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Human/Mouse ATG7 Antibody
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Species Reactivity: | Human, Mouse |
| Immunogen: |
E. coli-derived recombinant human ATG7 Asn387-Gln570 Accession # O95352 |
| Applications: | CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG1 Clone # 683906 |
| Specificity: |
Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed. |
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Western BlotDetection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunocytochemistryATG7 in RAW 264.7 Mouse Cell Line. ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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ImmunohistochemistryATG7 in Human Brain. ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Intracellular Staining by Flow CytometryDetection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
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Simple WesternDetection of Human ATG7 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Citations for Human/Mouse ATG7 Antibody (8)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse ATG7 Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotD Beillevair et al. (2022-01-01), Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes Cell Death Disease, 2022;13(2):145.
PMID: 35149669 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Bernard et al. (2020-01-01), Autophagy drives fibroblast senescence through MTORC2 regulation Autophagy, 0(0):1-13.
PMID: 31931659 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotK Pandey et al. (2020-01-01), Autophagy coupled to translation is required for long-term memory Autophagy, 0(0).
PMID: 32501746 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYH Sheng et al. (2020-01-01), A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32497793 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Jung et al. (2019-01-01), Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits Autophagy, 0(0):1-19.
PMID: 31234698 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryP Thapa et al. (2017-01-01), Histone deacetylase 3 is required for iNKT cell development Sci Rep, 7(1):5784.
PMID: 28724935 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotMaurizio Bossola (2016-01-01), Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia Exp. Gerontol., 0(0).
PMID: 27847330 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBernard M et al. (2014-01-01), Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy, 10(12):2193-207.
PMID: 25495560
There are no citations that match your criteria.
Customer Reviews for Human/Mouse ATG7 Antibody (2)
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Application: IHCSample Tested: Brain tissueSpecies: MouseVerified Customer | Posted 03/23/2022
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Application: IHCSample Tested: Ocular melanomaSpecies: HumanVerified Customer | Posted 09/07/2021
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