Rat Nestin Antibody

Catalog # AF2736 | R&D Systems, Inc. a Bio-Techne Brand
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AF2736
AF2736-SP
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Key Product Details

Species Reactivity

Rat

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Rat Nestin Antibody

Immunogen

E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749

Specificity

Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat Nestin Antibody

Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Nestin in Rat Cortical Stem Cells.

Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta-III Tubulin and Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

beta‑III Tubulin and Nestin in Rat Cortical Stem Cells.

beta-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Nestin in Rat Cortical Stem Cells.

Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Rat Nestin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat cortical stem cells and immersion fixed rat cortical neurons

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embyo (E15)

Western Blot

0.1 µg/mL
Sample: Recombinant Rat Nestin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using AF2736 in the following applications:

Published Applications

Read 10 publications using AF2736 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

References

  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

UniProt

EDM00749

Product Documents for Rat Nestin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat Nestin Antibody

For research use only

Citations for Rat Nestin Antibody (10)

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Customer Reviews for Rat Nestin Antibody (4)

4.5 out of 5
4 customer ratings
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  • Name: Anonymous -
    Application: Immunofluorescence
    Sample Tested: See PMID 23399867
    Species: Rat
    Verified Customer | Posted 01/07/2015
  • Name: Anonymous -
    Application: Immunofluorescence
    Sample Tested: See PMID 23996239
    Species: Human
    Verified Customer | Posted 01/07/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: transfected RN22 cells
    Species: Rat
    Verified Customer | Posted 12/18/2014
  • Name: cynthia morimoto
    Application: Western Blot
    Sample Tested: RN22 cells
    Species: Rat
    Verified Customer | Posted 12/18/2014

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