Neuron-specific beta-III Tubulin Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM1195
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Product Specifications
Immunogen
Rat brain-derived microtubules
Specificity
Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Neuron-specific beta-III Tubulin Biotinylated Antibody
beta-III Tubulin in Rat Cortical Stem Cells.
beta-III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using Neuron-specific beta-III Tubulin Biotinylated Monoclonal Antibody (Catalog # BAM1195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Neuron-specific beta-III Tubulin Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed differentiated rat cortical stem cells
Sample: Immersion fixed differentiated rat cortical stem cells
Western Blot
1 µg/mL
Sample: beta-III Tubulin
Sample: beta-III Tubulin
Reviewed Applications
Read 1 review rated 5 using BAM1195 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-III Tubulin
Class III beta tubulin, also known as tubulin beta-4, is regarded as a neuron-specific marker. The expression of class III beta-tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium (1, 2).
References
- Caccamo, D. et al. (1989) Lab. Invest. 60:390.
- Alexander, J. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4685.
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Entrez Gene IDs
10381 (Human)
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Documents for Neuron-specific beta-III Tubulin Biotinylated Antibody
Product Specific Notices for Neuron-specific beta-III Tubulin Biotinylated Antibody
For research use only
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