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Key Product Details

Species Reactivity

Goat

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Donkey IgG

Product Summary for Goat IgG (H+L) Fluorescein-conjugated Antibody

Immunogen

Goat IgG

Specificity

Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG (H+L) Fluorescein-conjugated Antibody

Detection of Goat IgG Primary Antibody by Flow Cytometry

Detection of Goat IgG Primary Antibody by Flow Cytometry

Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) at 10 uL/106cells.

Applications for Goat IgG (H+L) Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of goat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 7 publications using F0109 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG (H+L) Fluorescein-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat IgG (H+L) Fluorescein-conjugated Antibody

For research use only

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