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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Flow Cytometry
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F0102B
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Key Product Details

Species Reactivity: Mouse
Immunogen: Mouse IgG heavy and light chains
Applications: Flow Cytometry
Label: Phycoerythrin
Source: Polyclonal Goat IgG
Specificity: Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors

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  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 12/17/2020
    Whole blood was diluted with PBS, overlaid atop Ficoll, and centrifuged to separate the PBMC layer. The PBMC layer was washed twice with PBS and cells were aliquoted into centrifuge tubes for staining. Cells were counted and concentration was adjusted to 10,000 cells per uL. 100 uL of cells was aliquoted into each centrifuge tube for staining.
    Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody Flow Cytometry F0102B
  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody Flow Cytometry F0102B
  • Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 03/24/2017
    106 MA-11 cells were incubated with Mouse anti-Human CD44 antibody (red) or Isotype Control IgG (black), followed by staining with 10 uL of PE-conjugated Goat Anti-Mouse secondary antibody (#F0102B).
    Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody Flow Cytometry F0102B

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