Human BAFF/BLyS/TNFSF13B Antibody

Name: Human BAFF/BLyS/TNFSF13B Antibody
Brand: R&D Systems
SKU: AF124
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF124

Citations (11)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B Ala134-Leu285 Accession # Q9Y275
Applications:	Block/Neutralize, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross‑reactivity with recombinant human (rh) APRIL is observed.

View Full Technical Details for Human BAFF/BLyS/TNFSF13B Antibody

Applications for Human BAFF/BLyS/TNFSF13B Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND₅₀) is typically 3-12 ng/mL in the presence of 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B and 1 µg/mL Goat F(ab')2 Anti-mouse IgM.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human BAFF/BLyS/TNFSF13B Antibody

	Neutralization Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND₅₀ is typically 3-12 ng/mL.
	Immunohistochemistry BAFF/BLyS/TNFSF13B in Human Spleen. BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.60 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

Item	Value
References	Schneider, P. et al. (1999) J. Exp. Med. 189:1747. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. Liu, Y. et al. (2002) Cell 108:383. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. Marsters, S.A. et al. (2000) Curr. Biol. 10:785. Thompson, J.S. et al. (2001) Science 293:2108. Ng, L.G. et al. (2004) J. Immunol. 173:807. Roschke, V. et al. (2002) J. Immunol. 169:4314. Batten, M. et al. (2000) J. Exp. Med. 192:1453. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name	B cell Activating Factor
Entrez Gene IDs	10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names	ApoL related ligand TALL-1, B lymphocyte stimulator, B-cell-activating factor, BAFF, BAFFB-cell activating factor, BLYSB-lymphocyte stimulator, BLyS, CD257, CD257 antigen, DTL, Dendritic cell-derived TNF-like molecule, TALL-1delta BAFF, TALL1, TALL1Delta4 BAFF, THANK, TNF and ApoL-related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, ZTNF4, tumor necrosis factor (ligand) superfamily, member 13b, tumor necrosis factor (ligand) superfamily, member 20, tumor necrosis factor ligand superfamily member 13B, tumor necrosis factor superfamily, member 13b, tumor necrosis factor-like protein ZTNF4

Citations for Human BAFF/BLyS/TNFSF13B Antibody (11)

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Citations are publications that use Bio-Techne products. Selected citations for Human BAFF/BLyS/TNFSF13B Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

Y Cao et al. (2020), BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&kappaB signaling pathway in glomerular mesangial cells Mol Med Rep, 21(2):795-805.
PMID: 31974601
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

H Talbot et al. (2020), BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 10(1):12572.
PMID: 32724091
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Imbrechts et al. (2018), IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways Eur. J. Immunol., 0(0).
PMID: 30004580
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

O Berggren et al. (2017), Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype PLoS ONE, 12(8):e0183946.
PMID: 28846748
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Saulep-Easton D et al. (2016), The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 30(1):163-72.
PMID: 26139429
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Hamada T et al. (2015), Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease. BMC Pulm Med, 15(0):110.
PMID: 26424433
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Abe T et al. (2015), The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis. J Immunol, 195(4):1427-35.
PMID: 26150532
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Zhu J et al. (2012), BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. J. Exp. Clin. Cancer Res., 31(0):31.
PMID: 22463935
Species: Human
Sample Types: Whole Cells
Applications: ICC

Kuo SH et al. (2010), Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma. Ann. Hematol., 89(4):431-6.
PMID: 19688210
Species: Porcine
Sample Types: Whole Cells
Applications: ICC

Bergamin F et al. (2007), Essential role of antigen-presenting cell-derived BAFF for antibody responses. Eur. J. Immunol., 37(11):3122-30.
PMID: 17935087
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Elsawa SF et al. (2006), B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia. Blood, 107(7):2882-8.
PMID: 16304043

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Human BAFF/BLyS/TNFSF13B Antibody

Name: Anonymous

Application: IHC

Sample Tested: Spleen tissue

Species: Human

Verified Customer | Posted 02/18/2022

Heat-retrieval in high-pH bufferprimary Ab 10ug/mL for 60minImmPRESS polymer HRP and DAB

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Human BAFF/BLyS/TNFSF13B Antibody

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Applications for Human BAFF/BLyS/TNFSF13B Antibody

Scientific Data Examples for Human BAFF/BLyS/TNFSF13B Antibody

Neutralization

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: BAFF/BLyS/TNFSF13B

Citations for Human BAFF/BLyS/TNFSF13B Antibody (11)

Customer Reviews for Human BAFF/BLyS/TNFSF13B Antibody (1)

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Human BAFF/BLyS/TNFSF13B Antibody

Product Documents for Human BAFF/BLyS/TNFSF13B Antibody

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