Milo
Single-Cell Westerns
Single-Cell your Western with Milo
Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. Identify target expression heterogeneity, visualize cell subpopulations you can’t see with conventional western blots, simplify phospho-flow studies, and validate your single-cell RNA-seq data at the protein level.Citations for Milo
View all citations for the Single-Cell Western platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
More Information About Milo
Milo Applications
Cancer & Immuno-Oncology
Cancer & Immuno-Oncology
Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
SARS-CoV-2
SARS-CoV-2
Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!
Cell & Gene Therapy
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Targeted Degradation
Targeted Degradation
Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.
SARS-CoV-2 Serology
SARS-CoV-2 Serology
Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!
Exosome
Exosome
A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.
Feature | Description |
---|---|
Requirements & Compatibility | |
Sample type | Suspension containing >10,000 cells |
Cell diameter | 7-25 µm in suspension |
Cell type | Mammalian cells; globular in suspension and unfixed |
Antibody requirement | Standard unlabeled primaries and fluorescent secondaries |
Other equipment needed | Open-format fluorescence microarray scanner capable of 5 µm resolution |
Performance & Specifications | |
Typical cell dilutions | Yield capture and analysis of 1,000-2,000 cells per scWest chip |
Molecular weight (MW) range | 15-175 kDa |
MW resolution | 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel |
Typical target multiplexing | Up to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing |
Workflow time | 4-6 hours |