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Milo System - Single-Cell Westerns
I need to profile heterogeneity in complex samples
I need multiplexing to detect multiple proteins at the single-cell level
I need to simplify my phospho-flow signaling studies
I need to detect proteins that lack good flow antibodies
I want to measure protein isoform heterogeneity

Single-Cell Westerns

Milo™ is the world's first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.

Single-Cell your Western with Milo

Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. Identify target expression heterogeneity, visualize cell subpopulations you can’t see with conventional western blots, simplify phospho-flow studies, and validate your single-cell RNA-seq data at the protein level.

More Information About Milo

Requirements & Compatibility
Sample type Suspension containing >10,000 cells
Cell diameter 7-25 µm in suspension
Cell type Mammalian cells; globular in suspension and unfixed
Antibody requirement Standard unlabeled primaries and fluorescent secondaries
Other equipment needed Open-format fluorescence microarray scanner capable of 5 µm resolution
Performance & Specifications
Typical cell dilutions Yield capture and analysis of 1,000-2,000 cells per scWest chip
Molecular weight (MW) range 15-175 kDa
MW resolution 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel
Typical target multiplexing Up to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing
Workflow time 4-6 hours