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Human/Rat GFAP Antibody
R&D Systems, Inc. a Bio-Techne Brand
Catalog # AF2594
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Key Product Details
| Species Reactivity: | Human, Rat |
| Immunogen: |
E. coli-derived recombinant human GFAP Leu292-Met432 Accession # P14136 |
| Applications: | Immunocytochemistry/Immunofluorescence, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Polyclonal Sheep IgG |
| Specificity: |
Detects human and rat GFAP in Western blots. |
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Western BlotDetection of Human and Rat GFAP by Western Blot. Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Immunocytochemistrybeta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta -III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta -III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern-Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunocytochemistryGFAP in Rat Astrocytes. GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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ImmunocytochemistryGFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Simple WesternDetection of Human GFAP by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Simple WesternDetection of Rat GFAP by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Human/Rat GFAP Antibody (7)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Rat GFAP Antibody include:
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Species: Human
Sample Types: Whole Tissue
Applications: IHCY Su et al. (2022), A single-cell transcriptome atlas of glial diversity in the human hippocampus across the postnatal lifespan Cell Stem Cell, 29(11):1594-1610.e8.
PMID: 36332572 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKM LaBarbera et al. (2021), Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery Journal of neuroscience methods, 0(0):109180.
PMID: 33836174 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCK Wang et al. (2021), Anti-M�llerian Hormone Regulation of Synaptic Transmission in the Hippocampus Requires MAPK Signaling and Kv4.2 Potassium Channel Activity Frontiers in Neuroscience, 15(0):772251.
PMID: 34975379 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PC Liu et al. (2017), APP upregulation contributes to retinal ganglion cell degeneration via JNK3 Cell Death Differ., 0(0).
PMID: 29238071 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKT Gurwitz et al. (2017), Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders Front Mol Neurosci, 10(0):163.
PMID: 28611588 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot(2012), Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 13(0):50.
PMID: 22621398 -
Species: Rat
Sample Types: Whole Cells
Applications: ICCLeonoudakis D et al. (2004), TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity? Neuron Glia Biol., 1(3):263-273.
PMID: 16520832
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