Mouse IgG₁ PE-conjugated Antibody

Name: Mouse IgG 1 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC002P
Rating: 5 (10 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # IC002P

Citations (60)

(10)

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Setting the standard in quality research reagents

Key Product Details

Immunogen:	KLH
Applications:	Control
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ kappa Clone # 11711
Specificity:	Selected for use as mouse IgG₁ isotype control for flow cytometry applications.

View Full Technical Details for Mouse IgG₁ PE-conjugated Antibody

Applications for Mouse IgG₁ PE-conjugated Antibody

	Recommended Concentration	Sample
Control	Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse IgG₁ PE-conjugated Antibody

	Control Detection of Mouse IgG Control by Flow Cytometry A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human RANK/TNFRSF11A PE-conjugated Monoclonal Antibody (Catalog # FAB683P, filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody (Catalog # IC002P, open histogram).

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G1
Alternate Names	IgG1, Igh-4, RGD1359539, VH7183

Citations for Mouse IgG₁ PE-conjugated Antibody (60)

Showing 1 - 10 of 60 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IgG₁ PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

VA Stark et al. (2023), Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer bioRxiv : the preprint server for biology, 0(0).
PMID: 36798319
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JT de Kay et al. (2022), Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus Cellular Signalling, 96(0):110360.
PMID: 35609807
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Kleemann et al. (2022), Functional Downregulation of PD-L1 and PD-L2 by CpG and non-CpG Oligonucleotides in Melanoma Cells Cancers, 14(19).
PMID: 36230620
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Sun et al. (2021), Loss of ATF4 leads to functional aging-like attrition of adult hematopoietic stem cells Science Advances, 7(52):eabj6877.
PMID: 34936448
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

D Ali et al. (2021), Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony International Journal of Molecular Sciences, 22(19).
PMID: 34639148
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

S Queckbörne et al. (2020), Endometrial stromal cells exhibit a distinct phenotypic and immunomodulatory profile Stem Cell Res Ther, 11(1):15.
PMID: 31907034
Species: Canine
Sample Types: Whole Cells
Applications: Flow Cytometry

SF Costa et al. (2020), Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis PLoS Negl Trop Dis, 14(1):e0008021.
PMID: 31961868
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

FA Aleisa et al. (2020), Functional Binding of E-selectin to its Ligands is Enhanced by Structural Features Beyond its Lectin Domain J. Biol. Chem., 0(0).
PMID: 31949047
Species: Human
Sample Types: Whole Tissue
Applications: IHC

G Donninelli et al. (2020), Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain J Neuroinflammation, 17(1):149.
PMID: 32375811
Species: Human

Applications: Flow

FM Troschel et al. (2020), Knockdown of Musashi RNA Binding Proteins Decreases Radioresistance but Enhances Cell Motility and Invasion in Triple-Negative Breast Cancer Int J Mol Sci, 21(6).
PMID: 32245259
Species: Human
Sample Types: cell line
Applications: Flow Cytometry

SH Lee et al. (2020), Potentiation of TRAIL?induced cell death by nonsteroidal anti?inflammatory drug in human hepatocellular carcinoma cells through the ER stress?dependent autophagy pathway Oncol. Rep., 44(3):1136-1148.
PMID: 32705218
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Y Dong et al. (2020), Epsin-mediated degradation of IP3R1 fuels atherosclerosis Nat Commun, 11(1):3984.
PMID: 32770009
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

L Shi et al. (2019), Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder Stem Cell Res Ther, 10(1):27.
PMID: 30646947
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

MR Siddiqui et al. (2019), Targeting Epidermal Growth Factor Receptor (EGFR) and Human Epidermal Growth Factor Receptor 2 (HER2) Expressing Bladder Cancer Using Combination Photoimmunotherapy (PIT) Sci Rep, 9(1):2084.
PMID: 30765854
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

F Umrath et al. (2019), Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA Int J Mol Sci, 20(7).
PMID: 30987077
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

MY Hsiao et al. (2019), The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor Int J Mol Sci, 20(14).
PMID: 31336919
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Hyun et al. (2019), HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells PLoS Pathog., 15(7):e1007883.
PMID: 31260499
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

K Walwyn-Bro et al. (2018), Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1 J. Immunol., 0(0).
PMID: 30120122
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

BA Chua et al. (2018), Protein S and Gas6 induce efferocytosis of HIV-1-infected cells Virology, 515(0):176-190.
PMID: 29304470
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytrometry

I De Pauw et al. (2018), Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines Mol Oncol, 0(0).
PMID: 29603584
Applications: Flow Cytometry Control

H Zhang et al. (2017), Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R J. Cell. Mol. Med., 0(0).
PMID: 28922563
Species: Human
Sample Types: Whole Blood
Applications: Isotype Control

(2016), Monitoring PD-L1 positive circulating tumor cells in non-small cell lung cancer patients treated with the PD-1 inhibitor Nivolumab Sci Rep, 6(0):31726.
PMID: 27553175
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Julia Steiger (2016), Imatinib Triggers Phagolysosome Acidification and Antimicrobial Activity against Mycobacterium bovis Bacille Calmette-Guï¿½rin in Glucocorticoid-Treated Human Macrophages J Immunol, 197(1):222-32.
PMID: 27233968
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Pillay S et al. (2016), An essential receptor for adeno-associated virus infection. Nature, 530(7588):108-12.
PMID: 26814968
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control

(2016), IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 90(0):88-95.
PMID: 27863335
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Warat M et al. (2015), The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages. Molecules, 20(1):900-12.
PMID: 25580686
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control

Jarantow S et al. (2015), Impact of Cell-surface Antigen Expression on Target Engagement and Function of an Epidermal Growth Factor Receptor x c-MET Bispecific Antibody. J Biol Chem, 290(41):24689-704.
PMID: 26260789
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ahn S et al. (2015), IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors. J Immunol, 195(6):2591-600.
PMID: 26259585
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Le Q et al. (2015), Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9. J Biol Chem, 290(38):23385-400.
PMID: 26195630
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Fairclough L et al. (2015), Tumour necrosis factor receptor I blockade shows that TNF-dependent and TNF-independent mechanisms synergise in TNF receptor associated periodic syndrome. Eur J Immunol, 45(10):2937-44.
PMID: 26202849
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Anguille S et al. (2015), Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner. PLoS ONE, 10(5):e0123340.
PMID: 25951230
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Huang L et al. (2015), Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells, 33(1):133-45.
PMID: 25187207
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Yamada T et al. (2015), Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib. BMC Cancer, 15(0):260.
PMID: 25885470
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Mercer J et al. (2015), Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis. PLoS ONE, 10(6):e0129776.
PMID: 26075887
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Ekert et al. (2014), Three-dimensional lung tumor microenvironment modulates therapeutic compound responsiveness in vitro--implication for drug development. PLoS ONE, 9(3):e92248.
PMID: 24638075
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control

Mesti T et al. (2014), Metabolic impact of anti-angiogenic agents on U87 glioma cells. PLoS ONE, 9(6):e99198.
PMID: 24922514
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Kim et al. (2014), Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 3(2):172-82.
PMID: 24436439
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry

Wei F et al. (2014), Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing. PLoS ONE, 9(9):e106722.
PMID: 25181476
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Warat M et al. (2014), Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages. Int J Mol Sci, 15(7):11510-22.
PMID: 24979133
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Mancini M et al. (2014), Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells. Br J Cancer, 110(12):2865-73.
PMID: 24853185
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Zhu Z et al. (2014), Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study. PLoS ONE, 9(2):e88775.
PMID: 24558424
Species: Human
Sample Types: Cell Culture Supernates
Applications: Flow Cytometry

Gojo I et al. (2013), Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia. Clin Cancer Res, 19(7):1838-51.
PMID: 23403629
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Mimura et al. (2013), The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer. J Immunol, 191(12):6261-72.
PMID: 24244023
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control

Ray S et al. (2013), The IL-6 trans-signaling-STAT3 pathway mediates ECM and cellular proliferation in fibroblasts from hypertrophic scar. J Invest Dermatol, 133(5):1212-20.
PMID: 23303450
Species: Mouse
Sample Types: Whole Cells
Applications: Control

Andersson S et al. (2012), Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis. PLoS ONE, 7(10):e47668.
PMID: 23082191
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Muller E et al. (2012), Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation. PLoS ONE, 7(5):e38220.
PMID: 22693602
Species: Human
Sample Types: Whole Cells
Applications: Control

Szliszka E et al. (2011), The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells. Int. J. Oncol., 39(4):771-9.
PMID: 21743964
Species: Human
Sample Types: Whole Cells
Applications: Control

Chaturvedi V et al. (2011), Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance. J. Immunol., 186(12):6661-6.
PMID: 21576509
Species: Human
Sample Types: Whole Cells
Applications: Control

Ball SG et al. (2010), Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells. Biochem. J., 427(1):29-40.
PMID: 20102335
Species: Human
Sample Types: Whole Cells
Applications: Control

Heath DJ et al. (2009), Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma. J. Bone Miner. Res., 24(3):425-36.
PMID: 19016584
Inagaki A et al. (2009), Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC. Int. J. Cancer, 125(1):212-21.
PMID: 19358282
Species: Human
Sample Types: Whole Cells
Applications: Control

Sveinbjornsson B et al. (2008), Expression of enzymes and receptors of the leukotriene pathway in human neuroblastoma promotes tumor survival and provides a target for therapy. FASEB J., 22(10):3525-36.
PMID: 18591367
Species: Human
Sample Types: Whole Cells
Applications: Control

Hundhausen C et al. (2007), Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 178(12):8064-72.
PMID: 17548644
Species: Human
Sample Types: Whole Blood
Applications: Control

Vroling L et al. (2007), VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients. Thromb. Haemost., 98(2):440-50.
PMID: 17721629
Species: Human
Sample Types: Whole Cells
Applications: Control

Ball SG et al. (2007), Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 177(3):489-500.
PMID: 17470632
Species: Human
Sample Types: Whole Cells
Applications: Control

Alisa A et al. (2005), Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients. Clin. Cancer Res., 11(18):6686-94.
PMID: 16166448
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Gould WR et al. (2005), Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 3(4):733-41.
PMID: 15733062
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Epelman S et al. (2004), Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs. J. Immunol., 173(3):2031-40.
PMID: 15265938
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Dror Y et al. (2002), Clonal evolution in marrows of patients with Shwachman-Diamond syndrome: a prospective 5-year follow-up study. Exp. Hematol., 30(7):659-69.
PMID: 12135662
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control

Wellemans V et al. (0), Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScienc24(12):103433.
PMID: 34917892

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Customer Reviews for Mouse IgG₁ PE-conjugated Antibody (10)

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Mouse IgG1 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Mouse spleenocytes

Species: Mouse

Verified Customer | Posted 06/08/2022
Mouse IgG1 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: PC-3 human prostate cancer cell line

Species: Human

Verified Customer | Posted 06/07/2022

good control.
Mouse IgG1 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 07/26/2020
Mouse IgG₁ PE-conjugated Antibody

Name: Boubacar Barry

Application: ELISA

Sample Tested: 2120Ep human embryonal carcinoma cell line

Species: Human

Verified Customer | Posted 08/11/2017
Mouse IgG₁ PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Blood-derived monocytes

Species: Human

Verified Customer | Posted 08/01/2017
Mouse IgG₁ PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: U937 human histiocytic lymphoma cell line

Species: Human

Verified Customer | Posted 06/29/2017
Mouse IgG₁ PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Human cell line

Species: Human

Verified Customer | Posted 03/01/2017

FEMX-I Human melanoma cells (10^6) were incubated for 30 minutes at 4ºC with 10 ul of Mouse anti-Human CD9 PE-conjugated antibody (red), or Mouse Isotype Control IgG1-PE (R&D #IC002P, black).
Mouse IgG1 PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: iDC human immature myeloid dendritic cells

Species: Human

Verified Customer | Posted 09/08/2016
Mouse IgG1 PE-conjugated Antibody

Name: Concepcion Estaras

Application: Flow

Sample Tested: H1 human embryonic stem cells treated with GSK3i

Species: Human

Verified Customer | Posted 06/15/2016

H1 cells were treated with ChIR for 48h. 1x106 cells were incubated with 10ul of Mouse IgG1 PE-conjugated (left image) or KDR-PE antibody (right image) for 45min at 4C. Cell were then analyzed using the LSRII analyzer.
Mouse IgG1 PE-conjugated Antibody

Name: Aida Flores

Application: Flow

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 05/17/2016

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Showing 1 - 5 of 10 reviews Showing All

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Mouse IgG₁ PE-conjugated Antibody