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Unlock the Single-Cell Proteome with Milo from ProteinSimple, a Bio-Techne Brand
Meet Milo
Meet Milo
Milo™ performs Single-Cell Western blot analysis on 1,000 single cells in one 4-hour experiment. Analyze cell and protein heterogeneity, validate RNAseq data, streamline flow cytometry studies, monitor stem cell differentiation, and more.
Milo Consumables
Milo Consumables
The one-stop-shop for all the consumables you need to perform Single-Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.
Milo Antibodies
Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date.
The Single-Cell Western Has Arrived
Milo is the world’s first Single-Cell Western blotting platform from ProteinSimple, a Bio-Techne brand, that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies. Simply load your cell suspension on a scWest chip, then Milo performs fast lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Then, you can probe the scWest chip with your favorite conventional Western blot antibodies and use a microarray scanner to measure ~12 proteins per cell using a variety of multiplexing strategies, or you can store your chip for at least 9 months for future single-cell analysis.
Discover the World of Single-Cell Proteomics with Milo
Here are just a few examples of the single-cell analysis studies that are made possible with Single-Cell Western blot analysis on Milo.
Profile Protein and Cell-Type Heterogeneity
Profile Protein and Cell-Type Heterogeneity
Profile the target protein expression heterogeneity and cell-type heterogeneity in your samples with Milo’s single-cell analysis that would go undetected by traditional Western blot.
Monitor the Differentiation of Stem Cells
Check out these App Notes to learn how Single-Cell Western blotting with Milo can track and quantify stem cell differentiation into neural cell subtypes and cardiomyocytes:
Develop New Cell and Gene Therapies
Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new cell and gene therapies.
Validate Single-Cell Sequencing Data
Pair Milo’s single-cell proteomics with single-cell sequencing workflows for complete single-cell multiomic analysis. Milo provides single-cell protein expression information to validate your single-cell RNA data. Since mRNA levels do not always correlate with functional protein levels, pairing single-cell RNA data with single-cell protein expression data is critical to making accurate and complete conclusions about cellular function.
Easier than Flow Cytometry
Milo is easier to use than other single-cell analysis technologies like flow cytometry. With Milo’s single-cell analysis, you can detect proteins in single cells that escape detection by flow cytometry due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single-cell analysis on Milo.
Publish Your Single-Cell Westerns in Premier Journals
Milo has proven its ability to provide new and profound scientific insight that traditional Western blots cannot. This fact is reflected in the rank of the journals that feature articles with Single-Cell Western data with the vast majority (74%) of articles featuring Single-Cell Western data in top journals with impact factors of 7 or greater including Science, Cell, Nature Methods, Nature Communications and Cell Reports. In fact, 32% of Single-Cell Western publications are in journals considered here to be in the premier category with an impact factor greater than 21.
Customer Success Stories
Chia-Wei Cheng, Ph.D.
Postdoctoral Fellow, MIT
Postdoctoral Fellow, MIT
With Milo, we observed a level of single-cell protein expression heterogeneity in our purified intestinal stem cell population that was not previously possible with conventional Western blotting.
M. Maya Kaelberer, Ph.D.
Postdoctoral Associate, Duke University School of Medicine
Postdoctoral Associate, Duke University School of Medicine
We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell.
Yi Zhong, M.D., Ph.D.
Research Associate, Memorial Sloan Kettering Cancer Center
Research Associate, Memorial Sloan Kettering Cancer Center
Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population.
Prashant Vijay Thakkar, Ph.D.
Postdoctoral associate, Weill Cornell Medicine
Postdoctoral associate, Weill Cornell Medicine
I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies.
Requirements & compatibility
|
Sample type |
Suspension containing >10,000 cells |
|---|---|
|
Cell diameter |
7-25 µm in suspension |
|
Cell type |
Mammalian cells; globular in suspension and unfixed |
|
Antibody requirement |
Standard unlabeled primaries and fluorescent secondaries |
|
Other equipment needed |
Open-format fluorescence microarray scanner capable of 5 µm resolution |
Performance & specifications
|
Typical cell dilutions |
Yield capture and analysis of 1,000-2,000 cells per scWest chip |
|---|---|
|
Molecular weight (MW) range |
15-175 kDa |
|
MW resolution |
10% difference in distinct spectral channels, as low as 30% differences in same spectral channel |
|
Typical target multiplexing |
Up to four proteins per cell by spectral and size-based multiplexing Twelve-plus proteins per cell using stripping & reprobing |
|
Workflow time |
4-6 hours |
Single-Cell Western Applications
Cancer & Immuno-Oncology
Cancer & Immuno-Oncology
Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
Cell & Gene Therapy
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Neuroscience
Neuroscience
Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.
Cell Signaling
Analyze an entire signaling pathway overnight with quantitative multiplex detection of phospho/total isoforms and total protein normalization.
