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Profile heterogeneity in your samples by measuring protein expression in thousands of single cells in a single run. All you need to do is load your cell suspension. The scWest chip captures ~1,000 single-cells. Once everything is ready, Milo performs a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Finally, probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies.
Requirements & compatibility
|
Sample type |
Suspension containing >10,000 cells |
|---|---|
|
Cell diameter |
7-25 µm in suspension |
|
Cell type |
Mammalian cells; globular in suspension and unfixed |
|
Antibody requirement |
Standard unlabeled primaries and fluorescent secondaries |
|
Other equipment needed |
Open-format fluorescence microarray scanner capable of 5 µm resolution |
Performance & specifications
|
Typical cell dilutions |
Yield capture and analysis of 1,000-2,000 cells per scWest chip |
|---|---|
|
Molecular weight (MW) range |
15-175 kDa |
|
MW resolution |
10% difference in distinct spectral channels, as low as 30% differences in same spectral channel |
|
Typical target multiplexing |
Up to four proteins per cell by spectral and size-based multiplexing Twelve-plus proteins per cell using stripping & reprobing |
|
Workflow time |
4-6 hours |
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