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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (395)
Validated Target▲ Host Species Vendor / Cat # Sample Concentration
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
α-actinin4 Mouse Santa Cruz Biotechnology
sc-390205
HeLa cells 100 µg/mL
α-synuclein Rabbit Cell Signaling
4179
SHY5Y cells 1:10
β-tubulin Mouse Genetex
GTX11312
Primary Her2+ breast tumor cells 1:10
β-tubulin* Rabbit Novus Biologicals
NB600-936
Mouse dendrites 1:40
β-tubulin* Rabbit Abcam
ab6046
Murine pancreatic cancer cells 1:40
β-tubulin* Mouse Genscript
A-01717-40
Human pluripotent stem cells 1:40
βIII-tubulin (neuronal) Mouse Sigma Aldrich
T8578
Human iPSCs 100 µg/mL
Actin Mouse Developmental Studies Hybridoma Bank
224-236-1
Dictyostelium discoideum 100 µg/mL
Akt (pan) Mouse Cell Signaling
2920
C2C12 cells 1:10
Akt (pan) Rabbit Cell Signaling
4685
C2C12 cells 1:10
Akt (pan)* Rabbit Cell Signaling
4691
C2C12 cells 1:10
Albumin Mouse Abcam
10241
Hepatocyte co-culture 100 µg/mL
Albumin Rabbit Cell Signaling
4929
Hepatocyte co-culture 1:10
ALDH1A1 Rabbit Cell Signaling
12035
(unpublished) 1:10
alpha-actinin Rabbit Cell Signaling Technology
6487
BT474
AML1 Rabbit Cell Signaling
4336
Jurkat cells 1:10
APRIL Goat R&D Systems
AF884
MOLT-4 cells 1:5
ASC Rabbit Adipogen
AG-25B-0006-C100
GM-CSF BMDCs 50 µg/mL
ATG5 Rabbit Cell Signaling Technology
CST129945
Macrophages 1:10
Atg7 Mouse R&D Systems
MAB6608
HeLa cells 100 µg/mL
ATG7 Mouse R&D Systems
MAB6608
Macrophages 1:10
Atg7* Rabbit Abcam
ab133528
HeLa cells 100 µg/mL
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
ATP1A3 Mouse Abcam
ab2826
Live primary human cortical neurons 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals
NB300-540
Live primary human cortical neurons 100 µg/mL
Axl Rabbit Cell Signaling
8661
HeLa cells 1:10
BAFF Goat R&D Systems
AF124
MOLT-4 cells 1:10
Bcl-xL Rabbit Cell Signaling
2764
HeLa cells 1:10
BCL6 Rabbit Cell Signaling
5650
HeLa cells 1:10
BCL6 Rabbit Cell Signaling
14895
HeLa cells 1:10
beta-tubulin Mouse Novus Biologicals
NBP2-50061
Jurkat cells 50 µg/mL
beta-tubulin Mouse Novus Biologicals
NB120-7792
Jurkat cells 50 µg/mL
betaIII-tubulin Mouse R&D Systems
MAB1195
neural differentiated iPSCs 100 µg/mL
Blasticidin Rabbit Abcam
ab38307
Transfected HEK293T cells 100 µg/mL
Blimp-1 Rabbit Cell Signaling
9115
U266 cells 1:10
BRM Rabbit Cell Signaling
11966
293T cells 1:10
BTK Rabbit Cell Signaling
8547
Primary sorted human spleen lymphocytes 1:10
BTK Mouse Cell Signaling
56044
Primary sorted human spleen lymphocytes 1:10
c-Jun Rabbit Cell Signaling
9165
KPC cells 1:10
c-Myc Rabbit Cell Signaling
5605
Human pluripotent stem cells 1:10
Calnexin Rabbit Novus Biologicals
NBP1-97476
U373 MG cells 1:10
Calnexin Rabbit Enzo Life Sciences
ADI-SPA-865
U373 MG cells 1:10
Cas9 Mouse Abcam
ab191468
Mouse lymphocytes 1:10
Caspase 8 Mouse Enzo
ADI-AAM-118-E
Treated Jurkat cells 1:10
CCL3/MIP1 Goat R&D Systems
AF-270-NA
Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CCR7 Rabbit Novus Biologicals
NBP2-58753
Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD11b Rabbit Novus Biologicals
NB110-89474
(unpublished) 1:10
CD19 Rabbit Cell Signaling
3574
Ramos cells 1:10

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