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Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody

Catalog # F0101B | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity



Flow Cytometry


Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody


Mouse IgG heavy and light chains


Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors







Scientific Data Images for Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody

Detection of Mouse IgG Primary Antibody by Flow Cytometry

Detection of Mouse IgG Primary Antibody by Flow Cytometry

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calreticulin-2 Monoclonal Antibody (Catalog # MAB2927, filled histogram) or isotype control antibody (Catalog # MAB0041), open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) at 10 µL/106cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody

Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using F0101B in the following applications:

Published Applications

Read 8 publications using F0101B in the following applications:

Formulation, Preparation, and Storage


Antigen Affinity-purified


Supplied in a saline solution containing BSA and Sodium Azide.


The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Product Documents for Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse F(ab)2 IgG (H+L) APC‑conjugated Antibody

For research use only

Setting the standard in quality research reagents