Human GFAP Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136
Leu292-Met432
Accession # P14136
Specificity
Detects human GFAP in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human GFAP Biotinylated Antibody
GFAP in Rat Cortical Stem Cells.
GFAP was detected in immersion fixed differentiated rat cortical stem cells using Sheep Anti-Human GFAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GFAP Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human astrocytes and immersion fixed rat cortical stem cells
Sample: Immersion fixed human astrocytes and immersion fixed rat cortical stem cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human GFAP
Sample: Recombinant Human GFAP
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFAP
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.
Long Name
Glial Fibrillary Acidic Protein
Alternate Names
ALXDRD, FLJ45472, GFAP, GFAP astrocytes, glial fibrillary acidic protein
Gene Symbol
GFAP
UniProt
Additional GFAP Products
Product Documents for Human GFAP Biotinylated Antibody
Product Specific Notices for Human GFAP Biotinylated Antibody
For research use only
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