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Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 11711

Product Summary for Mouse IgG1 Isotype Control

Immunogen

KLH

Specificity

Selected for use as mouse IgG1 isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT-II, rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI-309, rhI-Linck, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4,rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1 delta, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha,rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Mouse IgG1 Isotype Control

Detection of Mouse IgG1Isotype Isotype Control by Flow Cytometry

Detection of Mouse IgG1Isotype Control by Flow Cytometry

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or Mouse IgG1Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of Human IgG1 by Flow Cytometry

Detection of Human IgG1 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG1 by Flow Cytometry

Detection of Human IgG1 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Product Documents for Mouse IgG1 Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IgG1 Isotype Control

For research use only

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