Single-Cell Western

Unlock the Single-Cell Proteome with Milo

MILO Single-Cell Western Platform Hero Image

Meet Milo

Milo™ performs Single-Cell Western blot analysis on 1,000 single cells in one 4-hour experiment.

Milo Consumables

The one-stop-shop for all the consumables you need to perform Single-Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.

Milo Consumables

Milo Antibodies

Milo is an open platform so you can use any antibody to probe your target. Find antibodies validated for Milo.

Milo Antibody Database

The Single-Cell Western Has Arrived

Milo is the world’s first Single-Cell Western blotting platform that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies. Simply load your cell suspension on a scWest chip, then Milo performs lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Probe the scWest chip with conventional Western blot antibodies and use a microarray scanner to measure ~12 proteins per cell using a variety of multiplexing strategies, or store your chip for up to 9 months for future analysis.

Request Single-Cell Western Pricing

Bioprocessing Cell Heterogeneity Protein Expression with Milo

Discover the World of Single-Cell Proteomics with Milo

Here are just a few examples of the single-cell analysis studies that are made possible with Single-Cell Western blot analysis on Milo.

HIF1-a analysis with single cell western - Milo

Profile Protein and Cell-Type Heterogeneity

Profile the target protein expression heterogeneity and cell-type heterogeneity in your samples with Milo’s single-cell analysis that would go undetected by traditional Western blot.

Multiplexed Analysis of Single Red Blood Cells

Study Cell Heterogeneity in the Tumor Microenvironment

6-well culture dish with different immune cell types in each well

Monitor the Differentiation of Stem Cells

Check out these App Notes to learn how Single-Cell Western blotting with Milo can track and quantify stem cell differentiation into neural cell subtypes and cardiomyocytes:

Identify and Quantify Neural Cell Subtypes

Track iPSC to Cardiomyocyte Differentiation

Easier than Flow Cytometry

Milo is easier to use than other single-cell analysis technologies like flow cytometry. With Milo’s single-cell analysis, you can detect proteins in single cells that escape detection by flow cytometry due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single-cell analysis on Milo.

Learn How Milo Streamlines Your Flow Cytometry Experiments

Single-cell Western Cell and Gene Therapy - sickle cell analysis

Develop New Cell and Gene Therapies

Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new cell and gene therapies.

Track Gene Editing Efficiency

Milo protein validation workflow of single-cell RNA seq data

Validate Single-Cell Sequencing Data

Pair Milo’s single-cell proteomics with single-cell sequencing workflows for complete single-cell multiomic analysis. Milo provides single-cell protein expression information to validate your single-cell RNA data.

Validate Single-Cell RNA-Seq

Publish Your Single-Cell Westerns in Premier Journals

Milo has proven its ability to provide new and profound scientific insight that traditional Western blots cannot. This fact is reflected in the rank of the journals that feature articles with Single-Cell Western data with the vast majority (74%) of articles featuring Single-Cell Western data in top journals with impact factors of 7 or greater including Science, Cell, Nature Methods, Nature Communications, and Cell Reports. 32% of Single-Cell Western publications are in journals considered here to be in the premier category with an impact factor greater than 21.

Learn How Single-Cell Western is a Proven, High-Impact Technology

Protein Simple let's you meet scientific standards by delivering publication-ready results

Additional Single-Cell Western Resources

Namocell instruments can be applied to study single cell genomics

Customer Success Stories

Chia-Wei Cheng, Ph.D.
Postdoctoral Fellow, MIT

With Milo, we observed a level of single-cell protein expression heterogeneity in our purified intestinal stem cell population that was not previously possible with conventional Western blotting.

M. Maya Kaelberer, Ph.D.
Postdoctoral Associate, Duke University School of Medicine

We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell.

Yi Zhong, M.D., Ph.D.
Research Associate, Memorial Sloan Kettering Cancer Center

Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population.

Prashant Vijay Thakkar, Ph.D.
Postdoctoral associate, Weill Cornell Medicine

I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies.

Sample type	Suspension containing >10,000 cells
Cell diameter	7-25 µm in suspension
Cell type	Mammalian cells; globular in suspension and unfixed
Antibody requirement	Standard unlabeled primaries and fluorescent secondaries
Other equipment needed	Open-format fluorescence microarray scanner capable of 5 µm resolution

Performance and Specifications

Typical cell dilutions	Yield capture and analysis of 1,000-2,000 cells per scWest chip
Molecular weight (MW) range	15-175 kDa
MW resolution	10% difference in distinct spectral channels, as low as 30% differences in same spectral channel
Typical target multiplexing	Up to four proteins per cell by spectral and size-based multiplexing Twelve-plus proteins per cell using stripping & reprobing
Workflow time	4-6 hours