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Key Product Details

Species Reactivity

Goat

Applications

Simple Western, Western Blot

Label

HRP

Antibody Source

Polyclonal Donkey IgG

Product Summary for Goat IgG HRP-conjugated Antibody

Immunogen

Goat IgG

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Goat IgG HRP-conjugated Antibody

Detection of Goat IgG Primary Antibody by Western Blot

Detection of Goat IgG Primary Antibody by Western Blot

Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9B Polyclonal Antibody (Catalog # AF3584) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Goat IgG Primary Antibody by Simple WesternTM.

Detection of Goat IgG Primary Antibody by Simple WesternTM.

Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog # AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Goat IgG HRP-conjugated Antibody

Application
Recommended Usage

Simple Western

Use at a 1:50 dilution.

Western Blot

Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4 using HAF109 in the following applications:

Published Applications

Read 124 publications using HAF109 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied as a 0.2 um filtered solution in PBS with stabilizers.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat IgG HRP-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Goat IgG HRP-conjugated Antibody

For research use only

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