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Key Product Details

Species Reactivity

Goat

Applications

Immunocytochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Polyclonal Donkey IgG

Product Summary for Goat IgG NorthernLights™ NL557-conjugated Antibody

Immunogen

Goat IgG

Specificity

Goat IgG (H+L)

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG NorthernLights™ NL557-conjugated Antibody

Detection of Goat IgG Primary Antibody by Immunocytochemistry.

Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control). Detection of human EGFR by incubating cells with 5 ug/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Goat IgG NorthernLights™ NL557-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Use at a 1:200 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NL001 in the following applications:

Published Applications

Read 36 publications using NL001 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG NorthernLights™ NL557-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat IgG NorthernLights™ NL557-conjugated Antibody

For research use only

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