iCE™ platforms perform imaged capillary isoelectric focusing (cIEF) and capillary electrophoresis sodium dodecyl sulfate (CE-SDS) for reproducible, quantitative analysis of identity, purity, and heterogeneity profiles for your therapeutic proteins. And they do it fast—we're talking results in 15 minutes or less per sample! You'll also get high resolution, full-on automation, and none of the hassles that come with other IEF techniques. Maurice platforms also automate size separations for you, so you'll get robust and quantitative analysis for your monoclonal antibodies too.

iCE Instruments for cIEF and CE-SDS

Maurice​ One-stop cIEF and CE-SDS​ Purity, identity, and heterogeneity analysis​

Maurice

One-stop cIEF and CE-SDS

Purity, identity and heterogeneity analysis

Maurice S.​ Ready-to-go CE-SDS or CE-SDS PLUS​ Simplifies size separation workflows​

Maurice S.

Ready-to-go CE-SDS or CE-SDS PLUS

Simplifies size separation workflows

cIEF and CE-SDS with iCE

Maurice C.

cIEF data in a snap

Identity and charge heterogeneity

iCE3​ Current standard for cIEF protein charge heterogeneity analysis​

iCE3

Current standard for cIEF protein charge heterogeneity analysis

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Which imaged cIEF/CE-SDS system is right for you?

* with Alcott Autosampler

System

Maurice

Maurice C.

Maurice S.

iCE3

cIEF Charge Application

Yes

Yes

No

Yes

CE-SDS Size Application

Yes

No

Yes

No

Absorbance Detection

Yes

Yes

Yes

Yes

Fluorescence Detection

Yes

Yes

No

No

Onboard Mixing for Sample Prep

Yes

Yes

No

Yes*

Description

cIEF Specification

CE-SDS Specification

Minimum Sample Volume

50 µL

50 µL

Sample Delivery

Vacuum

Electrokinetic

Typical Separation Time

6-10 min (molecule-dependent)

UV Absorbance at 220 nm

Detection Capability

Pre-focusing: 1500 V, focusing: 3000 V

Separation: 5750 V

Sample Injections per Cartridge

100 guaranteed, 200 maximum

100 guaranteed, 200 maximum for CE-SDS, 500 maximum for CE-SDS PLUS

Maximum Sample Injections per Batch

100

48

pI/Size Range

2.85-10.45

10-270 kDa

pI/Sizing CV

1%

≤2%

CV for Peaks >10% Composition

≤5% (Intra-batch), ≤6% (Inter-batch)

N/A

pI/Sizing Resolution

2 logs

2 logs

Dynamic Range

2 logs

2 logs

Linearity

>0.995

>0.995

Sensitivity (LOD)

0.7 µg/mL (Native fluorescence)
3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody)

0.3 µg/mL
(Value based on internal standard)

Sample Tray Options

96-well plates or 48 vials

96-well plates or 48 vials

Power

100 V-240 V (AC), 50/60 Hz, 500 W

100 V-240 V (AC), 50/60 Hz, 500 W

Temperature Control Range

4-25 °C

4-25 °C

Dimensions

44cm H x 42cm W x 61cm D

44cm H x 42cm W x 61cm D

Weight

46 kg (100 lb)

46 kg (100 lb)