Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody Best Seller

Catalog # F0102B | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody

Immunogen

Mouse IgG heavy and light chains

Specificity

Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody

Detection of Mouse IgG Primary Antibody by Flow Cytometry

Detection of Mouse IgG Primary Antibody by Flow Cytometry

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD157 Monoclonal Antibody (Catalog # MAB47361, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) at 10 µL/106cells.

Applications for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using F0102B in the following applications:

Published Applications

Read 15 publications using F0102B in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG, IGHG1, Immunoglobulin G, ImmunoglobulinG

Product Documents for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody

For research use only

Citations for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody (15)

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Customer Reviews for Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody (3)

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  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 12/17/2020
    Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed twice with PBS and cells were aliquoted into centrifuge tubes for staining. Cells were counted and concentration was adjusted to 10,000 cells per uL. 100 uL of cells was aliquoted into each centrifuge tube for staining.
    Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody F0102B
  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody F0102B
  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 03/24/2017
    106 MA-11 cells were incubated with Mouse anti-Human CD44 antibody (red) or Isotype Control IgG (black), followed by staining with 10 uL of PE-conjugated Goat Anti-Mouse secondary antibody (#F0102B).
    Mouse F(ab)2 IgG (H+L) PE‑conjugated Antibody F0102B

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