Mouse IgG Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # HAF007
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse IgG
Specificity
Detects mouse IgG1, IgG2A, IgG2B, and IgG3 heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with human IgG and rabbit IgG is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IgG Horseradish Peroxidase-conjugated Antibody
Detection of Mouse IgG Primary Antibody by Western Blot
Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Myocilin in human skeletal muscle
Detection of Myocilin in human skeletal muscle using 5 ug/mL of Mouse monoclonal anti-human Myocilin primary antibody (MAB3446) followed by 30 min incubation with Goat anti Mouse HRP conjugated secondary antibodies (Catalog # HAF007) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.Detection of Human IgG by Western Blot
Inverse correlation between the expression of MITF-M and that of miR-155 in melanoma cell lines incubated with IL-1 beta.(A) The expression of MITF-M, Tyrosinase and miR-155 was analyzed by quantitative RT-PCR in 8 melanoma cell lines incubated with IL-1 beta (10 ng/ml) for 4h or 24h. The results were normalized to beta-actin expression for MITF-M and Tyrosinase and to RNU44 for miR-155 (means ± SD for 3 independent experiments). (B) The expression of MITF-M and Tyrosinase proteins was analyzed by Western Blot in the same samples (1 of 3 independent experiments). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25853464), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Use at a 1:20 dilution
Western Blot
Use at a 1:1000 dilution.
Reviewed Applications
Read 16 reviews rated 4.8 using HAF007 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Mouse IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Mouse IgG Horseradish Peroxidase-conjugated Antibody
For research use only
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