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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

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Single-Cell Western Validated Antibodies

Filter Antibodies: (395)
Validated Target Host Species Vendor Cat # Sample Concentration
α-actinin Goat R&D Systems AF8279 KPC cells 100 µg/mL
β-tubulin* Rabbit Novus Biologicals NB600-936 Mouse dendrites 1:40
APRIL Goat R&D Systems AF884 MOLT-4 cells 1:5
Atg7 Mouse R&D Systems MAB6608 HeLa cells 100 µg/mL
ATG7 Mouse R&D Systems MAB6608 Macrophages 1:10
ATM Mouse Novus Biologicals NB100-309 (unpublished) 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals NB300-540 Live primary human cortical neurons 100 µg/mL
BAFF Goat R&D Systems AF124 MOLT-4 cells 1:10
beta-tubulin Mouse Novus Biologicals NBP2-50061 Jurkat cells 50 µg/mL
beta-tubulin Mouse Novus Biologicals NB120-7792 Jurkat cells 50 µg/mL
betaIII-tubulin Mouse R&D Systems MAB1195 neural differentiated iPSCs 100 µg/mL
Calnexin Rabbit Novus Biologicals NBP1-97476 U373 MG cells 1:10
CCL3/MIP1 Goat R&D Systems AF-270-NA Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CCR7 Rabbit Novus Biologicals NBP2-58753 Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems AF140 Dendritic cells 100 µg/mL
CD11b Rabbit Novus Biologicals NB110-89474 (unpublished) 1:10
CD209 (DC-SIGN) Sheep R&D Systems AF161 Dendritic cells 100 µg/mL
CD25 Goat R&D Systems AF-223-NA Tregs 100 µg/mL
CD29 Goat R&D Systems AF2405 KPC cells 100 µg/mL
CD4 Goat R&D Systems AF-379-NA PBMCs 1:10
CD56 (NCAM-1) Goat R&D Systems AF2408 Differentiated NK cells 100 µg/mL
CD68 Rat Novus Biologicals NBP2-33337 RAW264.7 cells 100 µg/mL
CD68/SR-D1 Rat Novus Biologicals NBP2-80656 RAW264.7 cells 100 µg/mL
CD81 Mouse Novus Biologicals NBP2-53318 Gli36 cells 100 µg/mL
CD81* Mouse Novus Biologicals NBP1-44861 Gli36 cells 100 µg/mL
CD83 Goat R&D Systems AF2044 Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems AF-141-NA Dendritic cells 100 µg/mL
CRBN Rabbit Novus Biologicals NBP1-91810 HeLa cells 100 µg/mL
CRMP5 Rat Novus Biologicals NB100-74395 1:10
Cyclin D1 Rabbit R&D Systems AF4196 MCF-7 cells 100 µg/mL
Cyclophilin B Goat R&D Systems AF5410 HEK293T cells 100 µg/mL
Cytochrome b reductase 1 Rabbit Novus Biologicals NBP2-16075 Red blood cells 100 µg/mL
DLL4 Goat R&D Systems AF1389 KPC cells 100 µg/mL
DNMT3A Goat R&D Systems AF6315 SH-SY5Y 20 µg/mL
E-cadherin Goat R&D Systems AF748 KPC cells 100 µg/mL
EGFR* Goat R&D Systems AF231 BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems AF960 (unpublished) 100 µg/mL
EphB4 Goat R&D Systems AF446 Neural stem cells 1:20
FAK Sheep R&D Systems AF4467 KPC cells 100 µg/mL
FLAG Tag Rabbit R&D Systems MAB8529 3T3 Flag-ATF6 cells 100 µg/mL
FOXP3 Goat R&D Systems BAF3240 Mouse T-regs
FOXP3 Rabbit Novus Biologicals NB100-39002 FoxP3-transfected HEK293T 100 µg/mL
GAPDH Mouse Novus Biologicals NB300-221 freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein 1:40
GAPDH Goat R&D Systems AF5718 HT-29 cells 100 µg/mL
GAPDH Goat Novus Biologicals NB300-320 Jurkat cells
GATA-3 Mouse R&D Systems MAB6330 MCF-7 cells 100 µg/mL
GATA-3 Mouse R&D Systems MAB26051 MCF-7 cells 100 µg/mL
GATA-3* Goat R&D Systems AF2605 MCF-7 cells 100 µg/mL
GDNF Goat R&D Systems AF-212-NA U-87 MG 20 µg/mL
GFAP Sheep R&D Systems AF2594 Human iPSCs 1:20

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