Milo: Single-Cell Western Platform

Profile heterogeneity in your samples by measuring protein expression in thousands of single cells in a single run. All you need to do is load your cell suspension. The scWest chip captures ~1,000 single-cells. Once everything is ready, Milo performs a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Finally, probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies. ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple.

Unlock the Single-Cell Proteome

Milo

The world's first Single-Cell Western platform

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Single-Cell Western Consumables

Consumables include probing chambers & scWestern Kits

Order Single-Cell Western Consumables

Requirements & compatibility

Sample type	Suspension containing >10,000 cells
Cell diameter	7-25 µm in suspension
Cell type	Mammalian cells; globular in suspension and unfixed
Antibody requirement	Standard unlabeled primaries and fluorescent secondaries
Other equipment needed	Open-format fluorescence microarray scanner capable of 5 µm resolution

Performance & specifications

Typical cell dilutions	Yield capture and analysis of 1,000-2,000 cells per scWest chip
Molecular weight (MW) range	15-175 kDa
MW resolution	10% difference in distinct spectral channels, as low as 30% differences in same spectral channel
Typical target multiplexing	Up to four proteins per cell by spectral and size-based multiplexing Twelve-plus proteins per cell using stripping & reprobing
Workflow time	4-6 hours