Rat Nestin Antibody
Catalog # AF2736 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Rat Nestin Antibody
Immunogen
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Specificity
Clonality
Host
Isotype
Scientific Data Images for Rat Nestin Antibody
Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.beta‑III Tubulin and Nestin in Rat Cortical Stem Cells.
beta-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Rat Nestin Antibody
Immunocytochemistry
Sample: Immersion fixed rat cortical stem cells and immersion fixed rat cortical neurons
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of rat embyo (E15)
Western Blot
Sample: Recombinant Rat Nestin
Reviewed Applications
Read 4 reviews rated 4.5 using AF2736 in the following applications:
Published Applications
Read 10 publications using AF2736 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
Product Documents for Rat Nestin Antibody
Product Specific Notices for Rat Nestin Antibody
For research use only
Citations for Rat Nestin Antibody (10)
Citations are publications that use Bio-Techne products. Selected citations for Rat Nestin Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: ICCBC Fong et al. (2022-11-01), The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state Cell Reports, 2022-141(5):111578.
PMID: 36323247 -
Species: Mouse
Sample Types: Whole Cells
Applications: IFL Chen et al. (2021-10-04), Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells The Journal of Biological Chemistry, 2021-1297(5):101275.
PMID: 34619150 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCK Xi et al. (2020-09-09), Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery Nat Commun, 2020-011(1):4504.
PMID: 32908131 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PS Cheng et al. (2019-01-28), Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors J. Neurosci., 2019-00(0).
PMID: 30692224 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-FrY Takamori et al. (2018-11-05), Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex IBRO Rep, 2018-15(0):99-109.
PMID: 30505974 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-FrTakamori Y et al. (2009-03-21), Nestin-positive microglia in adult rat cerebral cortex. Brain Res., 2009-01270(0):10-8.
PMID: 19306852 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PGregorian C et al. (2009-02-11), Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis. J. Neurosci., 2009-029(6):1874-86.
PMID: 19211894 -
Species: Mouse
Sample Types: Tissue Homogenates,Whole Tissue
Applications: IHC-P,Western BlotZoukhri D et al. (2008-06-27), Mechanisms of murine lacrimal gland repair after experimentally induced inflammation. Invest. Ophthalmol. Vis. Sci., 2008-049(10):4399-406.
PMID: 18586880 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotDe Bank PA et al. (2007-08-15), Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking. Biotechnol. Bioeng., 2007-097(6):1617-25.
PMID: 17304565 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-FrSubburaju S et al. (2007-04-05), Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary. Endocrinology, 2007-0148(7):3102-10.
PMID: 17412807
There are no citations that match your criteria.
Customer Reviews for Rat Nestin Antibody (4)
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Application: ImmunofluorescenceSample Tested: See PMID 23399867Species: RatVerified Customer | Posted 01/07/2015
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Application: ImmunofluorescenceSample Tested: See PMID 23996239Species: HumanVerified Customer | Posted 01/07/2015
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Application: ImmunofluorescenceSample Tested: transfected RN22 cellsSpecies: RatVerified Customer | Posted 12/18/2014
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Application: Western BlotSample Tested: RN22 cellsSpecies: RatVerified Customer | Posted 12/18/2014
There are no reviews that match your criteria.
Reconstitution Calculator
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