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Unlock the Single-Cell Proteome with Milo from ProteinSimple, a Bio-Techne Brand

Milo instrument by ProteinSimple a Bio-Techne brand

Meet Milo

Milo™ performs Single-Cell Western blot analysis on 1,000 single cells in one 4-hour experiment. Analyze cell and protein heterogeneity, validate RNAseq data, streamline flow cytometry studies, monitor stem cell differentiation, and more. 

Single Cell Western consumables by ProteinSimple a Bio-Techne brand

Milo Consumables

The one-stop-shop for all the consumables you need to perform Single-Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.

Custom GMP Antibody graphic

Milo Antibodies

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. 

The Single-Cell Western Has Arrived

Milo is the world’s first Single-Cell Western blotting platform from ProteinSimple, a Bio-Techne brand, that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies. Simply load your cell suspension on a scWest chip, then Milo performs fast lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Then, you can probe the scWest chip with your favorite conventional Western blot antibodies and use a microarray scanner to measure ~12 proteins per cell using a variety of multiplexing strategies, or you can store your chip for at least 9 months for future single-cell analysis. 

Bioprocessing Cell Heterogeneity Protein Expression with Milo

Discover the World of Single-Cell Proteomics with Milo

Here are just a few examples of the single-cell analysis studies that are made possible with Single-Cell Western blot analysis on Milo.

HIF1-a analysis with single cell western - Milo

Profile Protein and Cell-Type Heterogeneity

6-well culture dish with different immune cell types in each well

Monitor the Differentiation of Stem Cells

Check out these App Notes to learn how Single-Cell Western blotting with Milo can track and quantify stem cell differentiation into neural cell subtypes and cardiomyocytes:

Single-cell Western Cell  and Gene Therapy - sickle cell analysis

Develop New Cell and Gene Therapies

Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new cell and gene therapies.

Milo protein validation workflow of single-cell RNA seq data

Validate Single-Cell Sequencing Data

Pair Milo’s single-cell proteomics with single-cell sequencing workflows for complete single-cell multiomic analysis. Milo provides single-cell protein expression information to validate your single-cell RNA data. Since mRNA levels do not always correlate with functional protein levels, pairing single-cell RNA data with single-cell protein expression data is critical to making accurate and complete conclusions about cellular function.

Milo data plots

Easier than Flow Cytometry

Milo is easier to use than other single-cell analysis technologies like flow cytometry. With Milo’s single-cell analysis, you can detect proteins in single cells that escape detection by flow cytometry due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single-cell analysis on Milo.

Publish Your Single-Cell Westerns in Premier Journals

Milo has proven its ability to pro­vide new and profound scientific insight that traditional Western blots cannot. This fact is reflected in the rank of the journals that feature articles with Single-Cell Western data with the vast majority (74%) of articles featuring Single-Cell Western data in top journals with impact factors of 7 or greater including Science, Cell, Nature Methods, Nature Communications and Cell Reports. In fact, 32% of Single-Cell Western publications are in journals considered here to be in the premier category with an impact factor greater than 21.

Protein Simple let's you meet scientific standards by delivering publication-ready results

Customer Success Stories

Requirements & compatibility

Sample type

Suspension containing >10,000 cells

Cell diameter

7-25 µm in suspension

Cell type

Mammalian cells; globular in suspension and unfixed

Antibody requirement

Standard unlabeled primaries and fluorescent secondaries

Other equipment needed

Open-format fluorescence microarray scanner capable of 5 µm resolution

Performance & specifications

Typical cell dilutions

Yield capture and analysis of 1,000-2,000 cells per scWest chip

Molecular weight (MW) range

15-175 kDa

MW resolution

10% difference in distinct spectral channels, as low as 30% differences in same spectral channel

Typical target multiplexing

Up to four proteins per cell by spectral and size-based multiplexing
Twelve-plus proteins per cell using stripping & reprobing

Workflow time

4-6 hours

Single-Cell Western Applications

Cancer & Immuno-Oncology

Cancer & Immuno-Oncology

Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.

Cell & Gene Therapy

Cell & Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Neuroscience

Neuroscience

Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.

Solutions for Cell Signaling and Signal Pathways Analysis

Cell Signaling

Analyze an entire signaling pathway overnight with quantitative multiplex detection of phospho/total isoforms and total protein normalization.