Human/Mouse/Rat alpha -Actinin 1 Antibody

Name: Human/Mouse/Rat alpha -Actinin 1 Antibody
Brand: rnd
SKU: AF8279

Catalog # AF8279

Citations (1)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat
Immunogen:	E. coli-derived recombinant human alpha -Actinin 1 Asp272-Thr500 Accession # P12814
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human, mouse and rat alpha -Actinin 1 in Western blots.

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Applications

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse/Rat alpha -Actinin 1 Antibody Scientific Data Examples

	Western Blot Detection of Human, Mouse, and Rat alpha -Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry alpha -Actinin 1 in A172 Human Cell Line. a-Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: alpha Actinin 1

Alpha-Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa. It is 892 amino acids in length and shares 99% aa identity with mouse and rat ACTN1. Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns. ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Item	Value
Long Name	Actinin alpha 1
Entrez Gene IDs	87 (Human); 109711 (Mouse); 81634 (Rat)
Alternate Names	ACTN1, Alpha-actinin cytoskeletal isoform, BDPLT15, F-actin cross-linking protein, FLJ40884, FLJ54432, Non-muscle alpha-actinin-1, actinin 1 smooth muscle, actinin, alpha 1, alpha Actinin 1, alpha-Actinin 1, alpha-actinin-1, alphaActinin 1

Citations for Human/Mouse/Rat alpha -Actinin 1 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat alpha -Actinin 1 Antibody include:

Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

RC Ahrens-Nic et al. (2019), Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy Sci Adv, 5(9):eaax2066.
PMID: 31517052

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