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Goat IgG (H+L) APC‑conjugated Antibody
Catalog # F0108 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Goat
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Polyclonal Donkey IgG
Product Summary for Goat IgG (H+L) APC‑conjugated Antibody
Immunogen
Goat IgG
Specificity
Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Goat IgG (H+L) APC‑conjugated Antibody
Detection of Goat IgG Primary Antibody by Flow Cytometry
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL-P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3130, filled histogram) or isotype control antibody (Catalog # AB-108-C), open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) at 10 µL/106cells.Applications for Goat IgG (H+L) APC‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of goat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of goat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 14 publications using F0108 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG
Product Documents for Goat IgG (H+L) APC‑conjugated Antibody
Product Specific Notices for Goat IgG (H+L) APC‑conjugated Antibody
For research use only
Citations for Goat IgG (H+L) APC‑conjugated Antibody (14)
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Citations are publications that use Bio-Techne products. Selected citations for Goat IgG (H+L) APC‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySakemura et al. (2023-06-28), AXL inhibition improves the anti-tumor activity of chimeric antigen receptor T cells Cancer immunology research
PMID: 37378662 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKhan et al. (2023-05-30), Generation of a SARS-CoV-2 Reverse Genetics System and Novel Human Lung Cell Lines That Exhibit High Virus-Induced Cytopathology Viruses
PMID: 37376581 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryB Ahmad et al. (2022-08-09), The Effects of Hypoxic Preconditioned Murine Mesenchymal Stem Cells on Post-Infarct Arrhythmias in the Mouse Model International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012110 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryXF Hu et al. (2021-01-30), Impairment of type H vessels by NOX2-mediated endothelial oxidative stress: critical mechanisms and therapeutic targets for bone fragility in streptozotocin-induced type 1 diabetic mice Theranostics, 2021-011(8):3796-3812.
PMID: 33664862 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryQ Fan et al. (2020-12-09), ZIP4 Is a Novel Cancer Stem Cell Marker in High-Grade Serous Ovarian Cancer Cancers, 2020-112(12).
PMID: 33316986 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryX Pei et al. (2020-06-03), Development of AAV Variants with Human Hepatocyte Tropism and Neutralizing Antibody Escape Capacity Mol Ther Methods Clin Dev, 2020-018(0):259-268.
PMID: 32637455 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryW Shao et al. (2019-09-30), Superior human hepatocyte transduction with adeno-associated virus vector serotype 7 Gene Ther., 2019-00(0).
PMID: 31570819 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySA Felt et al. (2017-07-27), Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus J. Virol., 2017-00(0).
PMID: 28566376 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHuang X et al. (2015-07-14), IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas. PLoS ONE, 2015-010(7):e0133152.
PMID: 26173023 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTran et al. (2014-06-05), Oxidative stress decreases functional airway mannose binding lectin in COPD. PLoS ONE, 2014-09(6):e98571.
PMID: 24901869 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow CytometryRichardson et al. (2014-05-01), A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis, 2014-08(5):e2825.
PMID: 24786606 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKong F et al. (2013-02-18), Engineering a single ubiquitin ligase for the selective degradation of all activated ErbB receptor tyrosine kinases. Oncogene, 2013-033(8):986-95.
PMID: 23416973 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKinzfogl J et al. (2011-05-31), Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells. Blood, 2011-0118(3):565-75.
PMID: 21628410 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryHudecek M et al. (2010-08-11), The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor. Blood, 2010-0116(22):4532-41.
PMID: 20702778
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FAQs for Goat IgG (H+L) APC‑conjugated Antibody
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Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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