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Profiling Changes in Receptor Tyrosine Kinase Phosphorylation Using Antibody Arrays
Watch this video to learn about the principle of the Proteome Profiler antibody array. See how assays are run with this step-by-step detailed description of the method.
Understanding Immunosuppressive Myeloid Cells and Immunotherapy Using Proteome Profiler Array
Advanced Prostate Cancer (PCa) can progress to lethal metastatic castration-resistant prostate cancer (mCRPC), even when treated with androgen deprivation therapy. Although immune checkpoint blockade has revolutionized the way cancer is treated, it is less effective in this context. Watch this video to see how Proteome Profiler antibody arrays were used by Dr. Xin Lu, and his research team as they addressed this challenge. See how they demonstrated increased synergistic antitumor efficacy when an immune checkpoint blockade cocktail (CTLA-4 and PD1) is used in combination with PI3-kinase signaling pathway inhibitors. Find out about the role that myeloid-derived suppressor cells play in aggressive cancer.
See how Proteome Profiler has been used in prostate cancer research.
See How the Proteome Profiler is Used to Detect Multiple Targets Simultaneously
Simultaneous Detection of Multiple Proteases Following Treatment with IL-1α and Oncostatin M (OSM).
The Proteome Profiler Human Protease Antibody Array (Catalog # ARY021B) was used to simultaneously assess protease levels in human chondrosarcoma cell lysates. Cells were either untreated (Top) or treated (Bottom) with Recombinant Human IL-1α (Catalog # 200-LA) and Recombinant Human OSM (Catalog # 295-OM). Chemiluminescence was detected using x-ray film.
The Proteome ProfilerTM Antibody Array is a simple, high throughput, yet cost-effective tool for early-stage analyte profiling. Superior specificity, low background noise and no cross-reactivity means that you can count on this assay for clear and consistent data. Nitro-cellulose membranes are pre-spotted in duplicate with high quality capture antibodies for multiplexing. Proteome Profiler antibody arrays come ready to use with everything you need. Simply incubate the antibody array in your lysate or supernate, rinse, and detect analytes with HRP or IRDye® conjugated antibodies. Use standard chemiluminescence or LI-COR technology to visualize data. No new capital purchase is required, just standard immunoblotting equipment. Proteome Profiler Antibody Arrays have a robust signal, lasting longer than that of some competitors. Assays are available for human, mouse and rat targets. Choose from intracellular targets such as kinases and other signaling proteins or extracellular targets such as cytokines, chemokines, adipokines and more.