Human/Mouse ATG7 Antibody

Name: Human/Mouse ATG7 Antibody
Brand: R&D Systems
SKU: MAB6608
Rating: 5 (2 reviews)

Catalog # MAB6608

Citations (8)

(2)

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Key Product Details

Species Reactivity:	Human, Mouse
Immunogen:	E. coli-derived recombinant human ATG7 Asn387-Gln570 Accession # O95352
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 683906
Specificity:	Detects human ATG7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 10, 12, or 16L1 is observed.

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Applications

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse ATG7 Antibody Scientific Data Examples

	Western Blot Detection of Human ATG7 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry ATG7 in Human Brain. ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunocytochemistry ATG7 in RAW 264.7 Mouse Cell Line. ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Intracellular Staining by Flow Cytometry Detection of ATG7 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
	Simple Western Detection of Human ATG7 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ATG7

ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.

Item	Value
Long Name	ATG7 Autophagy Related 7 Homolog
Entrez Gene IDs	10533 (Human); 74244 (Mouse); 312647 (Rat); 856576 (Yeast)
Alternate Names	APG7, APG7 autophagy 7-like (S. cerevisiae), APG7-LIKE, APG7L, ATG12-activating enzyme E1 ATG7, ATG7, ATG7 autophagy related 7 homolog (S. cerevisiae), Autophagy-related protein 7, DKFZp434N0735, GSA7, Ubiquitin-activating enzyme E1-like protein, hAGP7, ubiquitin activating enzyme E1-like protein, ubiquitin-like modifier-activating enzyme ATG7

Citations for Human/Mouse ATG7 Antibody (8)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse ATG7 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

D Beillevair et al. (2022), Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes Cell Death Disease, 2022;13(2):145.
PMID: 35149669
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Bernard et al. (2020), Autophagy drives fibroblast senescence through MTORC2 regulation Autophagy, 0(0):1-13.
PMID: 31931659
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

K Pandey et al. (2020), Autophagy coupled to translation is required for long-term memory Autophagy, 0(0).
PMID: 32501746
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

YH Sheng et al. (2020), A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy Cell Mol Gastroenterol Hepatol, 0(0).
PMID: 32497793
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S Jung et al. (2019), Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits Autophagy, 0(0):1-19.
PMID: 31234698
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

P Thapa et al. (2017), Histone deacetylase 3 is required for iNKT cell development Sci Rep, 7(1):5784.
PMID: 28724935
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Maurizio Bossola (2016), Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia Exp. Gerontol., 0(0).
PMID: 27847330
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Bernard M et al. (2014), Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy, 10(12):2193-207.
PMID: 25495560

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Customer Reviews (2)

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Human/Mouse ATG7 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Brain tissue

Species: Mouse

Verified Customer | Posted 03/23/2022
Human/Mouse ATG7 Antibody

Name: Anonymous

Application: IHC

Sample Tested: Ocular melanoma

Species: Human

Verified Customer | Posted 09/07/2021

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Human/Mouse ATG7 Antibody

Key Product Details

Complete Your Research

Complete Your Experiment

Applications

Human/Mouse ATG7 Antibody Scientific Data Examples

Western Blot

Immunohistochemistry

Immunocytochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ATG7

Citations for Human/Mouse ATG7 Antibody (8)

Customer Reviews (2)

Customer Images

Human/Mouse ATG7 Antibody

Human/Mouse ATG7 Antibody

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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