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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species Vendor / Cat # Sample Concentration
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
β-tubulin* Rabbit Novus Biologicals
NB600-936
Mouse dendrites 1:40
APRIL Goat R&D Systems
AF884
MOLT-4 cells 1:5
Atg7 Mouse R&D Systems
MAB6608
HeLa cells 100 µg/mL
ATG7 Mouse R&D Systems
MAB6608
Macrophages 1:10
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals
NB300-540
Live primary human cortical neurons 100 µg/mL
BAFF Goat R&D Systems
AF124
MOLT-4 cells 1:10
beta-tubulin Mouse Novus Biologicals
NBP2-50061
Jurkat cells 50 µg/mL
beta-tubulin Mouse Novus Biologicals
NB120-7792
Jurkat cells 50 µg/mL
betaIII-tubulin Mouse R&D Systems
MAB1195
neural differentiated iPSCs 100 µg/mL
Calnexin Rabbit Novus Biologicals
NBP1-97476
U373 MG cells 1:10
CCL3/MIP1 Goat R&D Systems
AF-270-NA
Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CCR7 Rabbit Novus Biologicals
NBP2-58753
Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD11b Rabbit Novus Biologicals
NB110-89474
(unpublished) 1:10
CD209 (DC-SIGN) Sheep R&D Systems
AF161
Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA
Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405
KPC cells 100 µg/mL
CD4 Goat R&D Systems
AF-379-NA
PBMCs 1:10
CD56 (NCAM-1) Goat R&D Systems
AF2408
Differentiated NK cells 100 µg/mL
CD68 Rat Novus Biologicals
NBP2-33337
RAW264.7 cells 100 µg/mL
CD68/SR-D1 Rat Novus Biologicals
NBP2-80656
RAW264.7 cells 100 µg/mL
CD81 Mouse Novus Biologicals
NBP2-53318
Gli36 cells 100 µg/mL
CD81* Mouse Novus Biologicals
NBP1-44861
Gli36 cells 100 µg/mL
CD83 Goat R&D Systems
AF2044
Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA
Dendritic cells 100 µg/mL
CRBN Rabbit Novus Biologicals
NBP1-91810
HeLa cells 100 µg/mL
CRMP5 Rat Novus Biologicals
NB100-74395
1:10
Cyclin D1 Rabbit R&D Systems
AF4196
MCF-7 cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410
HEK293T cells 100 µg/mL
Cytochrome b reductase 1 Rabbit Novus Biologicals
NBP2-16075
Red blood cells 100 µg/mL
DLL4 Goat R&D Systems
AF1389
KPC cells 100 µg/mL
DNMT3A Goat R&D Systems
AF6315
SH-SY5Y 20 µg/mL
E-cadherin Goat R&D Systems
AF748
KPC cells 100 µg/mL
EGFR* Goat R&D Systems
AF231
BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems
AF960
(unpublished) 100 µg/mL
EphB4 Goat R&D Systems
AF446
Neural stem cells 1:20
FAK Sheep R&D Systems
AF4467
KPC cells 100 µg/mL
FLAG Tag Rabbit R&D Systems
MAB8529
3T3 Flag-ATF6 cells 100 µg/mL
FOXP3 Goat R&D Systems
BAF3240
Mouse T-regs
FOXP3 Rabbit Novus Biologicals
NB100-39002
FoxP3-transfected HEK293T 100 µg/mL
GAPDH Mouse Novus Biologicals
NB300-221
freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein 1:40
GAPDH Goat R&D Systems
AF5718
HT-29 cells 100 µg/mL
GAPDH Goat Novus Biologicals
NB300-320
Jurkat cells
GATA-3 Mouse R&D Systems
MAB6330
MCF-7 cells 100 µg/mL
GATA-3 Mouse R&D Systems
MAB26051
MCF-7 cells 100 µg/mL
GATA-3* Goat R&D Systems
AF2605
MCF-7 cells 100 µg/mL
GDNF Goat R&D Systems
AF-212-NA
U-87 MG 20 µg/mL
GFAP Sheep R&D Systems
AF2594
Human iPSCs 1:20
GPCR MRGX2 Rabbit Novus Biologicals
NB110-75035
A549 1:10
H3K79me2 Rabbit Novus Biologicals
NBP2-59194
HeLa cells 100 µg/mL
Histone H3 Rabbit Novus Biologicals
NB500-171
Jurkat cells
Histone H3 Mouse Novus Biologicals
NBP2-80770
PBMCs 100 µg/mL
Histone H3 dimethyl lys 79 Rabbit Novus Biologicals
NBP2-59194
Histone H3* Sheep Novus Biologicals
NB100-747
Primary murine germ cells 100 µg/mL
Histone H3* Mouse Novus Biologicals
NBP2-36468
PBMCs 100 µg/mL
HMGB1 Mouse R&D Systems
MAB1690
HeLa cells 1:5
HSP60 Mouse R&D Systems
MAB1800
iPSCs 100 µg/mL
IL-1beta Goat R&D Systems
AF-401-NA
GM-CSF derived BMDCs 10 µg/mL
IL-2Rb Goat R&D Systems
AF-224-NA
PBMCs 400 µg/mL
iNOS Mouse R&D Systems
MAB9502
U2OS cells 1:10
IRF3 Mouse Novus Biologicals
NBP1-47812
THP1 cells 100 µg/mL
IRF3 Mouse Novus Biologicals
NBP1-04308
THP1 cells 100 µg/mL
IRF3* Goat R&D Systems
AF4019
THP1 cells 100 µg/mL
IRF5* Sheep R&D Systems
AF4508
THP1 cells 100 µg/mL
Lamin B1 Rabbit R&D Systems
AF8525
PBMCs 50 µg/mL
Lin28b Sheep R&D Systems
AF7726
K562 cells 1:10
LY86 Mouse R&D Systems
MAB925
THP-1 and SY-5Y 100 µg/mL
MAP2 Mouse Novus Biologicals
NBP2-61417
Dissociated mouse brain 100 µg/mL
mCherry Mouse Novus Biologicals
NBP2-43769
(mCherry)-HEK293T 100 µg/mL
mCherry Mouse Novus Biologicals
NBP1-96752
(mCherry)-HEK293T 100 µg/mL
MTH1 Rabbit Novus Biologicals
NB100-109
Jurkat cells 100 µg/mL
Musashi-1 Goat R&D Systems
AF2628
Transfected Mesenchymal stem cells 100 µg/mL
MyoD* Mouse Novus Biologicals
NBP2-32883
Satellite Cells 100 µg/mL
Myosin Light Chain 2 (MLC2) Mouse Novus Biologicals
NBP1-28871
1:10
N-Cadherin Sheep R&D Systems
AF6426
KPC cells 100 µg/mL
Neurogenin-2 Mouse Novus Biologicals
H00063973-M10
Transfected Mesenchymal stem cells 100 µg/mL
Oct2 Rabbit Novus Biologicals
NBP1-89417
(unpublished) 100 µg/mL
Oct3-4 Goat R&D Systems
AF1759
Embryonic stem cells 100 µg/mL
OLFM4* Rabbit Novus Biologicals
NBP2-24535
(unpublished) 100 µg/mL
Olig2 (O2) Goat R&D Systems
AF2418
25 day glial differentiated neural stem cells 100 µg/mL
Ovalbumin Rabbit Polyclonal Novus Biologicals
NB600-922
Murine sarcoma cells 1:10
PABPC4 Rabbit Novus Biologicals
NB100-74593
Human adult bone marrow CD34+ HSPCs  10 µg/mL
pAkt Rabbit R&D Systems
AF887
Murine sarcoma cells 100 µg/mL
panCK* Mouse Novus Biologicals
NBP2-29429
BT-20 cells 1:10
PAX2 Goat R&D Systems
AF3364
AN3-CA 20 µg/mL
PAX3 Goat R&D Systems
AF2457
U-87 MG 20 µg/mL
Pax6 Sheep R&D Systems
AF8150
Neural-derived iPSCs 100 µg/mL
PAX8 Rabbit Novus Biologicals
NBP1-32440
786-O cells 50 µg/mL
Pax8* Rabbit Novus Biologicals
NBP1-32440 (lot 39967)
786-O cells 50 µg/mL
Paxillin Sheep R&D Systems
AF4259
KPC cells 100 µg/mL
PD1 Goat R&D Systems
AF1086
CD3+ T-cells 100 µg/mL
pERK1(T202/Y204)/ERK2(T185/Y187) Rabbit R&D Systems
MAB1018
Jurkat +/- 200nM PMA 20 minutes 100 µg/mL
pERK1(T202/Y204)/ERK2(T185/Y187) Rabbit R&D Systems
MAB18251
Jurkat +/- 200nM PMA 20 minutes 100 µg/mL
PHF6 Rabbit Novus Biologicals
NB100-68261
Treated IMR32 cells 100 µg/mL
pHistone H3 Rabbit Novus Biologicals
NB21-1091
(unpublished) 100 µg/mL
pMLKL Mouse R&D Systems
MAB9187
Treated Jurkat cells 100 µg/mL
pSMAD3* Rabbit Novus Biologicals
NBP1-77836
Treated HT1080 cells 100 µg/mL
PTBP-1 Mouse Monoclonal Novus Biologicals
H00005725-M01
U373 cells 1:10
Smad1 Goat R&D Systems
AF2039
KPC cells 100 µg/mL
Smad2/3 Goat R&D Systems
AF3797
KPC cells 100 µg/mL
SOX1* Goat R&D Systems
AF3369
iPSCs 100 µg/mL
Sox2* Goat R&D Systems
AF2018
NCCIT cells 100 µg/mL
Sox9* Goat R&D Systems
AF3075
Primary murine germ cells 100 µg/mL
TCF4 Rabbit Novus Biologicals
NBP1-88633
freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein 1:40
TGFbRII Goat R&D Systems
AF-241-NA
HT1080 cells 100 µg/mL
Thy1/CD90 Sheep R&D Systems
AF2067
SH-SY5Y 20 µg/mL
Thy1/CD90 Sheep R&D Systems
AF7335
SH-SY5Y 20 µg/mL
TrkC Goat R&D Systems
AF1404
CHO cells 1:2
Twist-1 Sheep R&D Systems
AF62301
KPC cells 100 µg/mL
Tyrosine Hydroxylase Sheep Novus Biologicals
NB300-110
Dissociated mouse brain 100 µg/mL
UQCRFS1 Rabbit Novus Biologicals
NBP1-32367
HeLa 100 µg/mL
VCAM1 Goat R&D Systems
AF643
(unpublished) 100 µg/mL
Vinculin Mouse R&D Systems
MAB6896
MCF-7 cells 100 µg/mL
Vinculin Mouse Novus Biologicals
NB600-1293
Treated LNCap cells 1:30
Zeb1 Rabbit Novus Biologicals
NBP1-05987
KPC cells 100 µg/mL
α-actinin4 Mouse Santa Cruz Biotechnology
sc-390205
HeLa cells 100 µg/mL
α-synuclein Rabbit Cell Signaling
4179
SHY5Y cells 1:10
β-tubulin Mouse Genetex
GTX11312
Primary Her2+ breast tumor cells 1:10
β-tubulin* Rabbit Abcam
ab6046
Murine pancreatic cancer cells 1:40
β-tubulin* Mouse Genscript
A-01717-40
Human pluripotent stem cells 1:40
βIII-tubulin (neuronal) Mouse Sigma Aldrich
T8578
Human iPSCs 100 µg/mL
Actin Mouse Developmental Studies Hybridoma Bank
224-236-1
Dictyostelium discoideum 100 µg/mL
Akt (pan) Mouse Cell Signaling
2920
C2C12 cells 1:10
Akt (pan) Rabbit Cell Signaling
4685
C2C12 cells 1:10
Akt (pan)* Rabbit Cell Signaling
4691
C2C12 cells 1:10
Albumin Mouse Abcam
10241
Hepatocyte co-culture 100 µg/mL
Albumin Rabbit Cell Signaling
4929
Hepatocyte co-culture 1:10
ALDH1A1 Rabbit Cell Signaling
12035
(unpublished) 1:10
alpha-actinin Rabbit Cell Signaling Technology
6487
BT474
AML1 Rabbit Cell Signaling
4336
Jurkat cells 1:10
ASC Rabbit Adipogen
AG-25B-0006-C100
GM-CSF BMDCs 50 µg/mL
ATG5 Rabbit Cell Signaling Technology
CST129945
Macrophages 1:10
Atg7* Rabbit Abcam
ab133528
HeLa cells 100 µg/mL
ATP1A3 Mouse Abcam
ab2826
Live primary human cortical neurons 100 µg/mL
Axl Rabbit Cell Signaling
8661
HeLa cells 1:10
Bcl-xL Rabbit Cell Signaling
2764
HeLa cells 1:10
BCL6 Rabbit Cell Signaling
5650
HeLa cells 1:10
BCL6 Rabbit Cell Signaling
14895
HeLa cells 1:10
Blasticidin Rabbit Abcam
ab38307
Transfected HEK293T cells 100 µg/mL
Blimp-1 Rabbit Cell Signaling
9115
U266 cells 1:10
BRM Rabbit Cell Signaling
11966
293T cells 1:10
BTK Rabbit Cell Signaling
8547
Primary sorted human spleen lymphocytes 1:10
BTK Mouse Cell Signaling
56044
Primary sorted human spleen lymphocytes 1:10
c-Jun Rabbit Cell Signaling
9165
KPC cells 1:10
c-Myc Rabbit Cell Signaling
5605
Human pluripotent stem cells 1:10
Calnexin Rabbit Enzo Life Sciences
ADI-SPA-865
U373 MG cells 1:10
Cas9 Mouse Abcam
ab191468
Mouse lymphocytes 1:10
Caspase 8 Mouse Enzo
ADI-AAM-118-E
Treated Jurkat cells 1:10
CD19 Rabbit Cell Signaling
3574
Ramos cells 1:10
CD3 Rat Cell Signaling
4443
MOLT-4 cells 1:10
CD3e Rabbit ThermoFisher Scientific
MA5-14524
Differentiated NK cells 1:10
CD45 Mouse BD Biosciences
555480
PBMCs 100 µg/mL
CD45 Rabbit Cell Signaling
13917
PBMCs 1:10
CD8α Rabbit Cell Signaling
85336
CD3+ T-cells 1:10
CD90 Mouse Abcam
ab23894
Cholangiocarcinoma cells 100 µg/mL
cFos Mouse Santa Cruz Biotechnology
sc-166940
Treated LNCap cells 20 µg/mL
Cleaved caspase 8 Rabbit Cell Signaling
9496
Treated Jurkat cells 1:10
CLIP170 Mouse Santa Cruz Biotechnology
sc-166801
(unpublished) 40 µg/mL
CLIP170 Mouse Santa Cruz Biotechnology
sc-28325
(unpublished) 40 µg/mL
CLIP170* Rabbit Cell Signaling
8977
(unpublished) 1:10
Collagen I Mouse Abcam
ab6308
(unpublished) 100 µg/mL
Cortexillin I Mouse Developmental Studies Hybridoma Bank
241-438-1
Dictyostelium discoideum 1:2
CPEB4 Rabbit Abcam
ab1974928
(unpublished) 100 µg/mL
CPT1A Mouse Abcam
ab128568
(unpublished) 100 µg/mL
CRBN Rabbit Cell Signaling Technology
71810
HeLa cells 1:10
CRMP5 Rat ThermoFisher Scientific
MA3-701
(unpublished) 1:10
CYBRD1 Rabbit Genetex
GTX104793
Red blood cells 100 µg/mL
Cyclin A2 Rabbit Abcam
ab181591
(unpublished) 100 µg/mL
Cytokeratin 14 Mouse ThermoFisher Scientific
MA51599
normal human mammary basal cells 1:10
Cytokeratin 14 Rabbit BioLegend
905301
Mammary tumor organoid cells 1:10
Cytokeratin 14 Rabbit ThermoFisher Scientific
LBVRB-9020-P0
(unpublished) 1:20
Cytokeratin 19 Rat Developmental Studies Hybridoma Bank
TROMA III
(unpublished) 50 µg/mL
Cytokeratin 19 Rabbit GeneTex
GTX112666
normal human mammary basal cells
Cytokeratin 8* Mouse Abcam
ab9023
Breast cancer cells 100 µg/mL
Cytokeratin 8/18* Rat Developmental Studies Hybridoma Bank
TROMA-1-S
Mammary tumor organoid cells 1:2
DDX4 Rabbit Abcam
ab13840
(unpublished) 100 µg/mL
EDNRA Mouse BD Biosciences
611252
(unpublished) 1:20
Efraim teams Sheep Whole sells products
1234567
Products all 1:30
EGFR Mouse Cell Signaling
2232
Circulating tumor cells 1:10
EGFR E746-A750 Rabbit Cell Signaling
2085
HCC827 cells 1:10
EGFR L858R Rabbit Cell Signaling
3197
NCI-H1975 cells 1:10
EGR1 Rabbit Abcam
ab133695
Treated LNCap cells 1:20
eIF4E Rabbit Cell Signaling
2067
Primary Her2+ breast tumor cells 1:10
Eos Rabbit EMD Millipore
ABE1331
HepG2 cells 100 µg/mL
EpCAM Rabbit Cell Signaling
3599
BT-20 cells 1:10
EpCAM Rabbit Abcam
ab213500
(unpublished) 100 µg/mL
ER Rabbit ThermoFisher Scientific
RM-9101-S0
MCF-7 cells 1:10
ERa Rabbit Abcam
ab16660
MCF-7 cells 1:10
ERK 1/2 Mouse Cell Signaling
9107
A172 cells 1:10
ERK1/2 Rabbit EMD Millipore
06-182
Neural stem cells 1:20
ERK1/2* Mouse Cell Signaling
4696
Stimulated PBMCs 1:10
ERK1/2* Rabbit Cell Signaling
4695
Primary Her2+ breast tumor cells 1:10
Etv5 Rabbit Abcam
ab156221
Pedx3-sorted cells 100 µg/mL
FGFR1 Rabbit Cell Signaling
9740
(unpublished) 1:10
FGFR2 Rabbit Cell Signaling
23328
KatoIII cells 1:10
FKBP5 Rabbit Cell Signaling
12210
PBMCs 1:10
FoxL1 Rabbit Abcam
ab95286
A549 cells 1:10
GAPDH Mouse EMD Millipore
MAB374
HeLa cells 1:20

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