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Human BAFF/BLyS/TNFSF13B Antibody

Bio-Techne includes R&D Systems | Catalog # AF124

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AF124
AF124-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human BAFF/BLyS/TNFSF13B Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B
Ala134-Leu285
Accession # Q9Y275

Specificity

Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross‑reactivity with recombinant human (rh) APRIL is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BAFF/BLyS/TNFSF13B Antibody

Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody.

Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody.

In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically 3-12 ng/mL.
BAFF/BLyS/TNFSF13B antibody in Human Spleen by Immunohistochemistry (IHC-P).

BAFF/BLyS/TNFSF13B in Human Spleen.

BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human BAFF/BLyS/TNFSF13B by Immunocytochemistry/ Immunofluorescence

Detection of Human BAFF/BLyS/TNFSF13B by Immunocytochemistry/ Immunofluorescence

Role of p65 activation in BLyS up-regulation. (A) HIF-1 alpha and p65 protein levels in MDA-MB-435 in hypoxic conditions for different time points by Western Blotting. (B) CAPE(50 μM)and PX 12 (10 μM) were used to determine the roles of p65 and HIF-1 alpha in the regulation of BLyS expression by Western Blotting. The cells were treated with or without inhibitor in normoxic or hypoxic conditions for 6 h. (C) Effects of CAPE(50 μM)and PX 12 (10 μM) on BLyS promoter activity. Data were average luciferase activities of three independent transfections with ± SD. *, P < 0.05, vs pGL3-Basic/BP. (D) Localization of p65 protein and expression level of BLyS by immunofluorescence. MDA-MB-435 cells were challenged with CAPE (50 μM) for 6 h (original magnification 200 ×). Image collected and cropped by CiteAb from the following open publication (https://jeccr.biomedcentral.com/articles/10.1186/1756-9966-31-31), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human BAFF/BLyS/TNFSF13B Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Formalin fixed paraffin-embedded sections of human spleen

Western Blot

0.1 µg/mL
Sample: Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)

Neutralization

Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 3-12 ng/mL in the presence of 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B and 1 µg/mL Goat F(ab')2 Anti-mouse IgM.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF124 in the following applications:

Published Applications

Read 14 publications using AF124 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.60 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References

  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
  4. Liu, Y. et al. (2002) Cell 108:383.
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

UniProt

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Human BAFF/BLyS/TNFSF13B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BAFF/BLyS/TNFSF13B Antibody

For research use only

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