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Profile heterogeneity in your samples by measuring protein expression in thousands of single cells in a single run. All you need to do is load your cell suspension. The scWest chip captures ~1,000 single-cells. Once everything is ready, Milo performs a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Finally, probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies.
Unlock the Single-Cell Proteome
Single-Cell Western Consumables
Single-Cell Western Consumables
Consumables include probing chambers & scWestern Kits
Requirements & compatibility
|
Sample type |
Suspension containing >10,000 cells |
|---|---|
|
Cell diameter |
7-25 µm in suspension |
|
Cell type |
Mammalian cells; globular in suspension and unfixed |
|
Antibody requirement |
Standard unlabeled primaries and fluorescent secondaries |
|
Other equipment needed |
Open-format fluorescence microarray scanner capable of 5 µm resolution |
Performance & specifications
|
Typical cell dilutions |
Yield capture and analysis of 1,000-2,000 cells per scWest chip |
|---|---|
|
Molecular weight (MW) range |
15-175 kDa |
|
MW resolution |
10% difference in distinct spectral channels, as low as 30% differences in same spectral channel |
|
Typical target multiplexing |
Up to four proteins per cell by spectral and size-based multiplexing Twelve-plus proteins per cell using stripping & reprobing |
|
Workflow time |
4-6 hours |
Single-Cell Western Applications
Cancer & Immuno-Oncology
Cancer & Immuno-Oncology
Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
SARS-CoV-2
SARS-CoV-2
Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!
Cell & Gene Therapy
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Targeted Degradation
Targeted Degradation
Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.
SARS-CoV-2 Serology
SARS-CoV-2 Serology
Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!
Exosome
Exosome
A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.
