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iCE™ platforms perform imaged capillary isoelectric focusing (cIEF) and capillary electrophoresis sodium dodecyl sulfate (CE-SDS) for reproducible, quantitative analysis of identity, purity, and heterogeneity profiles for your therapeutic proteins. And they do it fast—we're talking results in 15 minutes or less per sample! You'll also get high resolution, full-on automation, and none of the hassles that come with other IEF techniques. Maurice platforms also automate size separations for you, so you'll get robust and quantitative analysis for your monoclonal antibodies too.
ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple.
iCE Instruments for cIEF and CE-SDS
Which imaged cIEF/CE-SDS system is right for you?
|
System |
Maurice |
Maurice C. |
Maurice S. |
iCE3 |
|---|---|---|---|---|
|
cIEF Charge Application |
Yes |
Yes |
No |
Yes |
|
CE-SDS Size Application |
Yes |
No |
Yes |
No |
|
Absorbance Detection |
Yes |
Yes |
Yes |
Yes |
|
Fluorescence Detection |
Yes |
Yes |
No |
No |
|
Onboard Mixing for Sample Prep |
Yes |
Yes |
No |
Yes* |
|
Description |
cIEF Specification |
CE-SDS Specification |
|---|---|---|
|
Minimum Sample Volume |
50 µL |
50 µL |
|
Sample Delivery |
Vacuum |
Electrokinetic |
|
Typical Separation Time |
6-10 min (molecule-dependent) |
UV Absorbance at 220 nm |
|
Detection Capability |
Pre-focusing: 1500 V, focusing: 3000 V |
Separation: 5750 V |
|
Sample Injections per Cartridge |
100 guaranteed, 200 maximum |
100 guaranteed, 200 maximum for CE-SDS, 500 maximum for CE-SDS PLUS |
|
Maximum Sample Injections per Batch |
100 |
48 |
|
pI/Size Range |
2.85-10.45 |
10-270 kDa |
|
pI/Sizing CV |
1% |
≤2% |
|
CV for Peaks >10% Composition |
≤5% (Intra-batch), ≤6% (Inter-batch) |
N/A |
|
pI/Sizing Resolution |
2 logs |
2 logs |
|
Dynamic Range |
2 logs |
2 logs |
|
Linearity |
>0.995 |
>0.995 |
|
Sensitivity (LOD) |
0.7 µg/mL (Native fluorescence) 3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody) |
0.3 µg/mL (Value based on internal standard) |
|
Sample Tray Options |
96-well plates or 48 vials |
96-well plates or 48 vials |
|
Power |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
|
Temperature Control Range |
4-25 °C |
4-25 °C |
|
Dimensions |
44cm H x 42cm W x 61cm D |
44cm H x 42cm W x 61cm D |
|
Weight |
46 kg (100 lb) |
46 kg (100 lb) |
iCE Applications
Bioprocessing
Whether it is during clone selection or final product characterization, use a hassle-free, robust, and fast method to characterize the charge and size profiles of your therapeutic.
CE-SDS
Get automated, reproducible, and high-throughput CE-SDS analysis in as little as 25 minutes per sample.
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Protein Charge Heterogeneity
Protein Charge Heterogeneity
High-quality absorbance and native fluorescence give you charge heterogeneity data for even the most complex proteins with automated, whole-column cIEF.
Protein Stability and Formulation Testing
Protein Stability and Formulation Testing
Understand the impact of protein changes on drug safety and efficacy and develop the best formulation for your therapeutic with cIEF analysis.
Protein & Antibody Characterization
Protein & Antibody Characterization
Rapidly and accurately monitor the charge and molecular weight heterogeneity of your proteins, two critical properties that inevitably change during development.
Post-translation Modification
Post-translation Modification
To know if your proteins have undergone deamidation, oxidation, glycation, or another other post-translational modification, get accurate data on their changing isoelectric points.
Biosimilars
Biosimilars
Gain confidence in your biosimilars by getting accurate, high-quality data on the charge and size profiles of your proteins and antibodies.
Vaccine Development
Vaccine Development
Whether you’re working with LNPs or viral vectors, confirm their charge heterogeneity, identity, and purity, and proceed confidently with your vaccine development.
