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Human/Mouse/Rat alpha-Actinin 1 Antibody

Catalog # AF8279 | R&D Systems, Inc. a Bio-Techne Brand
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AF8279
AF8279-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human/Mouse/Rat alpha-Actinin 1 Antibody

Immunogen

E. coli-derived recombinant human alpha-Actinin 1
Asp272-Thr500
Accession # P12814

Specificity

Detects human, mouse and rat alpha-Actinin 1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat alpha-Actinin 1 Antibody

Detection of Human, Mouse, and Rat a-Actinin 1 antibody by Western Blot.

Detection of Human, Mouse, and Rat alpha-Actinin 1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-Actinin 1 antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

alpha-Actinin 1 in A172 Human Cell Line.

a-Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat alpha-Actinin 1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using AF8279 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha Actinin 1

Alpha-Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa.  It is 892 amino acids in length and shares 99% aa identity with mouse and rat ACTN1.  Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns.  ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Long Name

Actinin alpha 1

Alternate Names

ACTN1, alpha-Actinin 1, alphaActinin 1, BDPLT15

Entrez Gene IDs

87 (Human); 109711 (Mouse); 81634 (Rat)

Gene Symbol

ACTN1

UniProt

Product Documents for Human/Mouse/Rat alpha-Actinin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat alpha-Actinin 1 Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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