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Human/Mouse/Rat STAT3 Antibody
R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: |
E. coli-derived recombinant human STAT3 Met1-Asn175 Accession # P40763 |
| Applications: | Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot |
| Label: | Unconjugated |
| Source: | Monoclonal Mouse IgG2B Clone # 232209 |
| Specificity: |
Detects human, mouse, and rat STAT3 in Western blots. |
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Western BlotDetection of Human STAT3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT3 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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ImmunocytochemistrySTAT3 in HeLa Human Cell Line. STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Intracellular Staining by Flow CytometryDetection of STAT3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. |
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Intracellular Staining by Flow CytometryDetection of STAT3 in HeLa Human Cell Line by Flow Cytometry. HeLa human cell line was stained with Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
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Knockout ValidatedSTAT3 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. STAT3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1, but is not detected in STAT3 knockout (KO) HeLa cell line using Mouse Anti-Human/ Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Knockout ValidatedWestern Blot Shows Human STAT3 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, STAT6 KO HeLa cell line,. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (Catalog # MAB1799) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for STAT3 at approximately 80 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the STAT3 knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Knockout ValidatedSTAT3 Specificity is Shown by Flow Cytometry in Knockout Cell Line. STAT3 knockout HeLa human cervical epithelial cell line was stained with Mouse Anti-Human/Mouse STAT3 Monoclonal Antibody (Catalog # MAB1799, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the STAT3 knockout HeLa cell line was observed. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
Citations for Human/Mouse/Rat STAT3 Antibody (11)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat STAT3 Antibody include:
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Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotJC Kershaw et al. (2022), Piceatannol, a Dietary Polyphenol, Alleviates Adipose Tissue Loss in Pre-Clinical Model of Cancer-Associated Cachexia via Lipolysis Inhibition Nutrients, 14(11).
PMID: 35684106 -
Species: Human
Sample Types: Synovial Fluid
Applications: Western BlotF Caso et al. (2022), Analysis of rheumatoid- vs psoriatic arthritis synovial fluid reveals differential macrophage (CCR2) and T helper subsets (STAT3/4 and FOXP3) activation Autoimmunity reviews, 21(12):103207.
PMID: 36191778 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotKT Tang et al. (2021), Kurarinone Attenuates Collagen-Induced Arthritis in Mice by Inhibiting Th1/Th17 Cell Responses and Oxidative Stress International Journal of Molecular Sciences, 22(8).
PMID: 33924467 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Chakrabort et al. (2021), Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 22(16).
PMID: 34445392 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Chakrabort et al. (2021), ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation International Journal of Molecular Sciences, 22(22).
PMID: 34830441 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYQ Zheng et al. (2019), Opa interacting protein 5 promotes metastasis of nasopharyngeal carcinoma cells by promoting EMT via modulation of JAK2/STAT3 signal Eur Rev Med Pharmacol Sci, 23(2):613-621.
PMID: 30720169 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotX Chen et al. (2019), Circular RNA circHIPK3 modulates autophagy via MIR124-3p-STAT3-PRKAA/AMPK? signaling in STK11 mutant lung cancer Autophagy, 0(0):1-13.
PMID: 31232177 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotH Xu et al. (2018), Tumor-derived mesenchymal-stem-cell-secreted IL-6 enhances resistance to cisplatin via the STAT3 pathway in breast cancer Oncol Lett, 15(6):9142-9150.
PMID: 29844821 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotK Yamada et al. (2017), Inhibition of STAT3 Signaling Reduces IgA1 Autoantigen Production in IgA Nephropathy Kidney Int Rep, 2(6):1194-1207.
PMID: 29270528 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotBruhn M et al. (2014), Proangiogenic tumor proteins as potential predictive or prognostic biomarkers for bevacizumab therapy in metastatic colorectal cancer. Int J Cancer, 135(3):731-41.
PMID: 24374727 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotKolliputi N et al. (2009), IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Am. J. Respir. Cell Mol. Biol., 40(3):314-24.
PMID: 18776134
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Customer Reviews for Human/Mouse/Rat STAT3 Antibody (1)
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Application: ICC/IFSample Tested: Hepatocellular carcinoma cell lineSpecies: HumanVerified Customer | Posted 08/27/2021
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