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Profile heterogeneity in your samples by measuring protein expression in thousands of single cells in a single run. All you need to do is load your cell suspension. The scWest chip captures ~1,000 single-cells. Once everything is ready, Milo performs a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Finally, probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies.

Unlock the single-cell proteome

Left Side of Milo Single-cell Simple Western Blot Instrument by ProteinSimple

Milo

The world's first Single-Cell Western platform

Requirements & compatibility

Sample type

Suspension containing >10,000 cells

Cell diameter

7-25 µm in suspension

Cell type

Mammalian cells; globular in suspension and unfixed

Antibody requirement

Standard unlabeled primaries and fluorescent secondaries

Other equipment needed

Open-format fluorescence microarray scanner capable of 5 µm resolution

Performance & specifications

Typical cell dilutions

Yield capture and analysis of 1,000-2,000 cells per scWest chip

Molecular weight (MW) range

15-175 kDa

MW resolution

10% difference in distinct spectral channels, as low as 30% differences in same spectral channel

Typical target multiplexing

Up to four proteins per cell by spectral and size-based multiplexing
Twelve-plus proteins per cell using stripping & reprobing

Workflow time

4-6 hours