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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target Host Species Vendor / Cat #▲ Sample Concentration
β-tubulin* Rabbit Abcam
ab6046
Murine pancreatic cancer cells 1:40
Albumin Mouse Abcam
10241
Hepatocyte co-culture 100 µg/mL
Atg7* Rabbit Abcam
ab133528
HeLa cells 100 µg/mL
ATP1A3 Mouse Abcam
ab2826
Live primary human cortical neurons 100 µg/mL
Blasticidin Rabbit Abcam
ab38307
Transfected HEK293T cells 100 µg/mL
Cas9 Mouse Abcam
ab191468
Mouse lymphocytes 1:10
CD90 Mouse Abcam
ab23894
Cholangiocarcinoma cells 100 µg/mL
Collagen I Mouse Abcam
ab6308
(unpublished) 100 µg/mL
CPEB4 Rabbit Abcam
ab1974928
(unpublished) 100 µg/mL
CPT1A Mouse Abcam
ab128568
(unpublished) 100 µg/mL
Cyclin A2 Rabbit Abcam
ab181591
(unpublished) 100 µg/mL
Cytokeratin 8* Mouse Abcam
ab9023
Breast cancer cells 100 µg/mL
DDX4 Rabbit Abcam
ab13840
(unpublished) 100 µg/mL
EGR1 Rabbit Abcam
ab133695
Treated LNCap cells 1:20
EpCAM Rabbit Abcam
ab213500
(unpublished) 100 µg/mL
ERa Rabbit Abcam
ab16660
MCF-7 cells 1:10
Etv5 Rabbit Abcam
ab156221
Pedx3-sorted cells 100 µg/mL
FoxL1 Rabbit Abcam
ab95286
A549 cells 1:10
GFP* Goat Abcam
ab6673
Mouse dendrites 100 µg/mL
Glucagon Rabbit Abcam
ab108426
(unpublished) 100 µg/mL
GRP75 Mouse Abcam
ab82591
U373 MG cells 1:10
H3K27me3 Mouse Abcam
ab205729
Human brain nuclei 100 µg/mL
H3K27me3 Mouse Abcam
ab6002
Treated KARPAS cells 100 µg/mL
Her2 Mouse Abcam
ab16901
BT474
HMGCS2* Rabbit Abcam
ab137043
(unpublished) 100 µg/mL
Iba1 Goat Abcam
ab48004
Dissociated mouse brain 100 µg/mL
IGFBP2 Rabbit Abcam
ab91404
(unpublished) 100 µg/mL
IL-18 Rabbit Abcam
ab207324
(unpublished) 1:10
IRF5 Goat Abcam
ab2932
THP1 cells 100 µg/mL
LANA Rat Abcam
ab4103
Treated B-cells 100 µg/mL
Lin28b Rabbit Abcam
ab191881
K562 cells
LRRK2 Rabbit Abcam
ab133474
Dissociated mouse brain 100 µg/mL
mCherry Rabbit Abcam
ab167453
Mouse dendrites 100 µg/mL
MMP14 Rabbit Abcam
ab51074
Treated MDA-MB-231 100 µg/mL
N-Ras* Goat Abcam
ab77392
Patient-derived bladder cancer cells implanted in nude mouse 1:10
NEF Mouse Abcam
ab42358
(unpublished) 1:5
Nkx2.1 Rabbit Abcam
ab7613
FACS sorted iPSCs 100 µg/mL
Olig2 (O2) Rabbit Abcam
ab136253
Differentiated Oligodendrocyte sample 1:10
Ovalbumin Rabbit Abcam
ab1221
Murine sarcoma cells 1:10
P-glycoprotein Rabbit Abcam
ab170904
PBMCs 1:10
P-glycoprotein* Rabbit Abcam
ab168337
Treated U373 MG cells 100 µg/mL
p53 Mouse Abcam
ab1101
HeLa cells 100 µg/mL
pGSK3β Rabbit Abcam
ab75814
(unpublished) 100 µg/mL
pIRF3* Rabbit Abcam
76493
THP1 cells 100 µg/mL
PKC δ Rabbit Abcam
ab182126
Treated LNCap cells 1:80
PKCα Rabbit Abcam
ab32376
Treated LNCap cells 1:10
pPKCd (Ser299) Rabbit Abcam
ab133456
Treated LNCap cells 1:60
pSMAD3* Rabbit Abcam
ab52903
Treated HT1080 cells 100 µg/mL
PU.1 Rabbit Abcam
ab76543
THP1 cells 100 µg/mL
RFP Mouse Abcam
ab65856
HEK293T cells 100 µg/mL

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