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Maurice
Your workhorse station for purity, identity and heterogeneity analysis
Purity, identity and heterogeneity
Still spending weeks or months on method development for your biologics? This new advanced CE platform lets you easily switch between cIEF and CE-SDS applications and develop platform methods within a day. In this short video, the people behind the gold-standard iCE technology highlight the key benefits of the new platform for CE analysts. Find out how Maurice makes CE easy with his streamlined workflow and delivers identity, purity and heterogeneity data on your biologics fast.|
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My molecule has complicated charge profiles that are difficult to resolveMaurice affords high-resolution characterization of charge variants by preserving our proprietary imaged capillary isoelectric focusing (icIEF) technology, which has been the go-to method for measuring charge heterogeneity. The whole-column detection eliminates the post-focusing mobilization step needed with traditional cIEF, and can resolve your most challenging molecules including fusion proteins and hydrophobic ADCs. |
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My current method is not reproducible and error-proneMaurice employs a prequalified, ready-to-use cartridge design which fully automates column conditioning and clean up. No laborious capillary assembly or maintenance is required. The on-board sample mixing feature further enhances the ease-of-use, and reduces operator-dependent variability commonly encountered with other systems. |
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I have many samples and my schedule is tightMaurice produces pI and charge heterogeneity data in less than 10 minutes, and size-based CE-SDS data in 35 minutes. The fast separation time, flexible workflow and high reproducibility makes Maurice a true lab workhorse. In one day you can complete your method development, or analyze 48 to 100 samples in one batch. The icing? You can develop platform methods and use them for multiple molecules too. |
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I am concerned about cross-contamination between applicationsMaurice employs completely separate fluid paths for cIEF and CE-SDS applications. Waste is collected inside the cartridge which removes the concern about cross-contamination between experiments. In addition, when dealing with molecules such as ADCs, no more hassle about handling the toxic waste. |
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My sample is limited and they tend to aggregate at high concentrationMaurice's native fluorescence detection for cIEF provides 3-5X higher sensitivity than UV absorption, which means you can now measure sample at concentration as low as 0.7 µg/ml. And because proteins tend to aggregate less at lower concentrations you can reduce or even eliminate urea completely in some of your methods. Baselines are also significantly cleaner and less sensitive to ampholyte interference, giving you more options when optimizing your pH gradient. |
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I work in a GMP lab and data integrity is key to my compliance requirementCompass for iCE, the software package Maurice uses to acquire, manage and analyze data, has all the tools required for 21 CFR compliance so you'll have no issue analyzing biotherapeutics on Maurice in a regulated environment. |
Citations for Maurice
View all citations for the Imaged Capillary Electrophoresis (iCE) platform.
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More Information About Maurice
| Feature | cIEF Specification | CE-SDS Specificiation |
|---|---|---|
| Minimum Sample Volume | 50 µL | 50 µL |
| Sample Delivery | Vacuum | Electrokinetic |
| Typical Separation Time | 6–10 min (molecule-dependent) | Reduced IgG: 25 min, Non-reduced IgG: 35 min |
| Detection Capability |
UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320– 450 nm |
UV Absorbance at 220 nm |
| Typical Voltage | Pre-focusing: 1500 V, focusing: 3000 V | Separation: 5750 V |
| Sample Injections per Cartridge | 100 guaranteed, 200 maximum | 100 guaranteed, 200 maximum for CE-SDS, 500 maximum for CE-SDS PLUS |
| Maximum Sample Injections per Batch | 100 | 48 |
| pI/Size Range | 2.85–10.45 | 10–270 kDa |
| pI/Sizing CV | 0.01 | ≤2% |
| CV for Peaks >10% Composition | ≤5% (Intra-batch), ≤6% (Inter-batch) | N/A |
| Relative Migration Time CV | N/A | <1% for reduced IgG |
| pI/Sizing Resolution | 0.05 pI units (for wide range 3–10 ampholytes) | ≥1.5 for NGHC/HC IgG Standard |
| Dynamic Range | 2 logs | 2 logs |
| Linearity | >0.995 | >0.995 |
| Sensitivity (LOD) | 0.7 µg/mL (Native fluorescence) 3.0 µg / mL(Absorbance) (Values based on a monoclonal antibody) | 0.3 µg/mL (Value based on Internal Standard) |
| Sample Tray Options | 96-well plates or 48 vials | |
| Dimensions | 44cm H x 42cm W x 61cm D | |
| Weight | 46 kg (100 lb) | |
