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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species Vendor / Cat # Sample▲ Concentration
CRMP5 Rat Novus Biologicals
NB100-74395
1:10
Histone H3 dimethyl lys 79 Rabbit Novus Biologicals
NBP2-59194
Myosin Light Chain 2 (MLC2) Mouse Novus Biologicals
NBP1-28871
1:10
IGF2BP3 Rabbit EMD Millipore
03-198
100 µg/mL
mCherry Mouse Novus Biologicals
NBP2-43769
(mCherry)-HEK293T 100 µg/mL
mCherry Mouse Novus Biologicals
NBP1-96752
(mCherry)-HEK293T 100 µg/mL
mCherry Rat ThermoFisher Scientific
M11217
(mCherry)-HEK293T 100 µg/mL
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
CD11b Rabbit Novus Biologicals
NB110-89474
(unpublished) 1:10
EpCAM* Goat R&D Systems
AF960
(unpublished) 100 µg/mL
Oct2 Rabbit Novus Biologicals
NBP1-89417
(unpublished) 100 µg/mL
OLFM4* Rabbit Novus Biologicals
NBP2-24535
(unpublished) 100 µg/mL
pHistone H3 Rabbit Novus Biologicals
NB21-1091
(unpublished) 100 µg/mL
VCAM1 Goat R&D Systems
AF643
(unpublished) 100 µg/mL
ALDH1A1 Rabbit Cell Signaling
12035
(unpublished) 1:10
CLIP170 Mouse Santa Cruz Biotechnology
sc-166801
(unpublished) 40 µg/mL
CLIP170 Mouse Santa Cruz Biotechnology
sc-28325
(unpublished) 40 µg/mL
CLIP170* Rabbit Cell Signaling
8977
(unpublished) 1:10
Collagen I Mouse Abcam
ab6308
(unpublished) 100 µg/mL
CPEB4 Rabbit Abcam
ab1974928
(unpublished) 100 µg/mL
CPT1A Mouse Abcam
ab128568
(unpublished) 100 µg/mL
CRMP5 Rat ThermoFisher Scientific
MA3-701
(unpublished) 1:10
Cyclin A2 Rabbit Abcam
ab181591
(unpublished) 100 µg/mL
Cytokeratin 14 Rabbit ThermoFisher Scientific
LBVRB-9020-P0
(unpublished) 1:20
Cytokeratin 19 Rat Developmental Studies Hybridoma Bank
TROMA III
(unpublished) 50 µg/mL
DDX4 Rabbit Abcam
ab13840
(unpublished) 100 µg/mL
EDNRA Mouse BD Biosciences
611252
(unpublished) 1:20
EpCAM Rabbit Abcam
ab213500
(unpublished) 100 µg/mL
FGFR1 Rabbit Cell Signaling
9740
(unpublished) 1:10
Glucagon Mouse EMD Millipore
MABN238
(unpublished) 1:10
Glucagon Rabbit Abcam
ab108426
(unpublished) 100 µg/mL
Glucagon Mouse BD Biosciences
565859
(unpublished) 100 µg/mL
GSK3b Mouse Cell Signaling
9832
(unpublished) 1:10
GST Rabbit Cell Signaling
2625
(unpublished) 1:10
Hes1 Rabbit Aviva Systems
ARP32372-T100
(unpublished) 1:5
HMGCS2 Rabbit Sigma Aldrich
SAB2107997
(unpublished) 100 µg/mL
HMGCS2* Rabbit Abcam
ab137043
(unpublished) 100 µg/mL
IGFBP2 Rabbit Abcam
ab91404
(unpublished) 100 µg/mL
IL-18 Rabbit Abcam
ab207324
(unpublished) 1:10
Insulin Guinea Pig EMD Millipore
AB3440
(unpublished) 1:20
Insulin* Guinea Pig Dako
A0564
(unpublished) 1:40
mlc2a Rabbit ThermoFisher Scientific
PA530789
(unpublished) 1:10
mlc2v Mouse Promab
20324
(unpublished) 1:10
NEF Mouse EMD Millipore
MAB899
(unpublished) 1:5
NEF Mouse Abcam
ab42358
(unpublished) 1:5
Oct2* Rabbit Sigma Aldrich
HPA008567
(unpublished) 1:20
Oct4 Mouse Cell Signaling
75463
(unpublished) 1:10
Oct4* Rabbit Cell Signaling
2750
(unpublished) 1:10
OLFM4 Rabbit ThermoFisher Scientific
PA5-73003
(unpublished) 100 µg/mL
OMP* Rabbit LifeSpan Biosciences, Inc.
LS-C462178
(unpublished) 1:10

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