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Milo System - Single-Cell Westerns
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I need to profile heterogeneity in complex samples
I need multiplexing to detect multiple proteins at the single-cell level
I need to simplify my phospho-flow signaling studies
I need to detect proteins that lack good flow antibodies
I want to measure protein isoform heterogeneity

Single-Cell Westerns

Milo™ is the world's first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.

Single-Cell your Western with Milo

Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. Identify target expression heterogeneity, visualize cell subpopulations you can’t see with conventional western blots, simplify phospho-flow studies, and validate your single-cell RNA-seq data at the protein level.

Citations for Milo

View all citations for the Single-Cell Western platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Testimonials from your Peers for Single-Cell Western

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"I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies."

Prashant Vijay Thakkar, Ph.D., Postdoctoral associate, Department of Medicine, Weill Cornell Medicine

"We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell."

M. Maya Kaelberer, Ph.D., Postdoctoral Associate, Duke University School of Medicine

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More Information About Milo

Milo Applications

Cancer & Immuno-Oncology

Cancer & Immuno-Oncology

Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.

SARS-CoV-2

SARS-CoV-2

Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!

Cell & Gene Therapy

Cell & Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
 

Targeted Degradation

Targeted Degradation

Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

SARS-CoV-2 Serology

SARS-CoV-2 Serology

Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!

Exosome

Exosome

A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.

Feature Description
Requirements & Compatibility
Sample type Suspension containing >10,000 cells
Cell diameter 7-25 µm in suspension
Cell type Mammalian cells; globular in suspension and unfixed
Antibody requirement Standard unlabeled primaries and fluorescent secondaries
Other equipment needed Open-format fluorescence microarray scanner capable of 5 µm resolution
Performance & Specifications
Typical cell dilutions Yield capture and analysis of 1,000-2,000 cells per scWest chip
Molecular weight (MW) range 15-175 kDa
MW resolution 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel
Typical target multiplexing Up to four proteins per cell by spectral and size-based multiplexing Twelve - plus proteins per cell using stripping & reprobing
Workflow time 4-6 hours

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