Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity: | Mouse |
Immunogen: | Mouse IgG heavy and light chains |
Applications: | Flow Cytometry |
Label: | Phycoerythrin (Excitation=488 nm, Emission=565-605 nm) |
Source: | Polyclonal Goat IgG |
Specificity: |
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors |
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Citations for Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (12)
Citations are publications that use Bio-Techne products. Selected citations for Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody include:
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Species: Human
Sample Types: Serum
Applications: Luminex DevelopmentJC Cicciarell et al. (2017), Renal Transplant Patients Biopsied for Cause and Tested for C4d, DSA, and IgG Subclasses and C1q: Which Humoral Markers Improve Diagnosis and Outcomes? J Immunol Res, 2017(0):1652931.
PMID: 28182088 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryE Tamayo-Orb et al. (2017), Podocalyxin promotes proliferation and survival in mature B-cell non-Hodgkin lymphoma cells Oncotarget, 8(59):99722-99739.
PMID: 29245936 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryRHP Wilbers et al. (2017), Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain PLoS ONE, 12(10):e0186317.
PMID: 29016674 -
Species: Human
Sample Types: Whole Cells
Applications: Secondary Antibody(2016), IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 90(0):88-95.
PMID: 27863335 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometrySuzuki-Karasaki M et al. (2014), Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells. Int J Oncol, 44(2):616-28.
PMID: 24337174 -
Gaudineau B et al. (2012), Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion. J Cell Sci, 125(0):4475-86.
PMID: 22767506 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry ControlMacLeod S et al. (2012), HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice. Cancer Gene Ther, 19(12):888-98.
PMID: 23099884 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHedlund M et al. (2011), Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 6(2):e16899.
PMID: 21364924 -
Inagaki A et al. (2009), Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC. Int. J. Cancer, 125(1):212-21.
PMID: 19358282 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryTang KF et al. (2008), Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem. Biophys. Res. Commun., 370(4):578-83.
PMID: 18395517 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryOgbomo H et al. (2007), Histone deacetylase inhibitors suppress natural killer cell cytolytic activity. FEBS Lett., 581(7):1317-22.
PMID: 17349632 -
Species: Human
Sample Types: Whole Cells
Applications: Secondary AntibodyEbert EC et al. (2006), Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 241(1):7-13.
PMID: 16914126
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Customer Reviews for Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (3)
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Application: FlowSample Tested: Whole bloodSpecies: HumanVerified Customer | Posted 12/17/2020Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed twice with PBS and cells were aliquoted into centrifuge tubes for staining. Cells were counted and concentration was adjusted to 10,000 cells per uL. 100 uL of cells was aliquoted into each centrifuge tube for staining.
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Application: FlowSample Tested: Bone ExtractsSpecies: HumanVerified Customer | Posted 02/13/2020
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Application: FlowSample Tested: Breast cancer cellsSpecies: HumanVerified Customer | Posted 03/24/2017106 MA-11 cells were incubated with Mouse anti-Human CD44 antibody (red) or Isotype Control IgG (black), followed by staining with 10 uL of PE-conjugated Goat Anti-Mouse secondary antibody (#F0102B).
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