Human APRIL/TNFSF13 Antibody

Name: Human APRIL/TNFSF13 Antibody
Brand: rnd
SKU: AF884

Catalog # AF884

Citations (3)

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human APRIL/TNFSF13
Applications:	CyTOF-ready, ELISA, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.

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Applications

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)
ELISA	This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human APRIL/TNFSF13 DuoSet ELISA Kit (Catalog # DY884B) for convenient development of a sandwich ELISA.
Flow Cytometry	2.5 µg/10⁶ cells	Th2-stimulated human PBMC
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human APRIL/TNFSF13 Antibody Scientific Data Examples

	ELISA Human APRIL/TNFSF13 ELISA Standard Curve. Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

Item	Value
References	Planelles, L. et al. (2008) Curr. Mol. Med. 8:829. Hahne, M. et al. (1998) J. Exp. Med. 188:1185. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945. Kelly, K. et al. (2000) Cancer Res. 60:1021. Roth, W. et al. (2001) Cell Death Differ. 8:403. Yu, G. et al. (2000) Nat. Immunol. 1:252. He, B. et al. (2004) J. Immunol. 172:3268. Schwaller, J. et al. (2007) Blood 109:331. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097. Day, E.S. et al. (2005) Biochemistry 44:1919. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964. Hendriks, J. et al. (2005) Cell Death Differ. 12:637. Ingold, K. et al. (2005) J. Exp. Med. 201:1375. Roschke, V. et al. (2002) J. Immunol. 169:4314. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
Long Name	A Proliferation-inducing Ligand
Entrez Gene IDs	8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names	A proliferation-inducing ligand, APRIL, APRILFLJ57090, CD256, CD256 antigen, TALL-2, TALL2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TNFSF13, TRDL-1, TRDL1, ZTNF2, tumor necrosis factor (ligand) superfamily, member 13, tumor necrosis factor ligand superfamily member 13, tumor necrosis factor-like protein ZTNF2, tumor necrosis factor-related death ligand-1

Citations for Human APRIL/TNFSF13 Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human APRIL/TNFSF13 Antibody include:

Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot

H Talbot et al. (2020), BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 10(1):12572.
PMID: 32724091
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 209(10):1813-23.
PMID: 22987802
Species: Human
Sample Types: Serum
Applications: ELISA Development

Moreaux J et al. (2004), BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone. Blood, 103(8):3148-57.
PMID: 15070697

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