Skip to main content

Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species Vendor / Cat # Sample Concentration
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
β-tubulin* Rabbit Novus Biologicals
NB600-936
Mouse dendrites 1:40
APRIL Goat R&D Systems
AF884
MOLT-4 cells 1:5
Atg7 Mouse R&D Systems
MAB6608
HeLa cells 100 µg/mL
ATG7 Mouse R&D Systems
MAB6608
Macrophages 1:10
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals
NB300-540
Live primary human cortical neurons 100 µg/mL
BAFF Goat R&D Systems
AF124
MOLT-4 cells 1:10
beta-tubulin Mouse Novus Biologicals
NBP2-50061
Jurkat cells 50 µg/mL
beta-tubulin Mouse Novus Biologicals
NB120-7792
Jurkat cells 50 µg/mL
betaIII-tubulin Mouse R&D Systems
MAB1195
neural differentiated iPSCs 100 µg/mL
Calnexin Rabbit Novus Biologicals
NBP1-97476
U373 MG cells 1:10
CCL3/MIP1 Goat R&D Systems
AF-270-NA
Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CCR7 Rabbit Novus Biologicals
NBP2-58753
Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD11b Rabbit Novus Biologicals
NB110-89474
(unpublished) 1:10
CD209 (DC-SIGN) Sheep R&D Systems
AF161
Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA
Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405
KPC cells 100 µg/mL
CD4 Goat R&D Systems
AF-379-NA
PBMCs 1:10
CD56 (NCAM-1) Goat R&D Systems
AF2408
Differentiated NK cells 100 µg/mL
CD68 Rat Novus Biologicals
NBP2-33337
RAW264.7 cells 100 µg/mL
CD68/SR-D1 Rat Novus Biologicals
NBP2-80656
RAW264.7 cells 100 µg/mL
CD81 Mouse Novus Biologicals
NBP2-53318
Gli36 cells 100 µg/mL
CD81* Mouse Novus Biologicals
NBP1-44861
Gli36 cells 100 µg/mL
CD83 Goat R&D Systems
AF2044
Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA
Dendritic cells 100 µg/mL
CRBN Rabbit Novus Biologicals
NBP1-91810
HeLa cells 100 µg/mL
CRMP5 Rat Novus Biologicals
NB100-74395
1:10
Cyclin D1 Rabbit R&D Systems
AF4196
MCF-7 cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410
HEK293T cells 100 µg/mL
Cytochrome b reductase 1 Rabbit Novus Biologicals
NBP2-16075
Red blood cells 100 µg/mL
DLL4 Goat R&D Systems
AF1389
KPC cells 100 µg/mL
DNMT3A Goat R&D Systems
AF6315
SH-SY5Y 20 µg/mL
E-cadherin Goat R&D Systems
AF748
KPC cells 100 µg/mL
EGFR* Goat R&D Systems
AF231
BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems
AF960
(unpublished) 100 µg/mL
EphB4 Goat R&D Systems
AF446
Neural stem cells 1:20
FAK Sheep R&D Systems
AF4467
KPC cells 100 µg/mL
FLAG Tag Rabbit R&D Systems
MAB8529
3T3 Flag-ATF6 cells 100 µg/mL
FOXP3 Goat R&D Systems
BAF3240
Mouse T-regs
FOXP3 Rabbit Novus Biologicals
NB100-39002
FoxP3-transfected HEK293T 100 µg/mL
GAPDH Mouse Novus Biologicals
NB300-221
freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein 1:40
GAPDH Goat R&D Systems
AF5718
HT-29 cells 100 µg/mL
GAPDH Goat Novus Biologicals
NB300-320
Jurkat cells
GATA-3 Mouse R&D Systems
MAB6330
MCF-7 cells 100 µg/mL
GATA-3 Mouse R&D Systems
MAB26051
MCF-7 cells 100 µg/mL
GATA-3* Goat R&D Systems
AF2605
MCF-7 cells 100 µg/mL
GDNF Goat R&D Systems
AF-212-NA
U-87 MG 20 µg/mL
GFAP Sheep R&D Systems
AF2594
Human iPSCs 1:20

Results Per Page 50 100 200