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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species▲ Vendor / Cat # Sample Concentration
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
APRIL Goat R&D Systems
AF884
MOLT-4 cells 1:5
BAFF Goat R&D Systems
AF124
MOLT-4 cells 1:10
CCL3/MIP1 Goat R&D Systems
AF-270-NA
Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA
Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405
KPC cells 100 µg/mL
CD4 Goat R&D Systems
AF-379-NA
PBMCs 1:10
CD56 (NCAM-1) Goat R&D Systems
AF2408
Differentiated NK cells 100 µg/mL
CD83 Goat R&D Systems
AF2044
Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA
Dendritic cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410
HEK293T cells 100 µg/mL
DLL4 Goat R&D Systems
AF1389
KPC cells 100 µg/mL
DNMT3A Goat R&D Systems
AF6315
SH-SY5Y 20 µg/mL
E-cadherin Goat R&D Systems
AF748
KPC cells 100 µg/mL
EGFR* Goat R&D Systems
AF231
BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems
AF960
(unpublished) 100 µg/mL
EphB4 Goat R&D Systems
AF446
Neural stem cells 1:20
FOXP3 Goat R&D Systems
BAF3240
Mouse T-regs
GAPDH Goat R&D Systems
AF5718
HT-29 cells 100 µg/mL
GAPDH Goat Novus Biologicals
NB300-320
Jurkat cells
GATA-3* Goat R&D Systems
AF2605
MCF-7 cells 100 µg/mL
GDNF Goat R&D Systems
AF-212-NA
U-87 MG 20 µg/mL
IL-1beta Goat R&D Systems
AF-401-NA
GM-CSF derived BMDCs 10 µg/mL
IL-2Rb Goat R&D Systems
AF-224-NA
PBMCs 400 µg/mL
IRF3* Goat R&D Systems
AF4019
THP1 cells 100 µg/mL
Musashi-1 Goat R&D Systems
AF2628
Transfected Mesenchymal stem cells 100 µg/mL
Oct3-4 Goat R&D Systems
AF1759
Embryonic stem cells 100 µg/mL
Olig2 (O2) Goat R&D Systems
AF2418
25 day glial differentiated neural stem cells 100 µg/mL
PAX2 Goat R&D Systems
AF3364
AN3-CA 20 µg/mL
PAX3 Goat R&D Systems
AF2457
U-87 MG 20 µg/mL
PD1 Goat R&D Systems
AF1086
CD3+ T-cells 100 µg/mL
Smad1 Goat R&D Systems
AF2039
KPC cells 100 µg/mL
Smad2/3 Goat R&D Systems
AF3797
KPC cells 100 µg/mL
SOX1* Goat R&D Systems
AF3369
iPSCs 100 µg/mL
Sox2* Goat R&D Systems
AF2018
NCCIT cells 100 µg/mL
Sox9* Goat R&D Systems
AF3075
Primary murine germ cells 100 µg/mL
TGFbRII Goat R&D Systems
AF-241-NA
HT1080 cells 100 µg/mL
TrkC Goat R&D Systems
AF1404
CHO cells 1:2
VCAM1 Goat R&D Systems
AF643
(unpublished) 100 µg/mL
GAPDH Goat Everest
EB06377
HeLa-GFP cells 100 µg/mL
GAPDH* Goat Sigma Aldrich
SAB2500450
A431 cells 1:10
GFP* Goat Abcam
ab6673
Mouse dendrites 100 µg/mL
Hemoglobin Goat Genetex
GTX77484
Red blood cells 100 µg/mL
Iba1 Goat Abcam
ab48004
Dissociated mouse brain 100 µg/mL
IRF5 Goat Abcam
ab2932
THP1 cells 100 µg/mL
N-Ras* Goat Abcam
ab77392
Patient-derived bladder cancer cells implanted in nude mouse 1:10
OMP Goat Wako Chemicals
019-22291
HepG2 cells 1:10
Sox2 Goat Santa Cruz Biotechnology
sc-17320
Neural stem cells 100 µg/mL
Insulin Guinea Pig EMD Millipore
AB3440
(unpublished) 1:20

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