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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species Vendor / Cat # Sample Concentration▲
FOXP3 Goat R&D Systems
BAF3240
Mouse T-regs
GAPDH Goat Novus Biologicals
NB300-320
Jurkat cells
Histone H3 Rabbit Novus Biologicals
NB500-171
Jurkat cells
Histone H3 dimethyl lys 79 Rabbit Novus Biologicals
NBP2-59194
alpha-actinin Rabbit Cell Signaling Technology
6487
BT474
Cytokeratin 19 Rabbit GeneTex
GTX112666
normal human mammary basal cells
Her2 Mouse Abcam
ab16901
BT474
Her2 Mouse ThermoFisher Scientific
BMS120FI
BT474
Lin28b Rabbit Abcam
ab191881
K562 cells
phospho-S6-ribosomal Rabbit Cell Signaling Technology
5364
BT474
protein species vendor
product_code
sample
S6 ribosomal protein Mouse Cell Signaling Technology
2317
BT474
STAT1 Mouse ThermoFisher Scientific
AHO0832
Treated Jurkat cells
IL-1beta Goat R&D Systems
AF-401-NA
GM-CSF derived BMDCs 10 µg/mL
PABPC4 Rabbit Novus Biologicals
NB100-74593
Human adult bone marrow CD34+ HSPCs  10 µg/mL
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
Atg7 Mouse R&D Systems
MAB6608
HeLa cells 100 µg/mL
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals
NB300-540
Live primary human cortical neurons 100 µg/mL
betaIII-tubulin Mouse R&D Systems
MAB1195
neural differentiated iPSCs 100 µg/mL
CCR7 Rabbit Novus Biologicals
NBP2-58753
Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD209 (DC-SIGN) Sheep R&D Systems
AF161
Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA
Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405
KPC cells 100 µg/mL
CD56 (NCAM-1) Goat R&D Systems
AF2408
Differentiated NK cells 100 µg/mL
CD68 Rat Novus Biologicals
NBP2-33337
RAW264.7 cells 100 µg/mL
CD68/SR-D1 Rat Novus Biologicals
NBP2-80656
RAW264.7 cells 100 µg/mL
CD81 Mouse Novus Biologicals
NBP2-53318
Gli36 cells 100 µg/mL
CD81* Mouse Novus Biologicals
NBP1-44861
Gli36 cells 100 µg/mL
CD83 Goat R&D Systems
AF2044
Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA
Dendritic cells 100 µg/mL
CRBN Rabbit Novus Biologicals
NBP1-91810
HeLa cells 100 µg/mL
Cyclin D1 Rabbit R&D Systems
AF4196
MCF-7 cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410
HEK293T cells 100 µg/mL
Cytochrome b reductase 1 Rabbit Novus Biologicals
NBP2-16075
Red blood cells 100 µg/mL
DLL4 Goat R&D Systems
AF1389
KPC cells 100 µg/mL
E-cadherin Goat R&D Systems
AF748
KPC cells 100 µg/mL
EGFR* Goat R&D Systems
AF231
BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems
AF960
(unpublished) 100 µg/mL
FAK Sheep R&D Systems
AF4467
KPC cells 100 µg/mL
FLAG Tag Rabbit R&D Systems
MAB8529
3T3 Flag-ATF6 cells 100 µg/mL
FOXP3 Rabbit Novus Biologicals
NB100-39002
FoxP3-transfected HEK293T 100 µg/mL
GAPDH Goat R&D Systems
AF5718
HT-29 cells 100 µg/mL
GATA-3 Mouse R&D Systems
MAB6330
MCF-7 cells 100 µg/mL
GATA-3 Mouse R&D Systems
MAB26051
MCF-7 cells 100 µg/mL
GATA-3* Goat R&D Systems
AF2605
MCF-7 cells 100 µg/mL
H3K79me2 Rabbit Novus Biologicals
NBP2-59194
HeLa cells 100 µg/mL
Histone H3 Mouse Novus Biologicals
NBP2-80770
PBMCs 100 µg/mL
Histone H3* Sheep Novus Biologicals
NB100-747
Primary murine germ cells 100 µg/mL

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