Sheep IgG Horseradish Peroxidase-conjugated Antibody Best Seller
Catalog # HAF016 | R&D Systems, Inc. a Bio-Techne Brand
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Product Summary for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Immunogen
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Scientific Data Images for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Detection of Sheep IgG Primary Antibody by Western Blot
Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Sheep IgG Primary Antibody by Simple WesternTM.
Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Detection of AIF in Human Kidney
Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Applications for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Immunohistochemistry
Simple Western
Western Blot
Reviewed Applications
Read 14 reviews rated 4.9 using HAF016 in the following applications:
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Stability & Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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Product Documents for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Sheep IgG Horseradish Peroxidase-conjugated Antibody
For research use only
Citations for Sheep IgG Horseradish Peroxidase-conjugated Antibody (42)
Citations are publications that use Bio-Techne products. Selected citations for Sheep IgG Horseradish Peroxidase-conjugated Antibody include:
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Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotX Shu et al. (2023-02-16), Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome Cell Reports, 2023-042(2):112133.
PMID: 36800288 -
Species: Mouse,Human
Sample Types: Tissue Homogenates,Tissue Homogeantes
Applications: Western BlotE Duregotti et al. (2022-11-25), Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity Nature Communications, 2022-113(1):7269.
PMID: 36433953 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotS Gc et al. (2022-11-08), alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth JCI Insight, 2022-17(21).
PMID: 36345944 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotG Untiveros et al. (2022-10-22), Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-123(21).
PMID: 36361539 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotN Pattaracho et al. (2022-09-05), Aquilaria crassna Leaf Extract Ameliorates Glucose-Induced Neurotoxicity In Vitro and Improves Lifespan in Caenorhabditis elegans Nutrients, 2022-014(17).
PMID: 36079924 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotW Chen et al. (2022-07-06), Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion Nature Immunology, 2022-023(7):1021-1030.
PMID: 35794369 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotL Zhou et al. (2022-01-31), Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation Nature Immunology, 2022-023(2):251-261.
PMID: 35102343 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotSM Præstholm et al. (2021-11-02), Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage Cell Reports, 2021-137(5):109938.
PMID: 34731602 -
Species: Human
Sample Types: Protein
Applications: Western BlotT Hoefer et al. (2021-11-01), Targeting shear gradient activated von Willebrand factor by the novel single-chain antibody A1 reduces occlusive thrombus formation in vitro Haematologica, 2021-10(0).
PMID: 33054112 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotPR Elliott et al. (2021-10-05), Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains Cell Reports, 2021-137(1):109777.
PMID: 34610306 -
Species: Cercopithecus aethiops
Sample Types: Cell Lysates
Applications: Western BlotMS Szymczak et al. (2021-08-30), Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays Viruses, 2021-013(9).
PMID: 34578299 -
Species: Mouse
Sample Types: Whole Tissue,Tissue Homogenates
Applications: IHC,Western BlotJ Frew et al. (2021-04-01), Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021-09(1):57.
PMID: 33795008 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCX Zhuang et al. (2020-10-02), CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020-15(19).
PMID: 33004686 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotA Celebi et al. (2020-04-06), Silencing of Wwox Increases Nuclear Import of Dvl proteins in Head and Neck Cancer J Cancer, 2020-011(14):4030-4036.
PMID: 32368285 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Peris-Torr et al. (2020-03-27), Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells Cancers (Basel), 2020-012(4).
PMID: 32230715 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotW Luo et al. (2020-03-06), DNA methylation?regulated miR?155?5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10 Oncol. Rep., 2020-043(5):1692-1704.
PMID: 32323857 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Piro-Mégy et al. (2020-01-02), Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy J. Clin. Invest., 2020-00(0).
PMID: 31550237 -
Species: Mouse
Sample Types: Whole Tissue,Tissue Homogenates
Applications: IHC-Fr,Western BlotK Higashikaw et al. (2019-12-16), Preclinical investigation of potential use of thymidine phosphorylase-targeting tracer for diagnosis of nonalcoholic steatohepatitis Nucl. Med. Biol., 2019-182(0):25-32.
PMID: 31869736 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotSB Drutman et al. (2019-09-04), Homozygous NLRP1 gain-of-function mutation in siblings with a syndromic form of recurrent respiratory papillomatosis Proc. Natl. Acad. Sci. U.S.A., 2019-00(0).
PMID: 31484767 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotA Geisz et al. (2019-06-18), Natural single-nucleotide deletion in chymotrypsinogen C gene increases severity of secretagogue-induced pancreatitis in C57BL/6 mice JCI Insight, 2019-05(0).
PMID: 31211695 -
Species: Gerbil
Sample Types: Tissue Homogenates
Applications: Western BlotTK Lee et al. (2019-06-01), Pretreatment of Populus tomentiglandulosa protects hippocampal CA1 pyramidal neurons from ischemia-reperfusion injury in gerbils via increasing SODs expressions and maintaining BDNF and IGF-I expressions Chin J Nat Med, 2019-017(6):424-434.
PMID: 31262455 -
Species: Human
Sample Types: Whole Tissue
Applications: Western BlotTP Sandberg et al. (2019-03-29), Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer BMC Cancer, 2019-019(1):284.
PMID: 30922247 -
Species: Human
Sample Types: Protein
Applications: Western BlotPJ Stanczyk et al. (2018-08-03), Association of cardiac myosin binding protein-C with the ryanodine receptor channel: putative retrograde regulation? J. Cell. Sci., 2018-00(0).
PMID: 29930088 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotJM Van Kampen et al. (2017-08-24), Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease PLoS ONE, 2017-012(8):e0182896.
PMID: 28837568 -
Species: Human
Sample Types: Protein
Applications: Western BlotY Ge et al. (2017-06-12), UBASH3A Mediates Risk for Type 1 Diabetes through Inhibition of T-Cell Receptor-Induced NF-?B Signaling Diabetes, 2017-00(0).
PMID: 28607106 -
Species: Rat
Sample Types: Protein
Applications: Western BlotS Mary et al. (2017-03-27), Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat Hypertension, 2017-069(5):910-918.
PMID: 28348009 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot(2017-01-03), Targeting tachykinin receptors in neuroblastoma Oncotarget, 2017-00(0).
PMID: 27888795 -
Species: Primate - Chlorocebus sabaeus (Green Monkey)
Sample Types: Cell Lysates
Applications: Western Blot(2016-09-21), Rnd3-induced cell rounding requires interaction with Plexin-B2 J Cell Sci, 2016-00(0).
PMID: 27656111 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr(2016-08-31), Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function J Neurosci, 2016-036(35):9201-16.
PMID: 27581460 -
Species: Human
Sample Types: Protein
Applications: Western BlotNoy P et al. (2016-03-03), Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2). FASEB J, 2016-030(6):2311-23.
PMID: 26939791 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBose C et al. (2015-08-25), Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. PLoS ONE, 2015-010(8):e0136563.
PMID: 26305890 -
Species: Human
Sample Types: Cell Lysates
Nyegaard M et al. (2015-07-21), A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment. PLoS Genet, 2015-011(7):e1005386.
PMID: 26197441 -
Species: Human
Sample Types: Cell Lysates
Applications: ImmunoprecipitationNoy P et al. (2015-03-09), Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth. Oncogene, 2015-034(47):5821-31.
PMID: 25745997 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotAlthoff K et al. (2014-09-01), A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies. Oncogene, 2014-034(26):3357-68.
PMID: 25174395 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotInagaki J et al. (2014-03-11), ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor. Exp Cell Res, 2014-0323(2):263-75.
PMID: 24631293 -
Species: Mouse,Bacteria - E. Coli
Sample Types: Cell Lysates,Whole Tissue
Applications: Western Blot,IHC-PSt Jean A et al. (2014-03-04), Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors. Mol Ther, 2014-022(7):1266-74.
PMID: 24590046 -
Tashima Y et al. (2014-02-26), Antibodies that detect O-linked beta-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem, 2014-0289(16):11132-42.
PMID: 24573683 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotThu K et al. (2013-01-14), SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/beta-catenin signaling. Oncogene, 2013-033(3):279-88.
PMID: 23318427 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotKawato Y et al. (2012-04-12), Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription. PLoS ONE, 2012-07(4):e34703.
PMID: 22511961 -
Species: Ovine
Sample Types: Antibody
Applications: Western BlotNakamura R et al. (2011-12-08), Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions. J. Biol. Chem., 2011-1287(5):3282-91.
PMID: 22157752 -
Species: Mouse
Sample Types: Tissue Homogenates
Bekku Y et al. (2010-01-01), Neurocan contributes to the molecular heterogeneity of the perinodal ECM. Arch. Histol. Cytol., 2010-073(2):95-102.
PMID: 21566336 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Secondary AntibodyPereson S et al. (2009-10-01), Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models. J. Pathol., 2009-1219(2):173-81.
PMID: 19557827
There are no citations that match your criteria.
Customer Reviews for Sheep IgG Horseradish Peroxidase-conjugated Antibody (14)
Customer Images
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Application: ELISASample Tested: Human serumSpecies: HumanVerified Customer | Posted 08/12/2023ELISA for human serum
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Application: Western BlotSample Tested: Cell LysatesSpecies: HumanVerified Customer | Posted 05/27/2022
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Application: Simple WesternSample Tested: Aortic smooth muscle cellsSpecies: HumanVerified Customer | Posted 12/05/2021
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Application: Western BlotSample Tested: MCF-7 human breast cancer cell lineSpecies: HumanVerified Customer | Posted 10/11/2021Dilution: 1:1000 in skim milk in TBST (Incubation: 1hr at room temperature)
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Application: Western BlotSample Tested: C2BBe1 cell lysateSpecies: HumanVerified Customer | Posted 08/16/2021
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Application: Western BlotSample Tested: PC-3 human prostate cancer cell lineSpecies: HumanVerified Customer | Posted 07/30/2021
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Application: Western BlotSample Tested: Recombinant protein and iPS cells-derived neuronSpecies: HumanVerified Customer | Posted 06/23/2020
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Application: Western BlotSample Tested: A549 human lung carcinoma cell lineSpecies: HumanVerified Customer | Posted 05/20/2019
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Application: Western BlotSample Tested: HepG2 human hepatocellular carcinoma cell lineSpecies: HumanVerified Customer | Posted 07/15/2018Western blotting detection of CD47 using sheep polyclonal antibody (AF4670).
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Application: ELISASample Tested: SerumSpecies: SheepVerified Customer | Posted 05/01/2018
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Application: Western BlotSample Tested: H1299 human lung carcinoma cell line and Calu-6 human lung carcinoma cell lineSpecies: HumanVerified Customer | Posted 11/15/2017Primary Ab = Anti SSBP1 Polyclonal Sheep IgG AF6588 (1:200) Secondary Ab = 1:1000
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Application: ELISASample Tested: Human recombinant antibodySpecies: HumanVerified Customer | Posted 10/23/2017
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Application: Western BlotSample Tested: HASMC human aortic smooth muscle cellsSpecies: HumanVerified Customer | Posted 06/13/2017dil: 1:6000 in 5% skim milk powder and TBST.
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Application: Western BlotSample Tested: Tissue sectionsSpecies: SheepVerified Customer | Posted 07/27/2016
There are no reviews that match your criteria.
FAQs for Sheep IgG Horseradish Peroxidase-conjugated Antibody
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Q: Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
A: All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.