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Neuron-specific beta-III Tubulin NorthernLights™ NL557-conjugated Antibody

Catalog # NL1195R | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Species Reactivity

Multi-Species

Applications

Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1

Product Summary

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

beta‑III Tubulin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

beta‑III Tubulin in Rat Cortical Stem Cells.

beta‑III Tubulin was detected in immersion fixed 7 day differentiated rat cortical stem cells using a 1:10 dilution of Neuron-specific beta‑III Tubulin (Clone TuJ‑1) NorthernLights™-557 Conjugated Monoclonal Antibody (Catalog # NL1195R) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence

Detection of Mouse beta-III Tubulin by Immunocytochemistry/Immunofluorescence

Assessment of the roles of macrophages in corneal development. a Co-staining of limbal blood vessels/corneal nerve fibers and macrophages in mice at postnatal day 7 (P7). The upper image shows the immunostaining of the limbal blood vessels and macrophages with anti-CD31 antibody and anti-CD64 antibody (scale bar, 10 μm). The lower image shows the immunostaining of the corneal nerve fibers and macrophages with anti-beta -III-tubulin antibody and anti-CD64 antibody (scale bar, 10 μm). b The gating strategies of analyzing corneal macrophages with flow cytometry. Corneal macrophages in mice at P7 were classified into C–C chemokine receptor type 2 negative (CCR2−) and positive (CCR2+) populations. The gating strategies were justified by the related isotype controls in Supplementary Fig. 6. c The expression of genes involved in cell division (left image) and neurogenesis and angiogenesis (right image) in the flow-cytometry-sorted corneal CCR2− and CCR2+ macrophages with qPCR (n = 3 independent experiments, ten mice per experiment in each group). d Changes in the corneal development of anti-CSF1R antibody-treated mice (n = 6 mice at each time point in each group). e Alteration of the expression of genes involved in cell division, neurogenesis, and angiogenesis in corneal tissues of anti-CSF1R antibody-treated mice at P28 (n = 4 independent experiments, six mice per experiment in each group). f Changes in the corneal development of BMS CCR2 22-treated mice (n = 6 mice at each time point in each group). The data are presented as mean ± SD. *P 

Applications

Application
Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: Immersion-fixed 7 day differentiated rat cortical stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NL1195R in the following applications:

Published Applications

Read 8 publications using NL1195R in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 10X solution of antibody in PBS containing sodium azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: beta-III Tubulin

beta-III Tubulin, also known as tubulin beta-4, is regarded as a neuron-specific marker. The expression of beta-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices

For research use only

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