve protein isoforms like phospho-proteins with high resolution. 
Simple Western Size and Charge Separation Protein Assays Figure

How Can Simple Western Help You?

Fully Automated. Just load your samples, press start, and come back to analyzed results. You can even pair your Simple Western instrument with an automated liquid handler to prepare and load samples, reagents, antibodies, and wash buffer, and set up multiple plates for analysis. Check out the Simple Western FeliX from Analytik Jena for one example on how Simple Western pairs with liquid handling automation.

Low Sample Volume. Use as little as 3 µL of starting material. Generate up to 8 data points with just 5 µL.

High Sensitivity Multiplex. With Jess, researchers get industry-leading sensitivity in chemiluminescence and fluorescence channels and the advantage of flexible multiplex analysis.

Fast Time to Results. With a processing time of 3 hours for 25 samples or 96 samples overnight, Simple Western dramatically speeds up time to results compared to traditional Western blotting protocols.

Reproducible and Quantitative. By eliminating the variability of traditional Western blotting protocols like the gel-to-membrane transfer, Simple Western assays are highly reproducible and fully quantitative. Researchers get accurate measurements of target proteins with Total Protein Assays and built-in Total Protein Normalization.

Reduce Costs. RePlex™ on Jess and Abby lowers the cost per result compared to traditional Western blotting protocols. With Simple Western, researchers save samples, reagents, wash buffer, time, and labor!

More Than Just a Western Blot Alternative. Featured in well over 1500 publications and rapidly growing, Simple Western is a proven technology in all types of research applications, going far beyond what can be done with traditional Western blotting. See what other researchers are saying about Simple Western!

Jess vs Western Blotting Infographic

Stellar Modules: Industry-Leading Fluorescence Detection Sensitivity

Stellar™ NIR/IR Detection Modules on Jess provide industry-leading fluorescence sensitivity at detection levels below 1 pg, along with excellent reproducibility and a 4-log dynamic range. With this leap in sensitivity, Stellar fluorescence detection rivals the widely recognized sensitivity of chemiluminescence detection and demolishes the fluorescence detection of the closest competing traditional Western blot imaging instrument, which requires at least 50 pg.

Webinar hero image with an artistic depiction of the Stellar technology

ReThink, ReUse, RePlex

Perform sequential immunoassays in the same capillary using RePlex™ assays on Jess or Abby. Save time and money and boost your productivity with our automated Western blot processors! RePlex efficiently removes primary antibodies and secondary antibodies between probing cycles because samples are covalently immobilized, allowing for excellent reproducibility across cycles. With channel-based multiplexing, you can detect multiple target proteins per cycle. The second cycle can also be dedicated total protein detection to accurately normalize protein expression data.

Jess, Abby and Replex GIF

Which Simple Western System is Right for You?

  Jess Abby Sally Sue Peggy Sue NanoPro 1000

Size assays

Yes

Yes

Yes

Yes

No

Charge assays

No

No

No

Yes

Yes

Chemiluminescence

Yes

Yes

Yes

Yes

Yes

Fluorescence

Yes

No

No

No

No

Stellar

Yes No No No No

RePlex

Yes

Yes

No

No

No

Max samples per run

25

25

96

96

96

Max run time

<3 hours, <5 hours with RePlex

<3 hours, <5 hours with RePlex

14-19 hours

14-19 hours (size), 11 hours (charge)

11 hours

Sample cooling

N/A

N/A

10 ºC

10 ºC (size), 3 ºC (charge)

3 ºC

Dimensions (H x W x D)

33 x 33 x 52 cm

33 x 33 x 52 cm

94 x 94 x 61 cm

94 x 94 x 61 cm

94 x 94 x 61 cm

Part number

004-650

004-680

004-700

004-900

004-109

All systems operate at 20-60% relative, non-condensing humidity, a temperature range of 18-24 ºC, and a power range of 100-230 V

 

Simple Western Specifications

 

Total Protein

Immunoassay (Size)

Immunoassay (Charge)

Sample required

0.3-1.2 µg lysate

0.6-1.2 µg lysate

0.6-1.2 µg

Size or pI range

2-40, 12-230, or 66-440 kDa

2-40, 12-230, or 66-440 kDa

pI 3 to pI 10

Sizing CV

<10%

<10%

<10%

Intra-assay CV

<15%

<15%

<20%

Inter-assay CV

<20%

<20%

<20%

Resolution

±15-20% <20 kDa, ±10% >20 kDa

±15-20% <20 kDa, ±10% >20 kDa

±0.1 pI units

Quantitation CV

<20%

<20%

<20%

Dynamic range

2-3 logs

up to 4 logs

3 logs

Sensitivity

Mid pg with 5X Total Protein

Low pg

Low pg

Capillary

5 cm, 100 µm, 400 nL

5 cm, 100 µm, 400 nL

5 cm, 100 µm, 400 nL

Simple Western Applications

immune cells attack cancer cell

Immuno-Oncology

 

Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.

Solutions for Cell Signaling and Signal Pathways Analysis

Cell Signaling

 

Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization.

Testing for Lentivirus and AAVs

Cell and Gene Therapy

 

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Advances in Targeted Protein Degradation

Targeted Degradation

 

Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

Biosimilar Antibodies Teaser Image

Pharmacokinetic Analysis

 

High-throughput pharmacokinetic analysis with quantitative, high-sensitivity assays directly in complex lysates and tissue homogenates. 

Exosomes

Exosomes

 

A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity Western blotting solutions.

Vaccine development image

Vaccine Development

 

Improve reproducibility, quantitation, and time to market for your vaccine candidates.

Neuroscience App image for Ella

Neuroscience

 

Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.

Simple Plex HEK-HCP Assay

Bioprocess Impurities

 

Screen for processing-related impurities and host-cell related contaminants. Quantify empty/full capsid content of viral vectors like AAVs.

SARS-CoV-2

SARS-CoV-2 Research

 

Reduce your lab time while accelerating your COVID‑19 research. Set up, leave and remotely review your data in just 3 hours!

SARS-CoV-2 Serology

SARS-CoV-2 Serology Assay

 

Rapid quantitative characterization of human IgG antibodies in serum or plasma reactive against multiple SARS-CoV-2 antigens.