Jess Automated Western Blot System

"Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."

"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."

"Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease"

"The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy."

"It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months."

"One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods."

"We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection."

"The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies."

"We had been trying to study receptor proteins with traditional Westerns but it was frustrating—we just could not get it. A few months with Wes and we've already had way more success than we had in three years."

"The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. What's even better is that [Simple Western] can run up to 40 proteins in three days. It would take about a week to run 40 proteins using Western blotting."

"The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-Iα and PKG-Iβ and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies."

"Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. And obviously I like getting results the same day rather than having to wait two days"

"The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. I really like this system over traditional Western."

"We’ve been able to increase productivity dramatically with Wes. The results are much better too, given that it removes the blotting step all together."

"[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage."

"Some of the proteins we wanted to detect are low in abundance and it’s really hard to get a good amount of protein for traditional Western blot. The low protein concentrations required by [Simple Western] makes it easier to save precious samples ensuring protein detection."

"Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal."

"[Wes] Provides quantitative data, saves time and uses far less sample."

"I was able to screen various antibodies in a single run within half a day. This had previously taken weeks and months using traditional Western blotting methods"

"I can run immunoassays on Wes for an entire animal experiment in a single day. This is something I wouldn’t even try with traditional Western blots."

"Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps."

"It’s had a significant impact on our research... we get double the amount of data in a third of the time."

"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."

"The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results."

"Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. Jess’s ability to detect multiple proteins per sample further saves time compared to conventional Western blot."

"It saves so much time! It takes away so much of the pipetting and manipulation error. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities."

"By switching my assays over to Simple Western, I am able to increase my throughput, allowing for full dose responses to be generated in each experiment. Utilizing the RePlex module even further increases this throughput as I often probe for 5-6 proteins per capillary in a single run including phosphorylated/total protein pairs in the same lane."

"I used Simple Western because the protein samples I had were small. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate."

"Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time."

"Jess is a user-friendly, multi-purpose platform that gives me the possibility to perform Western blot analysis on precious samples, as well as quantifying proteins expressed in the cells at the same time."

"Our lab came across ProteinSimple’s Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables."