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Neuron-specific beta‑III Tubulin Antibody Best Seller

Bio-Techne includes R&D Systems | Catalog # MAB1195

Key Product Details

Species Reactivity

Multi-Species

Applications

Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1

Product Summary for Neuron-specific beta‑III Tubulin Antibody

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta‑III Tubulin Antibody

Detection of Human and Mouse  beta-III Tubulin antibody by Western Blot.

Detection of Human and Mouse beta‑III Tubulin by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for beta-III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta‑III Tubulin antibody in Rat Cortical Neurons and GFAP antibody in Rat Astrocytes by Immunocytochemistry (ICC).

beta‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes.

beta-III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta‑III Tubulin antibody in Differentiated Human Neural Progenitor Cells by Immunocytochemistry (ICC).

beta‑III Tubulin in Differentiated Human Neural Progenitor Cells.

beta‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Neuron-specific beta‑III Tubulin Antibody

Application
Recommended Usage

Immunocytochemistry

0.5-25 µg/mL
Sample: Immersion fixed rat cortical stem cells, rat cortical neurons, SH‑SY5Y human neuroblastoma cells (Positive) and HEL 92.1.7 human erythroleukemic cell line (Negative)

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cells

Simple Western

10 µg/mL
Sample: Rat cortical neurons

Western Blot

0.2 µg/mL
Sample: Human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 21 reviews rated 4.7 using MAB1195 in the following applications:

Published Applications

Read 331 publications using MAB1195 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-III Tubulin

beta-III Tubulin, also known as tubulin beta-4, is regarded as a neuron-specific marker. The expression of beta-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for Neuron-specific beta‑III Tubulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Neuron-specific beta‑III Tubulin Antibody

For research use only

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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