Mouse IgG2B Isotype Control

Name: Mouse IgG2B Isotype Control
Brand: R&D Systems
SKU: MAB0041

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB0041

Citations (8)

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Key Product Details

Immunogen:	KLH
Applications:	Control
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 133303
Specificity:	Detects KLH. Does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhALP, rhBLC/BCA-1, rmBLC, rmC10, rhCCL28, rmCCL28, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, rmC-TACK, recombinant viral (rv) CMV UL146, rvCMV UL147, rmCRG-2, rhCXCL16, rmCXCL16, rhCXC-X3, rmCXC-X3, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, recombinat cotton rat (rcr) IP-10, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rmLungkine, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rmMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rcrMIP-1 alpha, rhMIP-1 alpha, rmMIP-1 alpha, rcrMIP-1 beta, rhMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhPF4, rmPF4, rcrRANTES, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, rmTeck, or rmThymus CK1.

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Applications for Mouse IgG2B Isotype Control

	Recommended Concentration	Sample
Control	Negative control for flow cytometry experiments. Use at the same concentration as the detection antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IgG2B Isotype Control

	Control Detection of Mouse IgG Control by Flow Cytometry K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G2B Fc
Alternate Names	IgG2B

Citations for Mouse IgG2B Isotype Control (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IgG2B Isotype Control include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

A Stepanjuk et al. (2019), MUC20 expression marks the receptive phase of the human endometrium Reprod. Biomed. Online, 0(0).
PMID: 31519421
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype Control

Nielsen M et al. (2015), NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 135(5):1311-9.
PMID: 25634359
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control

Cerny D et al. (2014), Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection. PLoS Pathog, 10(12):e1004548.
PMID: 25474532
Species: Human
Sample Types: Whole Cells
Applications: Control

Andersson M et al. (2012), Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses. Innate Immun, 18(3):531-40.
PMID: 22058091
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Chauchereau A et al. (2011), Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer. Exp. Cell Res., 317(3):262-75.
PMID: 20974126
Species: Human
Sample Types: Whole Cells
Applications: Control

Tang KF et al. (2008), Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem. Biophys. Res. Commun., 370(4):578-83.
PMID: 18395517
Species: Human
Sample Types: Whole Cells
Applications: Control

Bolovan-Fritts CA et al. (2007), Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 111(1):175-82.
PMID: 17895402
Species: Human
Sample Types: Whole Cells
Applications: Control

Weindl G et al. (2007), Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. J. Clin. Invest., 117(12):3664-3672.
PMID: 17992260

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Showing 1 - 8 of 8 citations Showing All

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